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Method for extracting pinocembrin-7-O-belta-D-glucoside from hairy fig and application of pinocembrin-7-O-belta-D-glucoside

A technology of five-finger peach fruit and glucoside, which is applied in the field of botanical pesticides, and achieves the effect of simple operation steps and less usage

Inactive Publication Date: 2016-08-03
JIANGXI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Pinocetin-7-O-β-D-glucoside is a kind of flavonoids, and there are few reports on the isolation of this compound from plants. , Eagle tea, roses, green bark wood, litchi core, licorice glabra and Cyclocarya paliurus

Method used

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  • Method for extracting pinocembrin-7-O-belta-D-glucoside from hairy fig and application of pinocembrin-7-O-belta-D-glucoside
  • Method for extracting pinocembrin-7-O-belta-D-glucoside from hairy fig and application of pinocembrin-7-O-belta-D-glucoside
  • Method for extracting pinocembrin-7-O-belta-D-glucoside from hairy fig and application of pinocembrin-7-O-belta-D-glucoside

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: the preparation method of compound

[0028] Dry the peach fruit at 45°C for 2 hours in a blast drying oven, crush it with a grinder and pass it through a 40-mesh sieve, take 5.0Kg of the peach fruit powder under the sieve, soak it in 50L of ethanol solution with a volume fraction of 90%, and extract it with ultrasonic assistance 60min, the extraction temperature is 60°C, repeat three times until the color of the extract becomes light, the extracts are combined and filtered, the filtrate is concentrated at 45°C until it has no alcohol smell, and a total of 350g of extract is obtained. Suspend the extract in 4L of distilled water, go through D101 macroporous resin column chromatography, first elute with pure water until the effluent is colorless, then use 5 times column volume 30% ethanol, 5 times column volume 50% ethanol and Gradient elution with 5 times column volume of 95% ethanol, the water eluted fraction was discarded, and the other eluted fractions w...

Embodiment 2

[0030] Embodiment 2: Compound inhibits the activity of citrus postharvest Penicillium italica

[0031] 1 Materials Penicillium italicum (Penicillium italicum, ACCC30399) was purchased from China Agricultural Microorganism Culture Collection Center. Medium: PDA and PDB (PDA without agar powder) medium; cell wall degrading enzyme induction medium (KNO 3 2.0g, K 2 HPO 4 1.0g, MgSO 4 ·7H 2 O0.5g, KCl0.5g, FeSO 4 0.01g, Vitamin B 1 0.1g, L-asparagine 0.5g, chitin or dextran, CMC10.0g, H 2 (01000mL), adjust the pH to 5.0, and sterilize at 121°C for 20min after aliquoting.

[0032] 2 Experimental methods

[0033] ①Inhibitory effect of jocetin-7-O-β-D-glucoside on Penicillium italicum

[0034] The growth rate method was used to determine the inhibitory toxicity of joconin-7-O-β-D-glucoside on the growth of Penicillium italicum mycelium. From the edge of the activated Penicillium italicum colony, a bacterium cake (Ф=5mm) was obtained with a sterilized puncher, placed in the c...

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Abstract

The invention discloses a method for extracting and separating a flavonoid compound pinocembrin-7-O-belta-D-glucoside from a hairy fig and application of the pinocembrin-7-O-belta-D-glucoside and belongs to the technical field of botanical pesticides. Particularly the compound is separated with the method of subjecting a hairy fig crude extract to macroporous resin column chromatography, Sephadex LH-20 gel column chromatography and recrystallization, and the compound has good bacteriostatic activity to citrus postharvest pathogen-penicillium italicum and good disease resistance to citrus blue mold and can be used for developing a novel plant source citrus preservative.

Description

technical field [0001] The invention belongs to the technical field of botanical pesticides, and in particular relates to a method for extracting and isolating a flavonoid compound, joconin-7-O-β-D-glucoside, from the fruit of Prunus chinensis and its use in post-harvest preservation of citrus the use of. Background technique [0002] Ficus simplicissima Lour. is the fruit of Ficus simplicissima Lour., a plant of the genus Ficus spp., which has been recorded in the "Chinese Pharmacopoeia" and various ancient books on traditional Chinese medicine over the years. Wuzhi Maotao is an authentic Chinese medicinal material in Guangdong. It has a good effect of nourishing qi and invigorating the spleen, and has the reputation of "Guangdong ginseng". Five-finger peach is flat in nature, sweet and pungent, and has the functions of invigorating the spleen and nourishing the lungs, promoting dampness and relaxing tendons. It can be used for spleen deficiency and edema, lack of food and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08A23B7/154
CPCA23B7/154A23V2002/00C07H1/08C07H17/07A23V2200/10A23V2250/2116
Inventor 万春鹏陈楚英陈明陈金印
Owner JIANGXI AGRICULTURAL UNIVERSITY
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