Saccharomyces cerevisiae strain and high quality red bayberry fruit wine preparation method
A Saccharomyces cerevisiae strain and high-quality technology, applied in the field of microbial fermentation, can solve the problems of inhibition of fermentation performance, loss of color of anthocyanins, less color recovery, etc., and achieve the effects of fast fermentation, high retention rate and rich fruit aroma.
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Embodiment 1
[0027] Embodiment 1: Breeding process of Saccharomyces cerevisiae strain CCTCCNO:M2015627:
[0028] (1) Configuration of bayberry juice fermentation medium: fresh red bayberry fruit was pitted and squeezed, filtered with sterilized four-layer gauze, and centrifuged at 5000r / min for 5min in a centrifuge. Add sucrose to the clarified fruit juice to adjust the TSS to 20°Brix, the pH to the natural pH value of bayberry juice 3.0, and the liquid volume to be 3 / 5 of the volume of the fermentation bottle, sterilized at 100°C for 5 minutes, and then cooled rapidly for later use.
[0029] (2) Separation of saccharomycetes: After commercially available fresh red bayberry is crushed and denuclearized, it is put into a sterilized Erlenmeyer flask, added 10% (v / v) ethanol, shaken up and wrapped with 4 layers of gauze, at 28 ℃ static fermentation, after a large number of bubbles appear, replace the fermentation plug. Take 5 mL of the fermentation broth at the end of the fermentation, add i...
Embodiment 2
[0048] Embodiment 2: the method for preparing high-quality bayberry fruit wine with Saccharomyces cerevisiae strain CCTCCNO:M2015627:
[0049] (1) Raw material processing: select the bayberry fruit with high maturity, no rot, and darker color as raw material, remove the nucleus and squeeze the juice after cleaning, filter with sterilized four-layer gauze, and then centrifuge at 5000r / min for 5min in a centrifuge. Add sucrose to the clarified fruit juice to adjust TSS to 22°Brix, pH to 3.0 (the natural pH of bayberry juice), the liquid volume is 3 / 5 of the volume of the fermentation bottle, sterilized at 100°C for 5 minutes, and then cooled rapidly for later use.
[0050] (2) Strain activation: Pick the Saccharomyces cerevisiae strain CCTCCNO:M2015627 from the YPD slant onto a fresh YPD plate, culture it in a biochemical incubator at 28°C for 24 hours, and then use an inoculation loop to pick 3 loops into 50mL YPD liquid medium , 28°C, 150rmp shaker culture for 12h, inoculated ...
Embodiment 3
[0059] Embodiment 3: the method for preparing high-quality bayberry fruit wine with Saccharomyces cerevisiae strain CCTCCNO:M2015627:
[0060] (1) Raw material processing: select the bayberry fruit with high maturity, no rot, and darker color as raw material, remove the nucleus and squeeze the juice after cleaning, filter with sterilized four-layer gauze, and then centrifuge at 4500r / min for 6min in a centrifuge. Add sucrose to the clarified fruit juice to adjust the TSS to 20°Brix, pH to 2.5, the liquid volume is 3 / 5 of the volume of the fermentation bottle, sterilized at 90°C for 7 minutes, then cooled rapidly for later use.
[0061](2) Strain activation: Pick the Saccharomyces cerevisiae strain CCTCCNO:M2015627 from the YPD slant onto a fresh YPD plate, culture it in a biochemical incubator at 28°C for 24 hours, and then use an inoculation loop to pick 3 loops into 50mL YPD liquid medium , 28°C, 150rmp shaker culture for 12h, inoculated into 100mLYPD liquid medium according...
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