110results about How to "Synthetic method is fast" patented technology

The invention provides a portable copper ion concentration detection method based on click chemistry and belongs to the field of analytical chemistry. Under the condition that sodium ascorbate reduces cupric into cuprous as a catalyst, a 1,3-dipole cycloaddition reaction is carried out between NDA modified with an azide group and DNA modified with an alkynyl group, so that DNA modified with cane sugar invertase is fixed on magnetic beads; The magnetic beads are separated from a solution, the cleaned magnetic beads are dissolved into a cane sugar solution to carry out enzymatic hydrolysis, and then a reaction solution after enzymatic hydrolysis is measured by adopting a blood glucose meter, so that copper ion concentration can be detected. The portable copper ion concentration detection method provided by the invention takes the blood glucose meter as a platform and can portably and quickly detect the copper ion concentration on site; and the portable copper ion concentration detection method can be applied to copper ion detection in a human body serum sample, and the portable copper ion concentration detection method has the advantages of easy operation, low cost, high sensitivity and good specificity.

The invention relates to a protein self-assembled novel nanovaccine and a preparation method thereof. The protein self-assembled novel nanovaccine is prepared on the basis of antigen protein self-assembly; in the process of vaccine preparation, a molecular adjuvant is selectively introduced, the antigen content is higher than or equal to 85%, a high-efficiency immune effect can be triggered without needing assistance of an aluminum adjuvant, a Freund's adjuvant and the like, mercapto groups between protein molecules are exposed by virtue of physical regulation and control, and stable protein nanoparticles mainly based on disulfide bond crosslinking are formed through a mercapto/disulfide bond exchange reaction. The defects that the conventional nanovaccine needs to be introduced with an exogenous carrier or a cross-linking agent and the like are overcome, and the immune effect and the biosafety of the vaccine can be improved at the same time; the obtained vaccine granules are tidy in morphology, high in stability, flexible in regulation and control mode and good in repeatability, and can effectively stimulate dendritic cell maturation; the protein self-assembled novel nanovaccine has relatively strong generality and universality, is verified in a series of antigen proteins, and has a potential significant application value in the fields of novel vaccinating methods and biological pharmacy.

The invention discloses a portable method for detecting content of adenosine triphosphate (ATP). The method comprises the following steps of: respectively modifying a magnetic bead and invertase on an ATP aptamer which is split into two sections, performing specific identification and combination on the ATP aptamer which is split into two sections and a target ATP so as to form a sandwich structure, so that the aptamer modified with the invertase is fixed on the magnetic bead; separating the magnetic bead from the solution, dissolving the cleaned magnetic bead in a sucrose solution for carrying out an enzymatic hydrolysis reaction, measuring the reaction solution subjected to enzymatic hydrolysis by employing a glucometer, and indirectly detecting the concentration of ATP. The method is successfully applied to detection of the ATP concentration in a human serum sample, and the method has the advantages of simple operation, low cost, high sensitivity, high specificity and the like.

The invention discloses a method for synthesizing full-silicon beta zeolite with small crystal grains. The method comprises the following steps of: (a) mixing a silicon source, a fluorine ion source, a tetraethyl ammonium ion source and deionized water according to a specific formation mole ratio to form a reaction mixture; (b) carrying out a crystallization reaction on the reaction mixture untilthe full-silicon beta zeolite the grain size of which is smaller than 5mum is formed; and (c) recovering the full-silicon beta zeolite. By utilizing the method, full-silicon beta zeolite crystals with small crystal grains can be synthesized in a short time, and the yield of the full-silicon beta zeolite crystals with small crystal grains is greater than 90 percent. The full-silicon beta zeolite with small crystal grains, which is synthesized by the method, can be used for manufacturing a zeolite film or can be used as a carrier used for loading a catalyst.

The invention discloses a preparation method and application of a polyacrylic acid hydrogel adsorbing material. The polyacrylic acid hydrogel adsorbing material is characterized in that crylic acid serves as a monomer, N,N'-methylene bisacrylamide serves as a cross-linking agent, ammonium persulfate serves as an initiator, sodium hydrogen sulfite serves as a coagulant aid, and the polyacrylic acid hydrogel adsorbing material is formed by free radical polymerization. By means of the preparation method and application, the synthetic method is simple and convenient, the requirement for the reaction condition is low, the price of raw materials is low, and utilization and popularization are facilitated; synthesis and adsorbing operation steps are simple and convenient; the polyacrylic acid hydrogel adsorbing material can effectively absorb heavy metal ions such as uranium and copper so as to achieve the aim of reasonably treating waste water; and the optimal adsorption conditions can be obtained by adjusting the different initial concentration, the optimal PH and the absorbing balance time.

The invention discloses a method for preparing a zinc oxide by using an ultrasonic synthesis method. The method comprises the following steps: respectively preparing a strong alkali solution and a zinc salt solution with the same volumes; respectively carrying out preheating treatment on the two solutions at the temperature of 0-100 DEG C; putting the strong alkali solution into an agitator blade, carrying out mechanical stirring, and simultaneously carrying out ultrasonic dispersion; injecting the zinc salt solution into the strong alkali solution, and further carrying out ultrasonic stirring for 10-60 seconds to obtain a mixture; standing and participating the mixture for 2-3 hours, filtering out an upper clear liquid, adding deionized water and further washing until the pH value of the upper clear liquid is neutral; washing with absolute ethyl alcohol; and finally drying to obtain solid powder. The synthesis method disclosed by the invention is simple and fast; zinc salt can be prepared by participation reaction in a water solution under the alkaline condition; and the prepared zinc oxide is applied to a dye-sensitized solar cell light anode, so that diffusion and permeation of quasi-solid electrolyte are facilitated; the relatively large specific surface area can be provided; and the adsorbing capacity of dyes is increased.

The invention discloses a preparation method of magnetic composite hydrogel for copper ion adsorption. The preparation method comprises: deacetylating cellulose acetate nanofibers to obtain cellulosenanofibers, dissolving acrylic acid, maleic anhydride and N, N'-methylene bisacrylamide in a nitric acid solution to obtain a reaction solution A, dissolving ammonium cerium nitrate in the nitric acidsolution, adding the cellulose nanofibers and vinyl-modified silica into the solution to obtain a reaction solution B, adding the reaction solution A into the reaction solution B drop by drop for a reaction so that cellulose nanofiber-grafted poly(acrylic acid-co-maleic anhydride)hydrogel is obtained, dissolving ferrous chloride and ferric chloride in deionized water, adding the cellulose nanofiber-grafted poly(acrylic acid-co-maleic anhydride)hydrogel and ammonia water into the solution and carrying out a reaction process. The preparation method is simple and fast, has strong operability andis very suitable for industrial production.

The invention discloses a reduction/pH double response adriamycin prodrug and a preparation method and application thereof. The specific preparation method comprises the steps of performing a chemical reaction by using a small molecule compound 3,3'-dithio diyne butyl dipropionate (B-ss-B) endcapped with alkynyl and a compound diazide ethyl diacetal polyethyleneglycol (N3-a-PEG-a-N3) endcapped with an azide group, so as to prepare a reduction/pH double response polymer poly(SS-alt-A)n endcapped with alkynyl; and then connecting adriamycin derivatives at two ends of a polymer chain, so as to obtain the reduction/pH double response adriamycin prodrug DOX-hyd-poly(SS-alt-A)n-hyd-DOX. The reduction/pH double response water-soluble adriamycin prodrug has good biocompatibility and characteristics of controlled-release drugs, so that the reduction/pH double response water-soluble adriamycin prodrug can be used as a stimulating sensitive antitumor prodrug.

The invention discloses a catalyst containing palladium compound, a preparation method and an application thereof. According to a compounding method of the palladium compound PdBr2(PPh3)2, o-chloro pyridine is taken as an initial raw material, reacts with methyl iodide and then reacts with tetra (triphenylphosphine) palladium. According to the compounding method of the palladium compound PdI2(PPh3)2, o-bromopyridine is taken as the initial raw material, reacts with iodoacetic acid and then reacts with tetra (triphenylphosphine) palladium. The method for compounding the target product provided by the invention is simple, easy to operate and control and also has the advantages of high product purity and high yield. Such a compound can be applied to the Heck coupling reaction of aryl halogen and ethyl acrylate and has ultrahigh catalytic activity.

The invention discloses a hydrophilic group modified two-dimensional magnetic nano material and a preparation method thereof, and application of a hydrophilic group modified two-dimensional magnetic nano material. The material is prepared from a two-dimensional molybdenum disulfide nano material, magnetic nanoparticles connected to the surface of the two-dimensional molybdenum disulfide nano material through metal covalent bonds, nanogold wires polymerized on the surface of the two-dimensional molybdenum disulfide nano material in an Au-S bond mode, and a hydrophilic group reagent polymerizedon the surface of the nanogold wires in an Au-S bond mode. Through hydrophilic interaction and electrostatic interaction between a group reagent in the material and a glycosylated peptide fragment, efficient selective enrichment of glycopeptides is achieved; and moreover, the glycosylated peptide fragment is good in enrichment effect, and enrichment detection of glycopeptides in low-concentrationhuman serum albumin (such as IgG) and human urine exosome protein can be achieved.

There is provided a method of synthesizing heat elimination mastic, its producing procedure comprises the following steps: providing the filled powder and the base materials of the mastic; charging the mentioned filled powder and base materials into a container while keeping the base material in the state of melting or liquidness; pressurizing and mixing the mixture to mix them uniformly. The method has the advantage of making the heat elimination mastic mix quickly and effectively.

The invention discloses an isoquinoline compound and a synthetic method thereof. The isoquinoline compound is shown in the formula I (please see the formula in the description), wherein R1 represents a hydrogen atom, a halogen atom, an alkyl group, an alkoxy group or an amino group, and R2 represents a hydrogen atom, a halogen atom, an alkyl group, an alkoxy group or an amino group. The target product is synthesized through a three-step reaction, the synthesis method is simple and rapid, the target compound is high in yield, and the necessary foundation is provided for further research on the compound of the formula I or relevant compounds.

Relating to a preparation method and application of a novel nanocomposite material, the invention provides a preparation method of a mesoporous silica-restricted gold nanocluster-induced fluorescence enhanced nano-fluorescent device and application of the same in copper ion detection, and aims to solve the problems of high cost, complex process and long detection time in existing copper ion detection methods. The method includes: 1. taking chloroauric acid as the raw material, adopting reduced glutathione as the reducing agent and stabilizer, and utilizing thermal reduction means to synthesize a gold nanocluster solution A from the bottom up under a high temperature stirring condition, loading the solution A into a centrifugal tube for centrifugation, taking the precipitate, using a mixed solution of acetonitrile and water to perform washing 3 times and performing dissolving in deionized water to obtain a solution B; 2. making TEOS, ethanol, water and ammonia water into solid silica particles by Stober process, washing and drying the silica particles, then taking NaoH as the etchant, and performing sealed stirring overnight in a flask, then loading the obtained solution into a centrifugal tube for centrifugation 3 times to remove the precipitate left by the supernatant, and performing washing and drying to obtain mesoporous silica; and 3. putting the mesoporous silica into the solution B under a stirring condition so as to obtain the novel nanocomposite material with specific detection application in cooper ions. The composite material prepared by the method has the characteristics of low cost, simple operation, rapid detection and high sensitivity, and can be used in the fields of environmental ion detection and bioimaging.

The invention relates to the technical field of functionalized magnetic nano materials, and provides a composite magnetic nano material based on DNA tetrahedrons, preparation and application. The preparation method comprises the following steps of synthesizing DNA tetrahedrons through a self-assembly reaction of a DNA single chain, loading magnetic nano particles on the surface of molybdenum disulfide particles, modifying gold nanoparticles on exposed active sulfur atoms of molybdenum disulfide particles, modifying the DNA tetrahedrons on the gold nanoparticles, and incubating and connecting protein antibodies on the DNA tetrahedrons. The material prepared by adopting the preparation method is used for enriching and detecting the low-abundance protein in the serum, the specific low-abundance protein in the serum can be efficiently and highly selectively enriched through a specific reaction between an antigen and an antibody, and the material takes a magnetic nano material as a matrix, so that the material has the advantages of simplicity, convenience and rapidness in use, the treatment time of the serum complex matrix is greatly shortened, and high-efficiency and high-selectivity enrichment of low-abundance proteins in the complex matrix can be realized.

The invention relates to a method for preparing a nanometer nickel pellet with magnetic property. Nickel dichloride, polyvinylpyrrolidone, potassium hydroxide and sodium hypophosphite are dissolved in water respectively; a nickel dichloride solution and a potassium hydroxide solution are mixed, are introduced with nitrogen gas, then added with a sodium hypophosphite solution and continuously kept in a nitrogen introducing state for 10 minutes; the mixed solution is transferred to an inner tank of a hydrothermal kettle with cubage of 15 ml and is continuously introduced with nitrogen gas for protection; the hydrothermal kettle is sealed; the mixed solution reacts for 40 to 60 minutes at a temperature of between 130 and 150 DEG C; and an obtained product is washed by water and ethanol respectively, subjected to magnetic separation and is dried to obtain the nanometer nickel pellet with the magnetic property. The nanometer nickel pellet is subjected to characteristic representation through x ray diffraction (XRD), a transmission electron microscope (TEM) and a scanning electron microscope (SEM); the result shows that the particle diameters of a series of prepared nanometer nickel pellets are between 100 and 500 nanometers; and the particle diameter is relevant to reaction temperature, PH value, dosage of a reducer, reaction time and an added surfactant.

The invention discloses a preparation method and application of hydrophobic graphene aerogel. The invention belongs to the field of graphene aerogel. The problems that an existing hydrophobic grapheneaerogel material is high in preparation cost, poor in structural strength and the like can be solved. A hydrothermal reaction method is adopted, a saccharide cross-linking agent which is low in priceand easy to obtain is adopted, an organic auxiliary agent and efficient and cheap modifiers such as trimethylchlorosilane are added, and the hydrophobic and oleophylic graphene aerogel material is obtained through a normal-pressure drying method and a microwave annealing method. It can be seen through scanning electron microscope imaging that the material is structurally a thin-wall porous material formed by three-dimensional crosslinking of thin-layer graphene. The material is definite in synthesis process, short in synthesis time, low in cost, free of heavy metal and organic pollutants, high in structural strength and capable of bearing the heavy pressure which is more than one hundred times of the mass of the material. The material has super-hydrophobicity, and the contact angle measured by experiments is as high as 150 degrees or above. The hydrophobic graphene aerogel can be used in the fields of protection and removal of volatile organic compounds in air and water, and other oil-water separation applications.

The invention relates to a manganese dioxide/carbon paper composite electrode material and a preparation method thereof, belonging to the technical field of a super-capacitor electrode material. The material adopts carbon paper as a matrix (a current collector), a manganese dioxide nano sheet is attached to the surface of the carbon paper, the length of the manganese dioxide nano sheet is 150 to 200 nm, and the thickness of the manganese dioxide nano sheet is 5 to 15 nm. The preparation method comprises the following steps of: firstly sufficiently and uniformly mixing potassium permanganate, ammonium fluoride and deionized water according to a given ratio to obtain a mixed system, immersing the carbon paper in the mixed system, performing the reaction, and finally washing and drying a reaction product. The preparation method is simple, rapid and capable of realizing the mass production; the obtained composition material is controllable in size, regular in shape, good in electrochemical performance, particularly high in capacitance and good in cycling performance, the solves the technical problems of the prior art that the structure is unstable and the multiplying power performance is poor, and is particularly suitable for a super-capacitor material; in addition, the composite material can also be applied to a flexible device.

The invention discloses an efficient synthesis method of a rhodium nitrate solution. The basic principle is a three-step synthesis method; in the first step, rhodium powder is dissolved to obtain a chlororhodic acid solution; in the second step, chlororhodic acid is converted into rhodium hydroxide precipitates; in the third step, the rhodium hydroxide precipitates are dissolved in nitric acid toobtain the rhodium nitrate solution. Compared with the prior art, the efficient synthesis method has the advantages that through a specific pre-grinding technology, the full mixing of the rhodium powder and barium peroxide is ensured, so that the goal that the rhodium powder is almost completely converted into soluble oxides by using the barium peroxide being 3 to 3.5 times of the weight of the rhodium powder is realized; the conversion rate reaches 99.7 percent or higher; meanwhile, the efficient solid and liquid separation technology is combined; the impurity content in the rhodium nitrate solution is enabled to be low enough.

The invention discloses a method for quantitatively detecting alanine transaminase in a solution based on a copper nanocluster fluorescent probe, wherein a copper nanocluster with glutathione as a protective agent is used as a fluorescent probe, and the content of alanine transaminase in the solution is specifically detected through a fluorescence "off-on" mode. The label-free, highly sensitive and selective detection of alanine transaminase is achieved by adopting the fluorescence "off-on" mode. The method is simple and rapid, the specific detection of alanine transaminase can be realized, the detection linear range is wide, and the detection limit is low. The method in the invention has good detection sensitivity and selectivity and has good application prospects in disease detection, clinical application and other aspects.

The invention relates to a method for synthesizing a phosphorus-oxychloride-promoted amide compound. The synthesizing method includes the steps that carboxylic acid serves as one reactant, another reactant (N,N-dialkyl methanamide) serves as a solvent, one equivalent of phosphorus oxychloride is added, and the amide compound is prepared. The reaction substrates are low in price and easy to get, the nature is stable, toxicity is small, the reaction speed is high, conditions are moderate, and the reaction substrates can be widely applied to substrates with different functional groups. The efficiently-constructed amide compound is an important molecular skeleton for many medicines, bioactive molecules and natural products, and the synthesizing method is a widely-applicable preparing method for synthesizing the compound.

The invention discloses a near-infrared fluorescent magnetic Fe NCs dual-mode probe as well as a synthesis method and an application thereof. The valence state of an iron element in the magnetic Fe NCs dual-mode probe is zero, the particle size of the iron element is 5 nm, an iron nano-cluster prepared from glutathione serving as a stabilizer and iron ions in a molar ratio of 1: 0.3073 has excellent near-infrared fluorescence emission capacity, interference of biological autofluorescence in a sample can be effectively avoided, and the product can be used for high-sensitivity detection of the drug molecule tiopronin in a complex detection system. Besides, the probe also has relatively strong paramagnetic properties, can generate bright T1 weighted image signals under the action of an external magnetic field, is gradually enhanced along with the increase of the concentration of the probe, and also has a wide application prospect in the aspect of magnetic resonance imaging.