Mix fermentation production of soil renovation bacterium agent with multiple bacterials and production method thereof

Abstract

The invention discloses a soil remediation microbial inoculum produced by the fermentation of multiple-thalli mixed liquor and a preparation method thereof. Original strains of azotobacter, P- releasing bacteria, silicate bacteria, biological control bacteria, microzyme, trichoderma, penicillium, lactobacillus and photosynthetic bacteria are selected and mixed with water to form original bacteria solution which is then added into a seeding tank in a proportion of 4 percent to form seed solution. The seed solution is then added into a production tank according to a proportion of 10 percent to form the soil remediation microbial inoculum. The method includes the steps: the azotobacter, the P- releasing bacteria and the silicate bacteria are mixed with water and added into a triangular flask at the temperature controlled at 37 DEG C and cultured for 24 hours in a shaking manner; the biological control bacteria, the microzyme, the trichoderma and the penicillium are mixed with water and cultured for 48 hours to 72 hours in a swing bed and aerobic fermentation is carried out for 16 hours; the lactobacillus is mixed with water and added into a Kolle flask and cultured at the constant temperature of 37 DEG C for 24 hours; and the photosynthetic bacteria are mixed with water, added into a Kolle flask and cultured under a 60W to 80W incandescent lamp for 3 days to 5 days. The microbial inoculum and the preparation method have the advantages of promoting the reproduction of soil microorganisms, carrying out virtuous cycle, improving soil and having simple production technology and low cost.

Description

technical field [0001] The invention relates to microbial liquid submerged fermentation technology, in particular to the production of soil restoration type microbial agent by mixed fermentation of multiple strains and a preparation method. Background technique [0002] my country is a large agricultural country. Long-term petrochemical agriculture has caused soil compaction, pesticides, and chemical fertilizer pollution. In particular, the biological fertility of the soil has dropped significantly. The best way to restore soil fertility is to add organic matter to the soil, especially the addition of soil Beneficial bacteria, especially functional flora, such as nitrogen-fixing, phosphorus-solubilizing, and potassium-solubilizing bacteria. Liquid bacterial agent has the characteristics of high bacterial activity and easy application. It is one of the most effective modifiers for improving soil and improving soil biological fertility. However, most of the liquid bacterial ag...

AI Technical Summary

Problems solved by technology

However, in the past, most of the liquid bacterial agents were compounded by single bacteria, and the proportion of the bacteria after compounding was not easy to control.