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Fritillariae cirrhosae bulbus polysaccharide extraction, separation and purification technology

A technology for separation and purification of fritillary fritillary, applied in the production of bulk chemicals, etc., can solve the problems of low extraction rate and low purity, and achieve good purification effect and good separation and purification effect

Active Publication Date: 2017-02-15
CHONGQING THREE GORGES MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has high extraction rate and purity, which can overcome the shortcomings of conventional methods such as low extraction rate and low purity.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Extraction of Crude Polysaccharide from Fritillaria sichuanensis

[0026] (1.1) Extraction: take the bulbs of Fritillaria chinensis, dry and pulverize until the particle size is less than 60 mesh, put the bulbs of Fritillaria chinensis after crushing into supercritical CO 2 In the extraction kettle, seal the kettle, remove the air, pass through the carrier agent to extract the extract, the extraction temperature is 47.3°C, the extraction pressure is 23.7MPa, the extraction carbon dioxide flow rate is 20-28L / h, the carrier agent is distilled water, and the dosage is 390ml / kg, extraction 2.5h;

[0027] (1.2) Decolorization: adjust the extract obtained in step (1.1) to pH 7.8 with 1mol / L NaOH, add diatomaceous earth and heat to boil for 5min to decolorize, filter under reduced pressure to obtain the filtrate, the mass ratio of diatomite added to the extract 1.5%;

[0028] (1.3) Concentration and drying: the filtrate obtained in step (1.2) was concentrated under redu...

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Abstract

The invention discloses a fritillariae cirrhosae bulbus polysaccharide extraction, separation and purification technology. As a novel supercritical CO2 extraction technology is utilized, the defects that a general method is low in extraction rate and not high in purity are overcome; quaternary ammonium salt cetyl pyridinium chloride monohydrate and fritillariae cirrhosae bulbus polysaccharide can precipitate in water solution with low ionic strength; when the ionic strength is high, precipitate can be dissolved, dissociated and released, and the purification effect is good; DEAE-Sephadex ionic exchange column chromatography is utilized to further remove neutral impurities and impurities with positive charges, and the separation and purification effect is better.

Description

technical field [0001] The invention belongs to the technical field of methods for extracting polysaccharides from plants, and in particular relates to the extraction, separation and purification process of Fritillaria sichuanensis polysaccharides. Background technique [0002] Chuan Fritillaria is a plant of the genus Fritillaria Liliaceae, a perennial herb distributed in Sichuan, Yunnan and Sichuan. Fritillaria sichuanensis has the effect of nourishing the lungs and relieving cough. At present, the research of Fritillaria sichuanensis mainly focuses on its alkaloids, and there are few studies on the extraction and pharmacology of Fritillaria sichuanensis polysaccharides. [0003] At present, the extraction and separation methods of Fritillaria polysaccharides include hot water extraction, ethanol extraction, ultrasonic extraction and Soxhlet extraction. But the extraction rate of these conventional methods is all low (about 2%), and the purity is not high (about 80%). ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/00C08B37/0003Y02P20/54
Inventor 熊书孙厚良马强李春雷李国利
Owner CHONGQING THREE GORGES MEDICAL COLLEGE
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