75 results about "Penicillium charlesii" patented technology

Fungal / bacterial antagonist combinations, a seed coated with one of the combinations and a plant protected from plant pathogens by one of the combinations. The invention is also a fungal / bacterial antagonist combination comprising a Trichoderma virens fungal antagonist and a Bacillus subtilis var. amyloliquefaciens (Bacillus amyloliquefaciens) bacterial antagonist and its use for controlling plant pathogens as a biocontrol agent, bio-pesticide or bio-fungicide. The invention also finds utility as a fungal / bacterial antagonist combination applied to the seed, stalk or leaf that results in an increase in plant yield. Control of early and late season stalk and root rot caused by fungi such as Fusarium, Phythium, Phytophthora and Penicillium in tomatoes, peppers, turf grass, soybeans, sunflower, wheat and corn is achieved.

A process for preparing the xanthonic acid by microbial degradation includes such steps as mixing raw coal with auxiliaries chosen from natural plant carbon source, natural plant protein source, urea and KH2PO4, adding the degradating bacteria chosen from Panicillium sp. LNP6 (CGMCC No.0866) and Bacillus sp.BJB2 (CGMCC No.0867), and fermenting. Its advantages are high activity, small molecule and high oxygen and nitrogen content of product, no environmental pollution, and use of residual dregs are organic fertilizer.

The invention discloses a soil remediation microbial inoculum produced by the fermentation of multiple-thalli mixed liquor and a preparation method thereof. Original strains of azotobacter, P- releasing bacteria, silicate bacteria, biological control bacteria, microzyme, trichoderma, penicillium, lactobacillus and photosynthetic bacteria are selected and mixed with water to form original bacteria solution which is then added into a seeding tank in a proportion of 4 percent to form seed solution. The seed solution is then added into a production tank according to a proportion of 10 percent to form the soil remediation microbial inoculum. The method includes the steps: the azotobacter, the P- releasing bacteria and the silicate bacteria are mixed with water and added into a triangular flask at the temperature controlled at 37 DEG C and cultured for 24 hours in a shaking manner; the biological control bacteria, the microzyme, the trichoderma and the penicillium are mixed with water and cultured for 48 hours to 72 hours in a swing bed and aerobic fermentation is carried out for 16 hours; the lactobacillus is mixed with water and added into a Kolle flask and cultured at the constant temperature of 37 DEG C for 24 hours; and the photosynthetic bacteria are mixed with water, added into a Kolle flask and cultured under a 60W to 80W incandescent lamp for 3 days to 5 days. The microbial inoculum and the preparation method have the advantages of promoting the reproduction of soil microorganisms, carrying out virtuous cycle, improving soil and having simple production technology and low cost.

The invention provides an antagonistic strain 12a generating volatile bacteriostatic gas and application thereof in preventing and treating fruit-vegetable diseases. The antagonistic strain 12a is Bacillus vallismortis and is collected under CGMCC No.12179. The antagonistic strain 12a is obtained by collecting and separating peach tree rhizosphere soil from a Feicheng peach orchard without being applied with chemical fertilizer and pesticide, and the volatile bacteriostatic gas generated in fermentation liquid of the antagonistic strain 12a has strong antagonistic effect on Monilinia fructicola and also has obvious inhibiting effect on Marssonina mali caused by Alternaria mali, sweet potato black spot caused by Ceratocystis fimbriata Ellis.Et Halsted, citrus blue mold caused by Penicillium italicum Wehmer and winter jujube black spot caused by Alternaria alternata. The antagonistic strain 12a can effectively prevent and treat various fruit-vegetable diseases, is a biological preventing and treating potential strain which is good in biological preventing effect, pollution-free, wide in preventing and treating range, safe and ecological and has wide market application prospect.

The invention discloses a paecilomyces hepialid mycelium and a preparation method thereof. The mycelium is obtained by cultivating paecilomyces hepialid via a solid culture medium. The preparation method technically includes: dissecting fresh cordyceps sinensis polypide, separating a paecilomyces hepialid culture in the cordyceps sinensis polypide, cultivating and separating hetero-cultures out, preparing the solid culture medium for sterilizing, inoculating 10% by weight of cultivated and rejuvenated liquid culture on the culture medium for fermentation, and cultivating in an aseptic condition at the temperature of 20-22DEG C for 7-10 days to obtain the mycelium. The mycelium prepared by the preparation method is equal to natural cordyceps sinensis in aspects of nutrition and medical values. The paecilomyces hepialid mycelium is characterized in that under an ordinary 300-600-multiplication optical microscope, the mycelium is 16-40 micrometers in visible length and 3-5 micrometers in root width. The mycelium mainly comprises adenosine which cannot be dissolved by water and can be dissolved by ethyl alcohol.

The invention discloses penicillium oxalicum (fungus) capable of efficiently dissolving phosphorous. The preservation number of the strain is CGMCC No.11126. The strain is separated from soil in a booth, which has been cultivated for many years. The culture medium of penicillium oxalicum is prepared from the following components by weight: 10 grams of glucose, 0.5 gram of ammonium sulfate, 0.3 gram of sodium chloride, 0.3 gram of potassium chloride, 0.03 gram of ferrous sulfate heptahydrate, 0.03 gram of manganese sulfate tetrahydrate, 0.3 gram of magnesium sulfate heptahydrate, 5 grams of tricalcium phosphate, and 20 grams of agar. The culture temperature is 30 DEG C, and the culture pH value is 7.0 to 7.2. The provided penicillium oxalicum has a very strong phosphorus dissolving function, can be used to prepare a soil phosphorus activator for improving the phosphorus utilization rate, and is capable of successfully positioning phosphorus to the plant roots, thus the using amount of phosphorus containing fertilizer is reduced, and the output of fruit and vegetable is increased.

The invention discloses penicillium capable of resisting false smut of rice and an application thereof. The penicillium is prepared through the following steps of: A, ustilaginoidea virens isolation, wherein a ustilaginoidea virens ear is collected, and the ustilaginoidea virens is isolated by adopting a chlamydospore suspension method; B, antibacterial isolation and culture of the ustilaginoideavirens, wherein a soil sample is ground, placed in an aseptic petri dish, and poured into a PDA (potato dextrose agar) culture medium to culture and isolate a single colony of fungus, the isolated fungus strain is inoculated into a PSA (prostate-specific antigen) culture solution for shaking culture, and the fermentation broth is filtered and degermed to obtain an aseptic supernatant sample; and C, antagonist screening of the ustilaginoidea virens, wherein a conidiophore suspension of the ustilaginoidea virens is coated on a PSA panel, an aseptic Oxford plate is placed in the panel, and the aseptic supernatant sample is added into the aseptic Oxford plate to culture penicillium. The invention also provides an application of the antagonist penicillium AF-25 in preparing a biological pesticide for controlling or preventing the false smut of rice. The penicillium has an antagonistic effect on ustilaginoidea virens, and can inhibit spore germination and mycelial growth of the ustilaginoidea virens. The fermentation broth of the penicillium has strong inhibition activity to the false smut.

The present invention relates to a method for preparing dough, in particular to a method for preparing frozen dough. In one aspect the present invention relates to a method for preparing a dough, which method comprises:a) preparing the dough including the step of adding a Penicillium glucose oxidase and a Talaromyces xylanase to at least one dough ingredient; andb) cooling the dough to a temperature in the range of −40 to −5 degrees Celsius.In another aspect the invention relates to a composition comprising a Penicillium glucose oxidase and a Talaromyces xylanase.In a further aspect the invention relates to a dough comprising a Penicillium glucose oxidase and a Talaromyces xylanase, in a further aspect the dough is a frozen dough. The present invention also relates to a baked product a method for preparing a baked product.Further the invention relates to the use of a Penicillium glucose oxidase and a Talaromyces xylanase for improving properties of a dough which has been stored frozen or a baked product prepared from a dough which has been stored frozen.

The invention provides a method for biotransformation of corn stigma with penicillium to obtain flavone. The method utilizes flavones-producing penicillium to ferment a culture solution containing corn stigma, in the fermentation process, the flavone precusor substance in corn stigma is fully utilized for biotransformation to acquire a large amount of accumulated flavone substance, and then purification is carried out to obtain high purity flavone. Specifically, the fermentation medium consists of: 30-50g of corn stigma powder, 2-5g of yeast powder, 0.5-1g of dipotassium hydrogen phosphate, 0.5-1g of magnesium sulfate, 0.5-1g of potassium chloride, 0.01-0.1g of ferrous sulfate, 0.05-0.2g of vitamin B2, 0.05-0.1g of vitamin B6, 0.05-0.3g of vitamin C, 20-40g of maltose, and the balance water added to 1L. The method provided by the invention has the advantages of low cost, high yield, high product purity and good activity, and simple operation steps, and is suitable for industrial production.

The invention relates to corn straw pyroligneous acid. The corn straw pyroligneous acid is prepared by the following method: (1) taking corn straw as a raw material, cutting the corn straw into smallblocks 2-3cm in length, burning the straw at 500-700 DEG C by using a charring furnace, and collecting distillate at the temperature range to obtain crude pyroligneous acid; and (2) performing secondary reduced pressure distillation, and collecting distillate at 98-103 DEG C to obtain refined pyroligneous acid. The invention also relates to application of the corn straw pyroligneous acid in bacteriostasis and a fruit preservative containing the corn straw pyroligneous acid. The corn straw pyroligneous acid provided by the invention has varying degrees of inhibiting effects on four types of bacteria including shigella, salmonella, listeria monocytogenes and staphylococcus aureus, and three types of molds including penicillium, trichoderma virens and trichoderma reesei, and the bacteriostatic effects on the molds are better than the bacteriostatic effects on the bacteria. The corn straw pyroligneous acid has certain preservation effects and can be used as a fruit preservative.