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Composite magnetic nano material based on DNA tetrahedrons, preparation and application

A technology of nanomaterials and composite magnetism, which is applied in the direction of magnetic materials, diamagnetic/paramagnetic materials, magnetic objects, etc., can solve the problems of interfering with the detection of low-abundance proteins, and achieve clean material synthesis methods and highly selective enrichment Effect

Active Publication Date: 2021-06-08
NAT INST OF METROLOGY CHINA
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Problems solved by technology

[0003] Malignant tumor markers in serum are usually low-abundance proteins (for example, the content of liver cancer marker HSP90α in blood is usually only about 60 ng / mL), but the protein types in serum are complex and diverse, and the existence of high-abundance proteins will seriously interfere Detection of low-abundance proteins

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  • Composite magnetic nano material based on DNA tetrahedrons, preparation and application
  • Composite magnetic nano material based on DNA tetrahedrons, preparation and application
  • Composite magnetic nano material based on DNA tetrahedrons, preparation and application

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preparation example Construction

[0040] Such as figure 1 Shown, the embodiment of the present invention a kind of preparation method based on the composite magnetic nano material of DNA tetrahedron, comprises the following steps:

[0041] S1. Load ferroferric oxide magnetic nanoparticles on the surface of molybdenum disulfide particles to obtain the product I:MoS 2 @Fe 3 o 4 ;

[0042] S2. Using the interaction of the "Au-S" bond, modify the gold nanoparticles on the exposed active sulfur atoms on the product I to obtain the product II: MoS 2 @Fe 3 o 4 @AuNPs;

[0043] S3. Utilize the interaction of "Au-S" bond to modify the DNA tetrahedron with three vertices containing sulfhydryl groups on the gold nanoparticles in the product II to obtain the product III: MoS 2 @Fe 3 o 4 @AuNPs @DNA TET;

[0044] S4, activating the carboxyl group modified on the DNA tetrahedron in the product III, incubating the activated product III with the protein antibody solution to make the protein antibody connect to the c...

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Abstract

The invention relates to the technical field of functionalized magnetic nano materials, and provides a composite magnetic nano material based on DNA tetrahedrons, preparation and application. The preparation method comprises the following steps of synthesizing DNA tetrahedrons through a self-assembly reaction of a DNA single chain, loading magnetic nano particles on the surface of molybdenum disulfide particles, modifying gold nanoparticles on exposed active sulfur atoms of molybdenum disulfide particles, modifying the DNA tetrahedrons on the gold nanoparticles, and incubating and connecting protein antibodies on the DNA tetrahedrons. The material prepared by adopting the preparation method is used for enriching and detecting the low-abundance protein in the serum, the specific low-abundance protein in the serum can be efficiently and highly selectively enriched through a specific reaction between an antigen and an antibody, and the material takes a magnetic nano material as a matrix, so that the material has the advantages of simplicity, convenience and rapidness in use, the treatment time of the serum complex matrix is greatly shortened, and high-efficiency and high-selectivity enrichment of low-abundance proteins in the complex matrix can be realized.

Description

technical field [0001] The invention relates to the technical field of functionalized magnetic nanometer materials, in particular to a composite magnetic nanometer material based on DNA tetrahedron, its preparation and application. Background technique [0002] Malignant tumors have become a disease with high morbidity and high mortality that seriously affects human health. In my country, the incidence of malignant tumors maintains an annual increase of about 3.9%, and the mortality rate maintains an annual increase of 2.5%. Relevant data show that 1 / 3 of cancers can be cured through early detection. However, many cancer patients in our country are already in the middle and late stages once they are discovered, making treatment difficult. Therefore, the development of tumor markers for early cancer diagnosis is of great significance for the diagnosis and treatment of cancer. [0003] Malignant tumor markers in serum are usually low-abundance proteins (for example, the conte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68H01F1/00H01F41/02
CPCG01N33/6848H01F41/02H01F1/0018G01N33/5434G01N33/54346G01N33/68G01N33/54326
Inventor 楚占营翟睿朱曼曼赵洋龚晓云谢洁武利庆江游戴新华方向
Owner NAT INST OF METROLOGY CHINA
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