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Brasenia schreberi polysaccharide preparation method

A water shield and polysaccharide technology, applied in the field of water shield polysaccharide preparation, can solve the problems of water shield polysaccharide separation and purification, very few structural studies, difficult purification, high viscosity of water shield polysaccharides, etc.

Active Publication Date: 2014-04-30
山东好当家海森药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Domestic research on water shield polysaccharides mainly focuses on the optimization of the extraction process of crude water shield polysaccharides. Due to the high viscosity of water shield polysaccharides, it is difficult to purify, so there are very few domestic studies on the separation, purification and structure of water shield polysaccharides.
At present, there is no report on separation and purification of water shield polysaccharides after extraction to obtain crude polysaccharides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A preparation method of water shield polysaccharide, through the following process steps:

[0017] A, the selection and processing of raw materials Choose fresh water shield as raw material, carry out pulverization after drying, cross 200 mesh sieves, get water shield powder, for subsequent use;

[0018] B. Enzymolysis Weigh the water shield powder obtained in step (A) and add water with 8 times the weight of water shield powder, adjust the pH to 7, add papain with 3% of the weight of water shield powder, control the enzymolysis temperature at 40°C, and enzymolysis at 1.5 h, then raise the temperature to 80°C, extract for 2h; obtain the extract;

[0019] C. Filtration and concentration Filter the extract obtained in step (B) to remove impurities to obtain the filtrate; place the filtrate in a vacuum decompression concentrator, control the vacuum degree -0.1MPa, and concentrate the filtrate to the original volume 35% of the concentrated solution;

[0020] D. Remove pro...

Embodiment 2

[0025] A preparation method of water shield polysaccharide, through the following process steps:

[0026] A, the selection and processing of raw materials choose fresh water shield as raw material, carry out pulverization after drying, cross 250 mesh sieves, get water shield powder, for subsequent use;

[0027] B. Enzymolysis Weigh the water shield powder obtained in step (A), add water with 10 times the weight of water shield powder, adjust the pH value to 8, add papain with 3% mass of water shield powder, control the enzymolysis temperature at 30°C, and enzymolysis for 1h , and then raise the temperature to 85°C, extract for 1 hour; obtain the extract;

[0028] C. Filtration and concentration Filter the extract obtained in step (B) to remove impurities to obtain the filtrate; place the filtrate in a vacuum decompression concentrator, control the vacuum degree of 0MPa, and concentrate the filtrate to 40% of the original volume. %, get concentrated solution;

[0029] D, remo...

Embodiment 3

[0034] A preparation method of water shield polysaccharide, through the following process steps:

[0035] A, the selection and processing of raw materials Choose fresh water shield as raw material, carry out pulverization after drying, cross 150 mesh sieves, get water shield powder, for subsequent use;

[0036] B. Enzymolysis Weigh the water shield powder obtained in step (A), add water with 5 times the weight of water shield powder, adjust the pH value to 6, add papain with 1% mass of water shield powder, control the enzymolysis temperature at 50°C, and enzymolysis for 2 hours , and then raise the temperature to 75°C, extract for 3 hours; obtain the extract;

[0037] C. Filtration and Concentration Filter the extract obtained in step (B) to remove impurities to obtain the filtrate; place the filtrate in a vacuum decompression concentrator, control the vacuum degree -0.1MPa, and concentrate the filtrate to the original volume 30% of the concentrated solution;

[0038] D, rem...

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Abstract

The invention relates to a Brasenia schreberi polysaccharide preparation method. The preparation method comprises the following steps: drying a raw material fresh Brasenia schreberi, and crushing to obtain Brasenia schreberi powder; adding water to the Brasenia schreberi powder for dilution, adjusting the pH value of the obtained solution, adding papain, carrying out enzymatic hydrolysis treatment under controlled conditions, filtering, concentrating, adding a mixture containing organic solvents comprising chloroform and n-butanol, and stirring for removing proteins; carrying out dialysis desalting through a dialysis membrane, carrying out alcohol precipitation, collecting the obtained precipitate, washing, and drying to obtain crude Brasenia schreberi polysaccharides. The preparation method has a high protein removal rate, a small polysaccharide loss and a good separation and purification effect, and determination results show that the protein removal rate reaches above 80%, and the purity of the obtained crude Brasenia schreberi polysaccharides reaches above 70%. The preparation method has the advantages of reasonable technology, advanced technique, feasible operation, and suitableness for the large-scale factory production.

Description

technical field [0001] The invention relates to the preparation of polysaccharides, in particular to a preparation method of water shield polysaccharides. Background technique [0002] Water shield ( Brasenia schreber J. F. Gme1) is a perennial freshwater aquatic herb of the subclass Nymphaeaceae (Nymphaeaceae), also known as callagrass, lake vegetable, water anemone, and dew anemone. It is native to southeastern my country and grows in ponds, lakes, and swamps. , especially in Taihu Lake in Jiangsu, Gaobin Lake in northern Jiangsu, and West Lake in Hangzhou. [0003] The stem and leaf surface of water shield contains abundant glandular cells, which can secrete a large amount of transparent gelatin. The water content of fresh water shield samples is as high as 97.5%, and the total sugar content is 0.86%. Among them, water shield polysaccharides are mainly present in the transparent mucus on the stems and leaves of water shield. The research on the biological activity of...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 刘新刘昌衡胡炜齐君乔若瑾张绵松袁文鹏孙永军贾爱荣夏雪奎张永刚
Owner 山东好当家海森药业有限公司
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