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Screening, identifying and application of antagonistic strain generating volatile bacteriostatic gas

A volatile, antagonistic antimicrobial technology, applied in the prevention and treatment of postharvest diseases of fruits and vegetables, in the field of bacterial liquid fumigation, can solve the problem of reducing the disease prevention effect of chemicals, increasing the chemical residues in the dosage of chemical fungicides, and increasing threats to human health, etc. problem, to achieve the effect of high safety, high-efficiency prevention and control, and simple operation

Active Publication Date: 2016-08-31
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main means of controlling postharvest diseases of fruits and vegetables is to use chemical fungicides. Although good control effects have been achieved, the long-term use of chemical fungicides has caused the bacteria to develop drug resistance, reducing the disease prevention effect of the agents, and eventually leading to chemical fungicides. The dosage of the agent and its chemical residues are greatly increased, and the threat to human health is also increased.

Method used

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  • Screening, identifying and application of antagonistic strain generating volatile bacteriostatic gas
  • Screening, identifying and application of antagonistic strain generating volatile bacteriostatic gas
  • Screening, identifying and application of antagonistic strain generating volatile bacteriostatic gas

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Screening of antagonistic strain 12a

[0025] In the peach orchard without chemical fertilizers and pesticides, the rhizosphere soil of peach trees was collected, and the dilution separation method was used to make 10 -1 、10 -2 、10 -3 and 10 -4 Soil dilution solution, use a pipette gun to draw 10 -2 、10 -3 and 10 -4 Add 0.1mL of the soil dilution solution to the NA medium plate, spread it evenly with a sterile applicator, and mark the serial number. After standing for one hour, reverse the culture plate and cultivate it at 37°C. From the second day of culture, single bacterial colonies were selected according to the characteristics of the colony shape, and transplanted to NA medium respectively, and were used for subsequent experiments after 48 hours of culture. The purified strains were stored on NA slant medium at 4°C.

[0026] Antagonist bacteria were screened by plate buckle method. Inoculate the cake of brown rot fungus in the center of the PDA p...

Embodiment 2

[0028] Embodiment 2: application on the prevention and treatment of peach brown rot

[0029] After inoculating the preserved antagonistic bacteria 12a into 1000mL NB liquid medium for shaking and culturing for 48 hours, invert the bacterial solution (aseptic solution in the lower layer of the control) in the lower layer of the clapboard of the sealed tank, place Feicheng white peaches in the upper layer, and seal the tank lid with vaseline. After fumigation for 20min, the fruit was taken out. In order to make the amount of bacterial infection uniform, a wound of 3 mm × 3 mm × 3 mm was made on each fruit thorn, and then 1 × 10 4 Cfu / mL 20 μL of spore suspension of brown rot fungus, store the above-mentioned treated fruits at 20±1°C, measure the diameter of lesion every 24 hours, and calculate the antibacterial rate, see Table 1 for details. The experiment was repeated three times with 20 fruits per treatment.

[0030] Growth inhibition rate=(diameter of control lesion-diamete...

Embodiment 3

[0031] Embodiment 3: application on the prevention and treatment of apple black spot

[0032] Inoculate the preserved antagonistic bacteria 12a into 1000mL NB liquid medium and shake it for 48 hours, then invert the bacteria solution on the lower layer of the partition of the sealed tank (as compared to the lower layer of sterile solution), place Marshal apples on the upper layer, seal the tank lid with Vaseline, and fumigate for 20 min Then take out the fruit. In order to make the amount of bacterial infection uniform, a wound of 3 mm × 3 mm × 3 mm was made on each fruit thorn, and then 1 × 10 4 20 μL of cfu / mL Alternaria apple spore suspension, store the above-mentioned treated fruits at 20±1°C, measure the diameter of the lesion every 24 hours, and calculate the antibacterial rate, see Table 1 for details. The experiment was repeated three times with 20 fruits per treatment.

[0033] Growth inhibition rate=(diameter of control lesion-diameter of treated lesion) / diameter o...

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Abstract

The invention provides an antagonistic strain 12a generating volatile bacteriostatic gas and application thereof in preventing and treating fruit-vegetable diseases. The antagonistic strain 12a is Bacillus vallismortis and is collected under CGMCC No.12179. The antagonistic strain 12a is obtained by collecting and separating peach tree rhizosphere soil from a Feicheng peach orchard without being applied with chemical fertilizer and pesticide, and the volatile bacteriostatic gas generated in fermentation liquid of the antagonistic strain 12a has strong antagonistic effect on Monilinia fructicola and also has obvious inhibiting effect on Marssonina mali caused by Alternaria mali, sweet potato black spot caused by Ceratocystis fimbriata Ellis.Et Halsted, citrus blue mold caused by Penicillium italicum Wehmer and winter jujube black spot caused by Alternaria alternata. The antagonistic strain 12a can effectively prevent and treat various fruit-vegetable diseases, is a biological preventing and treating potential strain which is good in biological preventing effect, pollution-free, wide in preventing and treating range, safe and ecological and has wide market application prospect.

Description

(1) Technical field [0001] The invention relates to the screening, identification and application of an antagonistic bacteria in the field of microorganisms and biological control, in particular to the application of fumigation of bacterial liquid producing volatile antibacterial gas in the prevention and treatment of post-harvest diseases of fruits and vegetables. (2) Background technology [0002] Huge losses due to postharvest diseases of fruits and vegetables have become a global problem. At present, the main means of controlling postharvest diseases of fruits and vegetables is to use chemical fungicides. Although good control effects have been achieved, the long-term use of chemical fungicides has caused the bacteria to develop drug resistance, reducing the disease prevention effect of the agents, and eventually leading to chemical fungicides. The dosage of the agent and its chemical residue are greatly increased, and the threat to human health is also increased. There...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02A23B7/144A23B7/155C12R1/07
CPCA23B7/144A23B7/155C12N1/02C12N1/205C12R2001/07
Inventor 石晶盈殷晓慧王庆国张畅
Owner SHANDONG AGRICULTURAL UNIVERSITY
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