Paecilomyces fumosoroseus oil, preparation method of paecilomyces fumosoroseus oil and insecticide

A technology of Paecilomyces rosaceum and Penicillium oil, which is applied in the fields of pesticides, botanical equipment and methods, biocides, etc., can solve the problems of short survival time, death and failure of Paecilomyces fumigatus, etc.

Active Publication Date: 2017-12-01
INST OF MICROBIOLOGY JIANGXI ACADEMY OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After infecting the host, the pathogenic fungus has strong pathogenicity and a long validity period. The fungus has strong infectivity to the broad-bellied mantis and various pine caterpillars, and has a strong aphidicidal effect, but the rose smoke color is similar to The effective insecticidal substance of Penicillium is live spores of the fungus, which has high requirements on the environment. Unsuitable environment can easily cause the natural death and failure of Paecilomyces fumigatus, resulting in a short survival time

Method used

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  • Paecilomyces fumosoroseus oil, preparation method of paecilomyces fumosoroseus oil and insecticide
  • Paecilomyces fumosoroseus oil, preparation method of paecilomyces fumosoroseus oil and insecticide
  • Paecilomyces fumosoroseus oil, preparation method of paecilomyces fumosoroseus oil and insecticide

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preparation example Construction

[0026] In the present invention, the preparation method of Paecilomyces fumigatus comprises the following steps:

[0027] 1) activating the Paecilomyces fumigatus species;

[0028] 2) inoculating the activated Paecilomyces fumigatus obtained in step 1) in the primary liquid culture medium of Paecilomyces fumigatus to carry out primary liquid culture to obtain the primary seed liquid of Paecilomyces fumigatus ;

[0029] 3) inoculating the primary seed liquid obtained in step 2) in the secondary liquid culture medium of Paecilomyces fumigatus for secondary liquid culture to obtain the secondary seed liquid of Paecilomyces fumigatus;

[0030] 4) inoculating the secondary seed solution obtained in step 3) into the solid medium of Paecilomyces fumigatus, and performing solid culture to obtain Paecilomyces fumigatus.

[0031] In the present invention, the activation of the Paecilomyces fumigatus strains is carried out by inoculating the preserved Paecilomyces fumigatus strains in ...

Embodiment 1

[0070] Preparation of the primary seed liquid of Paecilomyces fumigatus: Inoculate the preserved Paecilomyces fumigatus strains into eggplant-shaped bottles equipped with nutrient agar medium, and cultivate them at 28°C for 12 hours for activation culture to complete the activation of the strains; The Paecilomyces fumigatus strain after activation is inoculated in the first-level liquid culture medium of Paecilomyces fumigatus according to the inoculation amount of 1%, and the first-level liquid culture medium comprises the component of following mass content: potato flour 33g / L, White sugar 20g / L, peptone 1.0g / L, sodium nitrate 0.1g / L, potassium chloride 0.25g / L, magnesium sulfate 0.05g / L, iron sulfate 0.05g / L, manganese sulfate 0.05g / L, hydrogen phosphate Dipotassium 0.5g / L, the balance is water. The temperature of the primary liquid culture of Paecilomyces fumigatus is 25°C, and the initial pH value is 6.5. 3 / h ventilation for aeration culture, after 24 hours of liquid cu...

Embodiment 2

[0075] Preparation of Paecilomyces fumigatus oil agent: based on the total amount of Paecilomyces fumigatus oil agent, the Paecilomyces fumigatus obtained in Example 1, 10% diatomaceous earth, 10% silica gel, 2% phenylalanine, 2% salicyl phenyl ester, 3% dibutyl hydroxytoluene, 3% tert-butyl hydroquinone are mixed and pulverized to obtain a mixture containing Paecilomyces fumigatus ; Mix 20% soybean salad oil, 5% fatty acid sorbitan, 5% glyceryl caprylate, 5% sodium hexametaphosphate, and 5% sodium pyrophosphate to obtain a mixture containing a dispersant; The mold mixture is mixed with the mixture containing the dispersant to obtain Paecilomyces fumigatus oil.

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Abstract

The invention provides paecilomyces fumosoroseus oil which comprises the following components in percentage by mass: 30-50% of paecilomyces fumosoroseus, 15-40% of a solvent, 2-20% of an emulsifier, 2-40% of an adsorbent, 1-10% of an ultraviolet absorbent, 1-10% of an antioxidant and 2-20% of a dispersing agent. The paecilomyces fumosoroseus oil prepared by the invention is respectively stored in environments at the temperature of 4 DEG C and 25 DEG C, the germination rate is determined every six months, and the germination rate within 24 months is respectively 90.4% and 82.9%. The paecilomyces fumosoroseus oil prepared by the invention has an insect dropping rate of 69.1-86.0%.

Description

technical field [0001] The invention belongs to the technical field of pesticide production, and in particular relates to a Paecilomyces fumigatus oil agent, a preparation method thereof and an insecticide. Background technique [0002] Paecilomyces fumosoroseus is a pathogenic fungus with a wide host range, and its hosts include different stages of various insects such as Lepidoptera, Diptera, Coleoptera, Hymenoptera, and Hemiptera. After infecting the host, the pathogenic fungus has strong pathogenicity and a long validity period. The fungus has strong infectivity to the broad-bellied mantis and various pine caterpillars, and has a strong aphidicidal effect, but the rose smoke color is similar to The effective insecticidal substance of Penicillium is live spores of the fungus, which has high requirements on the environment, and an unsuitable environment can easily cause Paecilomyces fumigatus to die naturally and fail, resulting in a short survival time. Contents of the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01N43/40A01N25/04A01P7/04
CPCA01N25/04A01N43/40A01N63/30
Inventor 郑国华靳亮孙然白娅妮梁小文李肖宇陈玉芹吴俊杰石仕福罗剑辉王成龙
Owner INST OF MICROBIOLOGY JIANGXI ACADEMY OF SCI
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