A Saccharomyces cerevisiae strain and the method for preparing red bayberry wine

A technology for Saccharomyces cerevisiae strain and fruit wine, which is applied in the field of microbial fermentation, can solve the problems of less color recovery, anthocyanin discoloration, and red color is far away, and achieves the effects of fast fermentation speed, high alcohol production, and good tolerance.

Active Publication Date: 2018-10-16
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There are two kinds of red bayberry wine in China at present, and a kind of is the red bayberry soaked wine made by farmers, the alcohol content is too high (about 50%), it is not suitable for regular drinking; They are mainly developed for wine and lack of pertinence to red bayberry. The pH value used when brewing bayberry wine is relatively high (generally around 3.5, and the optimum fermentation pH is above 4.0), while the pH of red bayberry juice is generally around 3.0 , when adjusting the fermentation pH to be 3.5, the stability of red bayberry anthocyanins will become poor, causing a large amount of loss of red bayberry anthocyanins in the brewing process, and the color of the product is light red, which is far from the bright red color of red bayberry juice; and the fermentation process Added SO 2 (>50mg / L), not only delays the fermentation time of the yeast, but also has a bleaching effect, resulting in a lighter color of the product, even if the method of heating is finally used to dissolve the SO 2 removed, but the color recovery is less, orange-red; yeast will produce a kind of β-D-glucosidase during the fermentation process, which will desugar the colored anthocyanins in bayberry juice, resulting in color loss or degradation of anthocyanins, and Most commercial yeast or wine active dry yeast have inhibited fermentation performance at pH 3.0, and the product alcohol content is less than 10% (v / v)

Method used

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  • A Saccharomyces cerevisiae strain and the method for preparing red bayberry wine
  • A Saccharomyces cerevisiae strain and the method for preparing red bayberry wine
  • A Saccharomyces cerevisiae strain and the method for preparing red bayberry wine

Examples

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Embodiment 1

[0027] Embodiment 1: Breeding process of Saccharomyces cerevisiae strain CCTCC NO:M 2015627:

[0028](1) Configuration of bayberry juice fermentation medium: fresh red bayberry fruit was pitted and squeezed, filtered with sterilized four-layer gauze, and centrifuged at 5000r / min for 5min in a centrifuge. Add sucrose to the clarified fruit juice to adjust the TSS to 20°Brix, the pH to the natural pH value of bayberry juice 3.0, and the liquid volume to be 3 / 5 of the volume of the fermentation bottle, sterilized at 100°C for 5 minutes, and then cooled rapidly for later use.

[0029] (2) Separation of saccharomycetes: After crushing and removing the nucleus of commercially available fresh red bayberry, put it into a sterilized Erlenmeyer flask, add 10% (v / v) ethanol, shake it up, wrap it with 4 layers of gauze, and put it in 28 ℃ static fermentation, after a large number of bubbles appear, replace the fermentation plug. Take 5 mL of the fermentation broth at the end of the ferme...

Embodiment 2

[0047] Embodiment 2: the method for preparing bayberry fruit wine with Saccharomyces cerevisiae strain CCTCC NO:M 2015627:

[0048] (1) Raw material processing: select the bayberry fruit with high maturity, no rot, and darker color as raw material, remove the nucleus and squeeze the juice after cleaning, filter with sterilized four-layer gauze, and then centrifuge at 5000r / min for 5min in a centrifuge. Add sucrose to the clarified fruit juice to adjust TSS to 22°Brix, pH to 3.0 (the natural pH of bayberry juice), the liquid volume is 3 / 5 of the volume of the fermentation bottle, sterilized at 100°C for 5 minutes, and then cooled rapidly for later use.

[0049] (2) Strain activation: Pick Saccharomyces cerevisiae strain CCTCC NO:M 2015627 from the YPD slant onto a fresh YPD plate, culture it in a biochemical incubator at 28°C for 24 hours, and pick 3 loops to 50mL of YPD liquid with an inoculation loop In the culture medium, cultivate it on a shaker at 28°C and 150rmp for 12h, ...

Embodiment 3

[0057] Embodiment 3: the method for preparing bayberry fruit wine with Saccharomyces cerevisiae strain CCTCC NO:M 2015627:

[0058] (1) Raw material processing: select the bayberry fruit with high maturity, no rot, and darker color as raw material, remove the nucleus and squeeze the juice after cleaning, filter with sterilized four-layer gauze, and then centrifuge at 4500r / min for 6min in a centrifuge. Add sucrose to the clarified fruit juice to adjust the TSS to 20°Brix, pH to 2.5, the liquid volume is 3 / 5 of the volume of the fermentation bottle, sterilized at 90°C for 7 minutes, then cooled rapidly for later use.

[0059] (2) Strain activation: Pick Saccharomyces cerevisiae strain CCTCC NO:M 2015627 from the YPD slant onto a fresh YPD plate, culture it in a biochemical incubator at 28°C for 24 hours, and pick 3 loops to 50mL of YPD liquid with an inoculation loop In the culture medium, cultivate it on a shaker at 28°C and 150rmp for 12h, inoculate it into 100mL YPD liquid m...

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Abstract

The present invention discloses a saccharomyces cerevisiae strain and a high quality red bayberry fruit wine preparation method. The saccharomyces cerevisiae strain is screened from naturally fermented mash of fresh red bayberry, red bayberry juice with pH of 3.0 can be directly fermented to obtain high quality red bayberry fruit wine with high alcohol content (13.5%), bright color (a = 27.64), high anthocyanin retention rate (77mg / L), low residual sugar (< 2g / L), rich fruity taste and harmonious taste, the saccharomyces cerevisiae strain is preserved in China Type Culture Collection CCTCC on October 20, 2015, and the accession number is CCTCC NO:M2015627. The high quality red bayberry fruit wine is prepared by the following steps: raw material processing, bacteria activation, main fermentation and post fermentation. In the fermentation process, no SO2 is added, and by sterilization for 5min at 100 DEG C, the method has a very significant effect on color and anthocyanin retention rate of the fermented red bayberry fruit wine.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a strain of Saccharomyces cerevisiae and a method for preparing bayberry fruit wine. Background technique [0002] Red bayberry (Red bayberry) is a characteristic fruit in southern my country. Its fruit is spherical, bright in color, sweet and sour, juicy, rich in flavor, and the edible rate reaches more than 90%. It is rich in sugar, organic acids and multivitamins, and also contains anthocyanins. , polyphenols and minerals such as calcium, phosphorus, iron, potassium, etc., have high nutritional and medical care value. The consumption method of red bayberry is mainly fresh food, because it is abundant in the plum rain season and the storage period is short, resulting in a large backlog and rot. Vigorously developing the production of fruit wine can not only reduce food consumption, but also improve the structure of alcohol consumption, meet consumer d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C12G3/02C12R1/865
Inventor 范柳萍杜晶李洁莹
Owner JIANGNAN UNIV
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