Method for enzymatic synthesis of 1,2-diacylglycerol and method for purifying obtained 1,2-diacylglycerol

A technology for diglyceride and enzymatic synthesis, which is applied in chemical instruments and methods, preparation of organic compounds, organic chemistry, etc., can solve problems such as poor thermal stability, and achieve the effect of reducing difficulty, mild conditions, and mild reaction conditions

Active Publication Date: 2017-02-15
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the poor thermal stability of 1,2-diglycerides, there is currently no professional manufacturer of 1,2-diglycerides in China

Method used

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  • Method for enzymatic synthesis of 1,2-diacylglycerol and method for purifying obtained 1,2-diacylglycerol
  • Method for enzymatic synthesis of 1,2-diacylglycerol and method for purifying obtained 1,2-diacylglycerol
  • Method for enzymatic synthesis of 1,2-diacylglycerol and method for purifying obtained 1,2-diacylglycerol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Add high oleic sunflower oil and n-propanol into the reaction kettle at a molar ratio of 1:30, put a stirring bar, preheat in a constant temperature water bath at a temperature of 40°C, turn on the magnetic stirrer, and stir at a speed of 500rpm After emulsifying for 15 minutes, add lipase Lipozyme RM IM, which accounts for 6% of the total mass of the substrate, to start the reaction. After 1 hour of reaction, the product is taken out, and the lipase is removed by centrifugation at 4000 rpm for 10 minutes. After HPLC analysis, the content of 1,2-diglyceride in the product is The yield was 45.3%.

Embodiment 2

[0042] Add high oleic sunflower oil and absolute ethanol into the reaction kettle at a molar ratio of 1:50, put a stirring bar, preheat it in a constant temperature water bath at a temperature of 50°C, turn on the magnetic stirrer, and set it at a speed of 500rpm After stirring and emulsifying for 15 minutes, add lipase Lipozyme 435 accounting for 6% of the total mass of the substrate to start the reaction. After 1 hour of reaction, the product is taken out and centrifuged at 4000 rpm for 10 minutes to remove the lipase. After HPLC analysis, the content of 1,2-diglyceride in the product is The yield was 90%.

Embodiment 3

[0044] Add palm oil and absolute ethanol to the reaction kettle at a molar ratio of 1:40, put a stirring bar, preheat in a constant temperature water bath at a temperature of 45°C, turn on the magnetic stirrer, stir and emulsify at a speed of 500rpm for 15min , add lipase Lipozyme 435 accounting for 8% of the total mass of the substrate to start the reaction. After 1 hour of reaction, the product is taken out and centrifuged at 4000prm for 10 minutes to remove the lipase. According to HPLC analysis, the yield of 1,2-diglyceride in the product is 80.5 %.

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Abstract

The invention discloses a method for enzymatic synthesis of 1,2-diacylglycerol and a method for purifying obtained 1,2-diacylglycerol. The enzymatic synthesis method comprises the steps that vegetable oil and an organic solvent are mixed based on a molar ratio of 1 to 10-100; after stirring and emulsification, immobilized lipase with the mass accounting for 2 to 20% of the total mass of a substrate is added; after reaction for 0.5 to 6 hours at a temperature of 20 to 70 DEG C, centrifugal removing of lipase is carried out to obtain a product containing 1,2-diacylglycerol. The purification method comprises the step that two-step-crystallization-process-based purification is carried out on the product and two kinds of organic solvents. The purity of the purified 1,2-diacylglycerol can reach up to 100%. According to the method for synthetizing 1,2-diacylglycerol and the method for purifying the obtained 1,2-diacylglycerol provided by the invention, the reaction rate can increase obviously and the yield is high; the product is easy to purify and the purity is high. The methods conforming to a green chemistry concept have advantages of being high in efficiency, being fast in speed, and being convenient for industrial production.

Description

technical field [0001] The invention belongs to the technical field of synthesis of structural glycerides, in particular to a method for enzymatically synthesizing 1,2-diglyceride and a purification method thereof. Background technique [0002] Diacylglycerol (DAG) is a structural lipid formed by replacing a fatty acid in a triacylglycerol (TAG) with a hydroxyl group. There are mainly two isomers of 1,3-DAG and 1,2-DAG , is generally recognized as safe (GRAS) food ingredients, but also a multifunctional additive, has broad application prospects in food, medicine, chemical and other industries. [0003] Currently developed diglycerides (mainly referring to 1,3-diglycerides) preparation methods mainly contain four kinds: [0004] The first preparation method is disclosed in Japanese Patent No. 6-3438, which uses microorganisms of the genus Pseudomonas bacteria to catalyze the enzymatic hydrolysis and transesterification of solid oil and glycerin. The obtained oily product ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C07C67/52C07C69/30C11C1/04
CPCC07C67/52C11C1/045C12P7/6445C07C69/30Y02E50/10
Inventor 王小三祝雨筱王盈盈黄健花金青哲王兴国
Owner JIANGNAN UNIV
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