A kind of isolation method and application of marine bacillus amyloliquefaciens 1a

Abstract

The invention discloses antibacterial and anti-virus applications of marine bacillus amyloliquefaciens 1A in fruit postpartum pathogenic fungi. The fruit postpartum pathogenic fungi are colletotrichum gloeosporioides, coniella diplodiella, pear alternaria alternata and penicillium italicum. The separating method of the marine bacillus amyloliquefaciens 1A comprises the following steps: collecting seawater, sea mud, marine debris, clam, enteromorpha and mudskipper samples in Lianyungang sea area; carrying out separation and purification of bacteria after enriching, so as to obtain a plurality of pure strains of marine bacteria; determining the inhibiting effects of the pure strains of the marine bacteria on the four fruit postpartum pathogenic fungi for a test by using a plate confrontation method; screening to obtain the strains of which the width of the bacteriostatic band is greater than 5mm; determining the bacteriostatic action of sterile fermented liquid on the four fruit postpartum pathogenic fungi by using an oxford cup method; and screening to obtain the bacillus amyloliquefaciens 1A of which the width of the bacteriostatic band is greater than 10mm. Therefore, the marine bacillus amyloliquefaciens 1A has a broad application prospect.

Description

technical field [0001] The present invention relates to a method for isolating bacterial strains, in particular to a method for isolating marine Bacillus amyloliquefaciens 1A, and the present invention also relates to the application of Bacillus amyloliquefaciens 1A obtained by the aforementioned separation method. Background technique [0002] Fruit postharvest rot is a global problem. Fresh fruits may be rotted and lost during the whole process of harvesting, grading, packaging, transportation, storage, wholesale, and retailing. About 25% of the fresh fruits and vegetables are stored and transported worldwide and cannot be used due to rot. , Some perishable fruits have a rotten loss of more than 30% after harvest. At present, the control of post-harvest diseases of fruit mainly relies on chemical control. However, problems such as pesticide residues in agricultural products, pathogenic bacteria resistance, and environmental pollution caused by the extensive use of chemica...

AI Technical Summary

Problems solved by technology

At present, the control of post-harvest diseases of fruit mainly depends on chemical control. However, problems such as pesticide residues in agricultural products, pathogenic bacteria resistance, and environmental pollution caused by the extensive use of chemical pesticides have become worldwide problems.

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