A Saccharomyces cerevisiae strain and the method for preparing red bayberry wine
A technology for Saccharomyces cerevisiae strain and fruit wine, which is applied in the field of microbial fermentation, can solve the problems of less color recovery, anthocyanin discoloration, and red color is far away, and achieves the effects of fast fermentation speed, high alcohol production, and good tolerance.
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Embodiment 1
[0027] Embodiment 1: Breeding process of Saccharomyces cerevisiae strain CCTCC NO:M 2015627:
[0028](1) Configuration of bayberry juice fermentation medium: fresh red bayberry fruit was pitted and squeezed, filtered with sterilized four-layer gauze, and centrifuged at 5000r / min for 5min in a centrifuge. Add sucrose to the clarified fruit juice to adjust the TSS to 20°Brix, the pH to the natural pH value of bayberry juice 3.0, and the liquid volume to be 3 / 5 of the volume of the fermentation bottle, sterilized at 100°C for 5 minutes, and then cooled rapidly for later use.
[0029] (2) Separation of saccharomycetes: After crushing and removing the nucleus of commercially available fresh red bayberry, put it into a sterilized Erlenmeyer flask, add 10% (v / v) ethanol, shake it up, wrap it with 4 layers of gauze, and put it in 28 ℃ static fermentation, after a large number of bubbles appear, replace the fermentation plug. Take 5 mL of the fermentation broth at the end of the ferme...
Embodiment 2
[0047] Embodiment 2: the method for preparing bayberry fruit wine with Saccharomyces cerevisiae strain CCTCC NO:M 2015627:
[0048] (1) Raw material processing: select the bayberry fruit with high maturity, no rot, and darker color as raw material, remove the nucleus and squeeze the juice after cleaning, filter with sterilized four-layer gauze, and then centrifuge at 5000r / min for 5min in a centrifuge. Add sucrose to the clarified fruit juice to adjust TSS to 22°Brix, pH to 3.0 (the natural pH of bayberry juice), the liquid volume is 3 / 5 of the volume of the fermentation bottle, sterilized at 100°C for 5 minutes, and then cooled rapidly for later use.
[0049] (2) Strain activation: Pick Saccharomyces cerevisiae strain CCTCC NO:M 2015627 from the YPD slant onto a fresh YPD plate, culture it in a biochemical incubator at 28°C for 24 hours, and pick 3 loops to 50mL of YPD liquid with an inoculation loop In the culture medium, cultivate it on a shaker at 28°C and 150rmp for 12h, ...
Embodiment 3
[0057] Embodiment 3: the method for preparing bayberry fruit wine with Saccharomyces cerevisiae strain CCTCC NO:M 2015627:
[0058] (1) Raw material processing: select the bayberry fruit with high maturity, no rot, and darker color as raw material, remove the nucleus and squeeze the juice after cleaning, filter with sterilized four-layer gauze, and then centrifuge at 4500r / min for 6min in a centrifuge. Add sucrose to the clarified fruit juice to adjust the TSS to 20°Brix, pH to 2.5, the liquid volume is 3 / 5 of the volume of the fermentation bottle, sterilized at 90°C for 7 minutes, then cooled rapidly for later use.
[0059] (2) Strain activation: Pick Saccharomyces cerevisiae strain CCTCC NO:M 2015627 from the YPD slant onto a fresh YPD plate, culture it in a biochemical incubator at 28°C for 24 hours, and pick 3 loops to 50mL of YPD liquid with an inoculation loop In the culture medium, cultivate it on a shaker at 28°C and 150rmp for 12h, inoculate it into 100mL YPD liquid m...
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