Method for improving biological control efficacy of antagonistic yeasts for postharvest acidic pathogenic fungi of fruits and vegetables and application

A technology for antagonizing yeast and pathogenic fungi, which is applied in the field of improving the effectiveness of acidic pathogenic fungi antagonizing yeast biological control after harvesting fruits and vegetables, can solve problems such as prolonging the lagging period, restricting development, stressing yeast growth, etc., and achieves reduced damage, low cost, and enhanced effect of ability

Active Publication Date: 2021-01-01
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, low concentrations of organic acids have been shown to still stress yeast growth, etc., inhibiting their growth, resulting in a prolonged lag phase
[0005] At present, there are few studies on how to enhance the stable biological control effect of antagonistic yeasts on acidic fruit wounds, which seriously limits the development of technology to improve the activity of fruit biocontrol yeasts
At present, the methods to improve the acid resistance and biological activity of yeast are mainly focused on the transformation of genetically engineered bacteria, and combined with the toxic factors of acid pathogenic fungi (oxalic acid, citric acid and glucuronic acid, etc.), the fermentation process optimization method can effectively enhance the antagonistic yeast. Research on the effectiveness of biological control has not been reported

Method used

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  • Method for improving biological control efficacy of antagonistic yeasts for postharvest acidic pathogenic fungi of fruits and vegetables and application
  • Method for improving biological control efficacy of antagonistic yeasts for postharvest acidic pathogenic fungi of fruits and vegetables and application
  • Method for improving biological control efficacy of antagonistic yeasts for postharvest acidic pathogenic fungi of fruits and vegetables and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] A method for improving the biological control effect of acidic pathogenic fungi against yeasts after harvesting fruits and vegetables, the steps of the method are as follows:

[0050] ⑴Activate biocontrol yeast;

[0051] (2) Liquid culture the activated biocontrol yeast;

[0052] The biocontrol yeast is marine yeast Rhodosporidium paludigenum Fell&Tallman, and the strain preservation number is IMI 394084.

[0053] And, in step (1), the activation medium is:

[0054] 200 grams of potatoes,

[0055] Glucose or fructose 0 g,

[0056] Fumaric acid 1-100mmol L -1 ,

[0057] 20 grams of agarose,

[0058] 1 liter of tap water was used to obtain an activated medium after sterilization.

[0059] And, in step (2), the liquid culture medium is:

[0060] 200 grams of potatoes,

[0061] Glucose or fructose 0 g,

[0062] Fumaric acid 1-100mmol L -1 ,

[0063] The volume of tap water was adjusted to 1L, and liquid culture was obtained after sterilization.

[0064] Moreove...

Embodiment 2

[0067] A method for improving the biological control effect of acidic pathogenic fungi against yeasts after harvesting fruits and vegetables, the steps of the method are as follows:

[0068] ⑴Activate biocontrol yeast;

[0069] (2) Liquid culture the activated biocontrol yeast;

[0070] The biocontrol yeast is marine yeast Rhodosporidium paludigenum Fell&Tallman, and the strain preservation number is IMI 394084.

[0071] And, in step (1), the activation medium is:

[0072] 200 grams of potatoes,

[0073] Glucose or fructose 20 grams,

[0074] Malic acid 1-100mmol L -1 ,

[0075] 20 grams of agarose,

[0076] 1 liter of tap water was used to obtain an activated medium after sterilization.

[0077] And, in step (2), the liquid culture medium is:

[0078] 200 grams of potatoes,

[0079] Glucose or fructose 20 grams,

[0080] Malic acid 1-100mmol L -1 ,

[0081] The volume of tap water was adjusted to 1L, and liquid culture was obtained after sterilization.

[0082] M...

Embodiment 3

[0085] A method for improving the biological control effect of acidic pathogenic fungi against yeasts after harvesting fruits and vegetables, the steps of the method are as follows:

[0086] ⑴Activate biocontrol yeast;

[0087] (2) Liquid culture the activated biocontrol yeast;

[0088] The biocontrol yeast is marine yeast Rhodosporidium paludigenum Fell&Tallman, and the strain preservation number is IMI 394084.

[0089] And, in step (1), the activation medium is:

[0090] 200 grams of potatoes,

[0091] 15 grams of glucose or fructose,

[0092] Glucuronic acid 200mmol L -1 ,

[0093] 20 grams of agarose,

[0094] 1 liter of tap water was used to obtain an activated medium after sterilization.

[0095] And, in step (2), the liquid culture medium is:

[0096] 200 grams of potatoes,

[0097] 15 grams of glucose or fructose,

[0098] Glucuronic acid 200mmol L -1 ,

[0099] The volume of tap water was adjusted to 1L, and liquid culture was obtained after sterilization....

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Abstract

The invention relates to a method for improving the biological control efficacy of antagonistic yeasts for postharvest acidic pathogenic fungi of fruits and vegetables. The method comprises the following steps of (1) activating biocontrol yeasts; and (2) carrying out liquid culture on the activated biocontrol yeasts. The biocontrol yeasts are marine yeasts Rhodosporidium paludigenum Fell&Tallman, and a strain preservation number is IMI 394084; organic acid is added into a culture medium, so that the capacity of the antagonistic yeasts in preventing and controlling the postharvest acidic pathogenic fungi of the fruits and vegetables is improved; the acidic pathogenic fungi include penicillium digitatum, penicillium italicum, penicillium expansum, botrytis cinerea and sclerotinia; and the method can effectively improve the survival rate of the antagonistic yeasts in different organic acid stresses, improves the capacity of the antagonistic yeasts in preventing and controlling postharvest diseases, is safe, efficient, green and pollution-free, conforms to the natural and environment-friendly concept, and is easy for large-scale application due to the high growth and propagation speed of microorganisms.

Description

technical field [0001] The invention belongs to the field of postharvest disease prevention and control of fruits and vegetables, and relates to antagonistic yeast biological control technology, in particular to a method and application for improving the effectiveness of postharvest acidic pathogenic fungi antagonistic yeast biological control of fruits and vegetables. Background technique [0002] Postharvest diseases of fruits and vegetables are mainly caused by pathogenic fungi, which cause the loss of fruit and vegetable products in my country to reach tens of billions of yuan every year, and cause serious resource and economic losses. Pathogenic fungi will not only cause huge losses in fruit quantity, but also a large number of secondary metabolites secreted by many pathogenic fungi may cause serious food safety problems, such as patulin produced by Penicillium expanses. [0003] The pathogenic fungi that cause postharvest diseases of fruits and vegetables mainly includ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12N1/38A23B7/155C12R1/645
CPCC12N1/16C12N1/38A23B7/155Y02A50/30
Inventor 路来风张泽华张浩然范春阳孙久云杨莹孙丹丹
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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