Compounding pesticide containing destruxins of Metarrhizium anisopliae and Paecilomyces fumosoroseus and preparation method thereof

A technology of Paecilomyces chromotheca and Metarhizin, which is applied in the field of mixing synergistic pesticides with Paecilomyces rosacea and the preparation field, and can solve the problem that environmental conditions are greatly affected, the effect is unstable, and the Toxicity is not high enough to achieve the effect of easy promotion, overcoming unstable use effect, and good synergistic effect

Inactive Publication Date: 2009-05-27
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] An object of the present invention is to overcome the shortcomings of the existing Paecilomyces fumigatus and metarhizalin single-dose effects are unstable, are greatly affected by environmental conditions, the toxicity is not high enough, and the effect is slow, and the pests have no effect on the fumigant. The present situation of Penicillium and Metarhizine drug resistance and the need to vigorously develop biological control technology, providing a mixed insecticide of Metarhizin and Paecilomyces fumoscens

Method used

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  • Compounding pesticide containing destruxins of Metarrhizium anisopliae and Paecilomyces fumosoroseus and preparation method thereof
  • Compounding pesticide containing destruxins of Metarrhizium anisopliae and Paecilomyces fumosoroseus and preparation method thereof

Examples

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Embodiment 1

[0022] Example 1 Indoor Toxicity Test of Metarhizine, Paecilomyces fumigatus (CS) Single Agent and Mixed Agent of the present invention on Bemisia tabaci

[0023] In this example, Metarhizin A (DA), Paecilomyces fumigatus (CS) and a mixture prepared according to the following method were used as experimental agents.

[0024] (1) Get conidia of Paecilomyces fumigatus, adopt 0.01~0.05% Tween 80 solution to prepare live conidia concentration as 10 9～11 Individual / L spore suspension, spare;

[0025] (2) Get metarhizine, and use acetone or ethyl acetate to be mixed with the stock solution that active ingredient concentration is greater than 100000mg / L, standby;

[0026] (3) The conidia suspension of the above-mentioned step (1) is mixed with the metarhizin stock solution of the step (2), so that the final concentration remains: the concentration of live conidia is 10 9～11 Individual / L individual / L, metarhizine 10-100mg / L.

[0027] In the indoor toxicity test, Hibiscus rosa-sinen...

Embodiment 2

[0031] Embodiment 2 The field efficacy test of the mixture of the present invention on Bemisia tabaci

[0032] The preparation method of mixture is as embodiment 1.

[0033] The test was carried out in October 2007 on cucumbers grown in greenhouses, with a temperature of 22-30°C, a relative humidity of 81-97%, and an area of ​​30m2. 2 , randomized block design, repeated 4 times, sprayed at the peak of the 2nd instar nymphs of Bemisia tabaci, surveyed once every 2 days after spraying, fixedly inspected 10 points in each plot (each point was about 10cm 2 Leaf area), no less than 100 nymphs at each point, record the total number of insects and (disease) dead insects, calculate the corrected mortality rate, and perform analysis of variance and new multiple range test. The test results are shown in Table 2. The control effect of the synergistic Paecilomyces fumigatus preparation is significantly better than that of pure sporopollen and metarhizine.

[0034] Table 2 Comparison of ...

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Abstract

The invention discloses a pesticide prepared by mixing destruxin and paecilomyces fumosoroseus fungus, and a preparation method thereof. The concentration of the live conidium of the paecilomyces fumosoroseus fungus in the pesticide is 10<9> to 10<11> conidia per liter, and the concentration of the destruxin is 10 mg/L to 100 mg/L; and the preparation method comprises the following steps: preparing the live conidial suspension for the paecilomyces fumosoroseus fungus, preparing the destruxin stock solution, mixing the suspension and the stock solution, and the like. The invention determines the mix proportion of the destruxin and the paecilomyces fumosoroseus fungus, obtains the better synergic effect, overcomes the defects of instable use effect and low toxicity of the single reagent of the destruxin and the paecilomyces fumosoroseus fungus, and also provides a new technical proposal for biological control. The preparation method for the pesticide is simple and feasible, and is easy to be popularized.

Description

technical field [0001] The invention belongs to the technical field of biological pesticides, and in particular relates to a synergistic insecticide mixed with metarhizine and Paecilomyces fumigatus and a preparation method. Background technique [0002] Paecilomyces fumosoroseu is an important entomopathogenic fungus, which has the ability to infect insects such as Homoptera, Lepidoptera, Diptera, Coleoptera, and Hymenoptera, and is mainly used to control whitefly , aphids and other important agricultural and forestry pests. Paecilomyces fumigatus, like other fungal insecticides, has the advantages of not polluting the environment, not producing drug resistance, and continuous effectiveness. Unstable, highly affected by environmental conditions, and slow to take effect. Therefore, the development of a mixture of fungi and chemicals is a good way to overcome its inherent shortcomings. [0003] Metarhizium anisopliae var. anisopliae, an entomopathogenic fungus, is secreted...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01P7/00
Inventor 胡琼波任顺祥
Owner SOUTH CHINA AGRI UNIV
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