Method for improving Sudan red I detection kit

A detection kit, Sudan red technology, applied in the field of analytical chemistry, can solve the problems of inapplicability to on-site analysis and long time consumption, and achieve the effects of avoiding false positives and missed detections, saving solvents, and being easy to operate

Inactive Publication Date: 2014-03-19
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Solid phase extraction is widely used due to its good reproducibility, but it takes a long time and is not suitable for on-site analysis

Method used

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  • Method for improving Sudan red I detection kit
  • Method for improving Sudan red I detection kit
  • Method for improving Sudan red I detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The method of the present invention is applied in chili powder.

[0032] Firstly, a composite material of polypyrrole-wrapped magnetic ferric iron tetroxide was synthesized, and the specific synthesis steps were as follows: Synthesize magnetic Fe by solvothermal method 3 o 4 ; 1.0 g magnetic Fe 3 o 4 , 9.1 g FeCl 3 ·6H 2 O and 100 mL of deionized water were added to a 250 mL flask, shaken at 25 °C at 150 r / min for 3 h; then 20 mL of SDS (5.85 wt%) and 0.5 mL of pyrrole monomer were added, and continued to shake for 12 h; then The obtained material was washed successively with water and ethanol until the supernatant was clear, and dried in a vacuum oven at 60 °C for later use.

[0033] Without magnetic solid-phase extraction: Weigh 1.0 g of chili powder into a 10 mL centrifuge tube, add Sudan Red 1 standard (0 μg / g, 6 μg / g, 5 μg / g), 6 mL of n-hexane, shake by hand Let it stand for 1 min, take the supernatant and dry it with an ear suction ball / hair dryer, and detec...

Embodiment 2

[0039] The method of the invention is applied to substrates such as chili oil, spicy sauce, strawberry jam, and soft drinks.

[0040] The sample processing procedure for each matrix is ​​as follows:

[0041] Chili oil: take 1.0 g of chili oil, add 6 mL of n-hexane, shake by hand for 1 min to mix well, let stand, and take the supernatant as the sample solution;

[0042] Spicy sauce: take 1.0 g of spicy sauce, add 6 mL of n-hexane, shake it by hand for 1 min, let stand, and take the supernatant as the sample solution;

[0043] Nongfu orchard mixed fruits and vegetables: Take 1.0 g of beverage, add 6 mL of n-hexane / acetone (4 / 1, v / v), shake by hand for 1 min to mix well, let stand, and take the supernatant as the sample solution;

[0044] Fruity Juice: Take 1.0 g of the beverage, add 6 mL of n-hexane / acetone (4 / 1, v / v), shake it by hand for 1 min, let it stand still, and take the supernatant as the sample solution;

[0045] Tomato paste: weigh 1.0 g tomato paste, add 1 mL H 2 ...

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Abstract

The invention discloses a method for improving a Sudan red I detection kit and belongs to the field of analytic chemistry. According to the method, a sample is pretreated to selectively enrich Sudan red I by a magnetic solid-phase extraction mode and then is detected by the Sudan red I detection kit. The method comprises the following steps of accurately weighing the sample, putting the sample into a container, adding an extraction solvent, oscillating and standing the container, and taking supernate as upper sample liquid; adding the upper sample liquid into a container holding a magnetic solid-phase extraction material, oscillating the container, arranging a magnet on the container, and pouring out the supernate; adding normal hexane, oscillating the container, arranging a magnet on the container, and pouring out the supernate; adding acetone, oscillating the container, dropwise adding the supernate on a sample application plate, and drying the sample application plate in a blowing manner; and executing other operations according to an operating manual of the detection kit. According to the method disclosed by the invention, the Sudan red I in the sample can be effectively and quickly enriched through magnetic solid-phase extraction, so that the detection limit of the kit is reduced by 3 times, and the phenomena of false positive and missing detection are avoided to an extremely large extent. The method has the advantages of simplicity, quickness, easiness in operation and the like, and is suitable for field test, and solvents can be saved.

Description

[0001] technical field [0002] The invention belongs to the field of analytical chemistry, and in particular relates to a method for improving a Sudan red 1 detection kit. Background technique [0003] Sudan dyes are one of the azo dyes, including Sudan Red 1–4, Sudan Red B, Sudan Red 7B, Sudan Red G, Sudan Orange G, Sudan Black, Dimethyl Yellow, and Para Red. Sudan dyes are widely used in scientific research, pigments and cosmetics. At the same time, due to the low price and good coloring effect, Sudan Red 1–4 is used in some foods as an additive to improve the flavor of foods containing chili (such as foods containing chili, curry, palm oil, frozen meat, mixed spices) Shading effect. Studies have shown that Sudan Red 1–4 is carcinogenic to humans and poses a great threat to human health. They are not allowed to be used as food additives. However, there have been incidents of adding Sudan Red to varying degrees in recent years. On February 2, 2005, Premier Foods reporte...

Claims

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Application Information

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IPC IPC(8): G01N21/76G01N1/28G01N1/34
Inventor 冯钰锜许胜男赵琴
Owner WUHAN UNIV
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