7results about How to "Good linear response" patented technology

The invention provides a method, a primer and a probe for qualitatively / quantitatively detecting an astragalus component in a Chinese patent medicine. The method for qualitatively or quantitatively detecting the astragalus membranaceus component in the Chinese patent medicine is constructed on the basis of the specific primer pair, the probe and ddPCR. The quantitative detection method comprises the following steps: extracting DNA (Deoxyribose Nucleic Acid) of a Chinese patent medicine which has a known feed ratio and contains astragalus membranaceus components in different proportions, and carrying out ddPCR amplification reaction based on a specific primer pair and a probe to determine copy number ranges corresponding to the astragalus membranaceus components in different proportions; dNA of the Chinese patent medicine to be detected is extracted, ddPCR amplification reaction is carried out based on the specific primer pair and the probe, and the target DNA copy number of astragalus membranaceus is calculated; and determining the content of the astragalus component in the Chinese patent medicine to be detected through comparison. The method provided by the invention can qualitatively and quantitatively detect the astragalus membranaceus component in the Chinese patent medicine in a high-specificity and high-sensitivity manner, and a new technical means is provided for quality control of Chinese patent medicine bulk drugs.

The invention discloses a fluorescent substrate 7-HC-P-E, a preparation method thereof and application of the fluorescent substrate 7-HC-P-E in detection of the active concentration of alkaline phosphatase (ALP). The fluorescent substrate can be catalytically hydrolyzed by ALP under an alkaline condition to release 7-hydroxycoumarin (7HC) with a strong fluorescent signal, so that high-sensitivity and high-specificity detection on the enzyme activity is realized. The invention further provides a synthesis route and an optimization process of the fluorescent substrate, the operation is simple and convenient, the product purity is high, and the fluorescent substrate is suitable for multiple fields such as biological analysis, medical diagnosis and environmental monitoring.

The invention provides a self-adaptive reconfigurable high dynamic range infrared detector and a control method thereof. The detector comprises a first electrode, an N-type InP layer and an intrinsic InGaAs layer, an annular P-type InP region is arranged on the outer side of the side wall of the intrinsic InGaAs layer, an oxide layer is arranged on the surface of the other side of the intrinsic InGaAs layer, and a transparent electrode is arranged on the oxide layer; and a second electrode is arranged on the P-type InP region. By applying voltage to the transparent electrode, the energy band structure and the carrier transport characteristic of the InGaAs surface can be regulated and controlled: zero bias or positive bias voltage is applied under weak light, and high sensitivity is maintained; negative bias voltage is applied under strong light, a high-concentration hole high-conductivity channel is formed on the surface of the InGaAs in an induction mode, hole collection is accelerated, and charge accumulation and an electric field shielding effect are eliminated. Therefore, linear response in a wide dynamic range is realized under the condition that external attenuation devices are not added.

A porous hollow spherical photonic crystal sensor for detecting xylene gas and a method thereof, comprising synthesizing PS@SiO2 core-shell colloids, assembling into spherical photonic crystals by microfluidics, calcining to remove the polystyrene inner core to form a hollow SiO2 structure, and introducing a porous structure by controllable chemical etching. Subsequently, functionalization is performed by using P(VBC-co-MMA) to obtain a porous hollow spherical photonic crystal sensor, which is endowed with strong molecular affinity for xylene, and selective gas adsorption of the porous photonic crystal is realized. Benefiting from the super-symmetrical spherical structure, the obtained photonic crystal sensor exhibits an inherent angle-independent optical response, overcoming the limitation of angle dependence of thin film photonic crystal sensors. Exposure to xylene gas shows a significant reversible red shift of the optical stop band and a clear color change, which is due to the increase of the effective refractive index caused by vapor diffusion and adsorption. By monitoring the movement of the optical stop band in air and saturated xylene, visual detection of xylene gas can be realized.

The invention discloses a preparation method and application of a Co3O4 / In2O3 heterostructure, and the preparation method at least comprises the following steps: grinding and mixing Co3O4 and In2O3 powder, and calcining to obtain the Co3O4 / In2O3 heterostructure. The preparation method comprises the following steps: firstly, physically mixing Co3O4 and In2O3 by adopting a mechanical grinding method, further calcining the mixed Co3O4 / In2O3 at a high temperature, and enabling Co3O4 and In2O3 to interact under the induction of heat to form a heterostructure. The Co3O4 / In2O3 heterostructure based hydrogen sensor is prepared by taking the Co3O4 / In2O3 heterostructure as a gas sensing film. In hydrogen sensing detection, the response value of the Co3O4 / In2O3 heterostructure-based hydrogen sensor to 10 ppm hydrogen at 200 DEG C can reach 100, and the defect of poor hydrogen sensing performance is overcome.

This invention belongs to the field of biomedical materials and flexible sensing technology, and particularly relates to an implantable pressure sensor, its preparation method, and its application. The pressure sensor has a three-layer parallel plate structure, wherein the electrode layer is a conductive hydrogel prepared from polymerizable zwitterionic monomers, the dielectric layer is a microphase-separated hydrogel formed by copolymerization of acrylamide monomers and acrylate monomers, and the encapsulation layer is a biocompatible polymer fiber membrane. By controlling the material composition, polymerization conditions, and structure, the device achieves good matching with biological soft tissues in terms of water content and tissue adaptability. In terms of functional characteristics, the implantable sensor exhibits advantages such as sensitive pressure response and strong cyclic stability, and can be applied in fields such as tissue engineering, physiological or pathological signal monitoring, neural stimulation modulation, and drug delivery.

The application belongs to the field of fluorescent probes, and discloses a kind of fluorescent probe for monitoring dynamic change of hydrogen peroxide in different subcellular organelles, preparation method and application thereof, the probe is with flavonol as parent nucleus, and hydrogen peroxide ratio type fluorescent probe PML is targeted to lysosome, and hydrogen peroxide ratio type fluorescent probe PZD is targeted to lipid droplet.The application also provides the preparation method and application of the probe.The probe provided by the application has novel structure, simple synthesis method, good recognition effect on hydrogen peroxide, especially hydrogen peroxide in subcellular organelles, fast response speed, high sensitivity, reliable inspection result, good biocompatibility, subcellular organelle positioning function, so that they can be used for monitoring hydrogen peroxide in intracellular microenvironment, and it has great significance to apply probe technology to practical related pathological model to develop the mechanism of hydrogen peroxide in its pathology, especially in the process of ferroptosis.