The invention relates to a Brasenia schreberi
polysaccharide preparation method. The preparation method comprises the following steps:
drying a
raw material fresh Brasenia schreberi, and crushing to obtain Brasenia schreberi
powder; adding water to the Brasenia schreberi
powder for
dilution, adjusting the pH value of the obtained solution, adding
papain, carrying out
enzymatic hydrolysis treatment under controlled conditions, filtering, concentrating, adding a mixture containing organic solvents comprising
chloroform and n-
butanol, and stirring for removing proteins; carrying out
dialysis desalting through a
dialysis membrane, carrying out
alcohol precipitation, collecting the obtained precipitate, washing, and
drying to obtain crude Brasenia schreberi polysaccharides. The preparation method has a
high protein removal rate, a small
polysaccharide loss and a good separation and purification effect, and determination results show that the
protein removal rate reaches above 80%, and the purity of the obtained crude Brasenia schreberi polysaccharides reaches above 70%. The preparation method has the advantages of reasonable technology, advanced technique, feasible operation, and suitableness for the large-scale factory production.