Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of Pichia anomaly and its application

A Pichia pastoris, abnormal technology, applied in the field of microorganisms

Inactive Publication Date: 2015-08-05
北京莱万生物技术有限公司
View PDF2 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Pichia anomala is widely distributed in nature, but there is no report of Pichia anomala controlling apple gray mold, blue mold and peach brown rot in China

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of Pichia anomaly and its application
  • A kind of Pichia anomaly and its application
  • A kind of Pichia anomaly and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Isolation and strain identification of Pichia anomala YPL6 described in the present invention

[0062] 1. Sample collection

[0063] Collect fruits from organic apple orchards in China.

[0064] 2. Isolation and screening of strains and antagonistic screening

[0065] Isolate strains from organic fruits, use conventional gradient dilution coating to isolate, use two kinds of media, PDA (containing streptomycin sulfate) and YPD agar (containing streptomycin sulfate), to culture at 28°C respectively, and pick colonies Bacteria with large morphological differences and can be purely cultivated were purified and preserved on YPD agar medium, and the initial screening and multiple re-screening of antagonistic bacteria were carried out with Botrytis cinerea as the target pathogen, and finally a strain with strong antibacterial activity was obtained. Yeast strain, named YPL6.

[0066] 3. Identification of strains

[0067] Morphological, physiological and biochemical charact...

Embodiment 2

[0072] Determination of the antibacterial spectrum of Pichia anomala (Pichia anomala) YPL6 in the present invention

[0073] Pathogens tested:

[0074] Peach brown rot fungus - Monilinia fructicola (wint.) Rehm;

[0075] Botrytis cinerea Per.ex Fr, the postharvest botrytis cinerea of ​​apples;

[0076] Penicillium expansum Link, 1809, the postharvest penicillium fungus of apples;

[0077] Penicillium expansum Link, 1809, the postharvest penicillium of pears;

[0078] The above-mentioned pathogenic bacteria were selected as indicator fungi, and the bioassay method was artificially punched and inoculated in the fruit (taking the control of postharvest gray mold of apples as an example for illustration).

[0079] The specific operation is as follows:

[0080] The artificial punching inoculation method in the fruit body is as follows: the pathogenic bacteria strains were cultured on the YPD liquid medium at 28°C for 48 hours at a constant temperature, and the concentration of ...

Embodiment 3

[0086] Determination of the inhibitory effect of Pichia anomala (Pichia anomala) YPL6 on diseases according to the present invention

[0087] Effects of YPL6 on postharvest disease control of apples, peaches and pears under storage conditions.

[0088] 1. After the apples are picked, they are soaked in YPL6 bacterial solution for 30-60 seconds, then air-dried at room temperature, and then stored in the dark at a temperature of 1-2°C and a relative humidity of 90-95%. After storage for 10 months, compared with the control group, the control effect was calculated. When the cell concentration is ≥10 8 When cfu / ml, the control effect is above 80%; when the cell concentration is 10 7 When cfu / ml, the control effect is 60%; when the cell concentration is 10 6 When cfu / ml, the control effect is 50%. The effective concentration is ≥10 7 cfu / ml.

[0089] 2. After the peaches are picked, they are soaked in YPL6 bacteria solution for 30-60 seconds, then air-dried at room temperature...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an abnormal pichia anomala and an application thereof, and belongs to the technical field of micrograms. The strain of the abnormal pichia anomala disclosed by the invention is YPL6, with the preservation number of CGMCC No.8041. The abnormal pichia anomala YPL6 and a metabolite product of the pichia anomala YPL6 have the inhibition function on peach postharvest monilinia fructicola, apple postharvest botrytis cinerea, apple postharvest penicillium italicum and pear postharvest penicillium italicum, and have the inhibition function on peach postharvest brown rot, apple postharvest botrytis, pear postharvest penicilliosis and apple postharvest penicilliosis.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a Pichia anomaly and its application. Background technique [0002] Plant fungal diseases have brought huge losses to agricultural production, and fruit postharvest fungal diseases are an important type of disease, resulting in a large reduction in fruit production, seriously affecting the edible value and market value of fruits, and resulting in the economic income of fruit farmers. reduce. In developed countries, the losses caused by postharvest diseases account for more than 15% of the total amount of fruits. In developing countries, due to the lack of necessary prevention and control technology and equipment, the losses caused by postharvest diseases account for 30% or even 30% of the total amount of fruits. higher up. The occurrence of postharvest fruit diseases has seriously affected modern agricultural production, especially the production and processi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16A23B7/155C12R1/84
Inventor 全利红
Owner 北京莱万生物技术有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com