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A kind of surface polymerized ionic liquid modified magnetic nanomaterial and its preparation method and application

A technology of polymerizing ionic liquids and magnetic nanometers, which is applied in the preparation of test samples, ion exchange, ion exchange regeneration, etc., can solve the problems of limited selectivity and low sensitivity, and achieve simple synthesis methods and good glycopeptide enrichment effects , the effect of high-efficiency selective enrichment

Active Publication Date: 2019-09-27
ACADEMY OF MILITARY MEDICAL SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the hydrophilic material used in the enrichment of glycopeptides by hydrophilic interaction chromatography is usually a nanomaterial with a surface-modified hydrophilic group layer, which has problems such as limited selectivity and low sensitivity.

Method used

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  • A kind of surface polymerized ionic liquid modified magnetic nanomaterial and its preparation method and application
  • A kind of surface polymerized ionic liquid modified magnetic nanomaterial and its preparation method and application
  • A kind of surface polymerized ionic liquid modified magnetic nanomaterial and its preparation method and application

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Embodiment 1

[0055] Example 1. Preparation of surface-polymerized ionic liquid-modified magnetic nanomaterials and enrichment and detection of glycopeptides

[0056] 1. Preparation of magnetic nanomaterials with surface polymerized ionic liquids

[0057] according to figure 1 The schematic diagram of the synthesis route shown in the preparation of the surface polymerized ionic liquid modified magnetic nanomaterials, the specific steps are as follows:

[0058] Step 1: Preparation of silica-coated magnetic nanoparticles

[0059] 200mg of magnetic nanoparticles (γ-Fe 3 o 4 , particle size 150 ~ 200nm) ultrasonically dispersed in the mixture of 200mL ethanol, 50mL water and 1.5mL concentrated ammonia water (28wt%), stirred mechanically at room temperature for 30min, added 0.5mL tetraethyl orthosilicate (TEOS) dropwise, continued The reaction was stirred for 12h. The resulting product (Fe 3 o 4 @SiO 2 ) were washed three times with ethanol and water, respectively.

[0060] Step 2: Usin...

Embodiment 2

[0073] Example 2. Sensitivity detection of glycopeptide enrichment by magnetic nanomaterials with surface polymerized ionic liquids

[0074] Weigh 1 mg of magnetic nanomaterials with surface-polymerized ionic liquids, ultrasonically disperse them in 1 mL of enrichment buffer (anhydrous acetonitrile / water / trifluoroacetic acid, 88:11.9:0.1, v / v / v), and then pipette 20 μL of the suspension The turbid liquid, the magnetic nanoparticles were separated by applying an external magnetic field, and washed three times with enrichment buffer (anhydrous acetonitrile / water / trifluoroacetic acid, 88:11.9:0.1, v / v / v).

[0075]The above magnetic nanoparticles (20 μg) were resuspended in 50 μL enrichment buffer (anhydrous acetonitrile / water / trifluoroacetic acid, 88:11.9:0.1, v / v / v), and 1 μL (50 pmol / mL or 10pmol / mL) human immunoglobulin enzymatic hydrolysis solution, shake and mix at room temperature for 30min, then under the action of an external magnetic field, separate the magnetic nanopart...

Embodiment 3

[0077] Example 3. Selective detection of glycopeptide enrichment by magnetic nanomaterials with surface polymerized ionic liquids

[0078] Weigh 1 mg of magnetic nanomaterials with surface-polymerized ionic liquids, ultrasonically disperse them in 1 mL of enrichment buffer (anhydrous acetonitrile / water / trifluoroacetic acid, 88:11.9:0.1, v / v / v), and then pipette 20 μL of the suspension The turbid liquid, the magnetic nanoparticles were separated by applying an external magnetic field, and washed three times with enrichment buffer (anhydrous acetonitrile / water / trifluoroacetic acid, 88:11.9:0.1, v / v / v).

[0079] Resuspend the above-mentioned magnetic nanoparticles (20 μg) in 50 μL enrichment buffer (anhydrous acetonitrile / water / trifluoroacetic acid, 88:11.9:0.1, v / v / v), add 1 μL human immunoglobulin for enzymatic digestion solution and bovine serum albumin enzymatic hydrolysis solution mixture (IgG:BSA 1:100, w / w, 1μg / μL), shake and mix at room temperature for 30min, and then sep...

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Abstract

The invention discloses a surface polymerized ionic liquid modified magnetic nanometer material as well as a preparation method and application thereof. The surface polymerized ionic liquid modified magnetic nanometer material is prepared from magnetic nanoparticles, a silicon dioxide layer cladding the surfaces of the magnetic nanoparticles and a hydrophilic ionic liquid layer which is polymerized on the surface of the silicon dioxide layer through a mode of modifying a silane coupling agent with carbon-carbon double bonds at terminal on the surface of the silicon dioxide layer for precipitation polymerization. With adoption of the surface polymerized ionic liquid modified magnetic nanometer material, through a mode of precipitation polymerization, the ionic liquid is polymerized on the surfaces of the magnetic nanoparticles; a material synthetic method is simple, clean and rapid; through the hydrophilic interaction and electrostatic interaction of the polymerized ionic liquid and glycopeptide, efficient selective enrichment of the glycopeptide is realized; the glycopeptide enrichment effect is good, and glycopeptide enrichment detection in human serum albumin (such as IgG) with low concentration can be realized.

Description

technical field [0001] The invention belongs to the technical field of functionalized magnetic nanometer materials, in particular to a magnetic nanometer material modified by surface polymerized ionic liquid and its preparation method and application. Background technique [0002] Protein glycosylation is a common post-translational modification of proteins, which is closely related to various life activities. The current research methods for glycoproteins are mainly to cut glycoproteins into peptides first, and then analyze them by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) or liquid chromatography tandem mass spectrometry (LC-MS / MS). Due to the low content of glycoproteins in complex biological samples, signal suppression and interference of high-abundance non-glycosylated peptides also exist in mass spectrometry analysis. Therefore, the selective enrichment and separation of glycopeptides is the key to glycoprotein researc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F299/00B01J20/26B01J20/28G01N1/40B01J20/30B01D15/08
Inventor 钱小红张养军焦丰龙高方园王和平吕雅瑶吴琼夏朝双余谦
Owner ACADEMY OF MILITARY MEDICAL SCI
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