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Penicillium effective in prevention and treatment of cucumber fusarium wilt

A technology for cucumber fusarium wilt and Penicillium, applied in the field of microbiology, can solve problems such as pesticide residues, drug resistance, unreasonable chemical agents, etc., and achieve the effects of promoting absorption, promoting growth, and good control effects.

Active Publication Date: 2017-04-26
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Because the long-term unreasonable use of chemical agents has caused many environmental problems
At the same time, it will also cause problems such as drug resistance and pesticide residues.
Although breeding disease-resistant varieties is the best way, it is difficult to select effective and stable resistance lines due to the difficulty in breeding disease-resistant varieties

Method used

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  • Penicillium effective in prevention and treatment of cucumber fusarium wilt
  • Penicillium effective in prevention and treatment of cucumber fusarium wilt
  • Penicillium effective in prevention and treatment of cucumber fusarium wilt

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Isolation of biocontrol bacteria

[0026] Collect 10g of fermented distiller's grains matrix from Pailou teaching base of Nanjing Agricultural University, add it to a 500ml Erlenmeyer flask filled with 90mL of sterile water, place the Erlenmeyer flask on a shaker, and vibrate at 180r / min for 20min to make a matrix suspension. The prepared suspension was allowed to stand for 10 min, and then prepared into 10-fold serial dilutions in sequence. -2 、10 -3 、10 -4 and 10 -5 For serial dilutions, the pipette gun draws 1ml of different series of dilutions and applies it to the solution containing 100mg·l -1 On the PDA medium of streptomycin sulfate, seal the PDA medium plate with a parafilm, and place it in an incubator at 28° C. for about 7 days. After the fungus grows on the plate, pick the fungus that has obvious inhibitory effect on the surrounding fungi as the target fungus, and carry out the next step of re-screening.

[0027] One end on the solid PDA medium plate wi...

Embodiment 2

[0037] Growth-promoting effect of Penicillium sp strain NSY15 on cucumber

[0038] The bacterial plaque of Penicillium sp strain NSY15 made with a hole punch with a diameter of 5mm was transferred to the siderophore phosphate-dissolving medium, cultured at 28°C for 3-5 days, and the color change and hydrolysis circle of the plate were observed. with or without. The appearance of a hydrolysis circle proves that the bacteria has the ability to dissolve inorganic phosphorus. Such as Figure 4 As shown, Penicillium strain NSY15 has the ability to dissolve inorganic phosphorus. It shows that NSY15 can promote the transformation of inorganic phosphorus in the substrate, increase the level of available phosphorus in the soil, and promote the nutrient absorption of plants, thereby promoting the growth of plants.

[0039] Siderophore phosphate solution medium: glucose 10.0g, Ca 3 (PO 4 )2 5.0g, (NH 4 ) 2 SO 4 0.5g, NaCl 0.2g, KCl 0.2g, MgSO 4 ·7H 2 O 0.03g, FeSO 4 0.003g, Mn...

Embodiment 3

[0047] Control Effect of Penicillium sp Strain NSY15 on Cucumber Fusarium Wilt

[0048] Cucumber Fusarium wilt pathogen bacteria liquid: Scrape off the mycelium of the Fusarium oxysporum cucumber-specific pathogen (FOC) activated on the PDA medium and culture it on the liquid PDA medium (180r / min, 28°C) 7 day, filtered through sterilized gauze to obtain the cucumber wilt pathogenic bacteria liquid.

[0049] Cucumber seeds are surface sterilized with 10% sodium hypochlorite solution for 5-10 minutes, then treated in warm water at 55°C for 15 minutes, then soaked in warm water at 30°C for 8 hours, and then placed in a constant temperature box at 28°C for germination, with a humidity of 80%, and kept in the dark , until germination. After the seeds germinate, select plump, uniformly germinated seeds and sow them in a 50-hole tray equipped with a mixed substrate (peat: vermiculite volume ratio 2:1), one seed per hole. After the sowing is completed, the plug tray is placed in a w...

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Abstract

The invention belongs to the field of microbiology and discloses penicillium NSY15 classified and named as Penicillium sp. The penicillium NSY15 is preserved in the China General Microbiological Culture Collection Center on November 5, 2016, and a preservation number is CGMCC No.13188. The penicillium NSY15 is separated from vinasse matrix and capable of effectively preventing and treating cucumber fusarium wilt to reach 52.39% in prevention and treatment effect. In addition, the penicillium NSY15 is capable of dissolving inorganic phosphorus in the matrix to raise available phosphorus level of soil so as to promote cucumber growth, so that the penicillium NSY15 is suitable for being developed and utilized as an agricultural bacterial agent.

Description

technical field [0001] The invention belongs to the field of microbiology and relates to the isolation and application of a penicillium strain. In particular, it relates to a Penicillium fungus which can effectively inhibit the growth of cucumber wilt fungus. Background technique [0002] Cucumber Fusarium wilt is a worldwide devastating soil-borne fungal disease, which occurs all over my country. The pathogen of cucumber wilt is the cucumber-specific type of Fusarium oxysporum. The pathogen invades the plant from the root wound and the root hair growth point, and then destroys the vascular bundles of the root, stem and leaf, causing the cucumber plant to lose the function of absorbing and transporting water, eventually causing Cucumber plants wilt and die. Fusarium wilt can generally cause a 10% to 30% decline in cucumber yield, and it can reach 80% to 90% in severe cases. It is one of the most serious diseases of cucumber and causes the largest economic loss. [0003] C...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P21/00A01P3/00C12R1/80
CPCA01N63/30C12N1/14
Inventor 郭世荣李舒展杜南山施露孙锦束胜
Owner NANJING AGRICULTURAL UNIVERSITY
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