Penicillium ester-III compound, and preparation method, preparation and application thereof

A technology of penicillium and compound, applied in the field of penicillin-Ⅲ compound and its preparation, achieving good effect, safe and reliable effect

Active Publication Date: 2019-09-13
云南省农业科学院生物技术与种质资源研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the application of penicillin-III in the endophytic fungus Pachyphylla as a preparation for the prevention and control of tobacco mosaic disease in the preparation of anti-tobacco mosaic virus drugs

Method used

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  • Penicillium ester-III compound, and preparation method, preparation and application thereof
  • Penicillium ester-III compound, and preparation method, preparation and application thereof
  • Penicillium ester-III compound, and preparation method, preparation and application thereof

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preparation example Construction

[0026] The preparation method of the penicillin-Ⅲ compound of the present invention, the described penicillin-Ⅲ compound is from Chonglou ( Paris L.) Endophytic fungi ( Penicillium sp.), which specifically includes the following steps:

[0027] The penicillin-Ⅲ compound is obtained from Chonglou ( Paris L.) Endophytic fungi ( Penicillium sp.), which specifically includes the following steps:

[0028] (1) Isolation and purification of endophytic fungi

[0029] Clean the fresh tubers of Zhonglou Dianchi, cut them into small pieces, soak them in 70% ethanol for 40s, cut off the outer skin after washing, and insert them into Cha's medium for constant temperature cultivation for 3-7 days until the surface of the Zhonglou tissue pieces and There are fungal colonies growing on the edge, pick a small amount of mycelia on the upper layer of the colony, purify it on the Chase culture medium, and finally transfer it to a PDA slant to obtain the purified strain;

[0030] (2) ...

Embodiment 1

[0050] Clean the soil on the surface of the fresh Prunus dianthus tubers collected in the field with tap water, then cut the roots, stems, leaves, flowers and seeds into small pieces of suitable size, then soak them in 70% ethanol for 40 seconds, remove the ethanol and rinse with sterile water 3 times, rinse with 0.1% HgCl2 solution for 2.5min, and rinse with sterile water 5 times. Blot the surface of the material with a piece of sterile filter paper, cut off the outer skin, insert Chapeauer medium (6 pieces per plate) and place in a constant temperature incubator at 28°C for cultivation. After 3 to 7 days, there were fungal colonies growing on the surface and edge of the Parisiana tissue block, and a small amount of mycelium on the upper layer of the colony was picked for multi-stage purification on Chase culture medium, and finally transferred to the PDA slant to obtain the purified strain.

[0051] Temporary slides were made of the cultured fungi, stained with potassium per...

Embodiment 2

[0058] Preparation of 0.5 μg / mL penicillin-III aqueous solution:

[0059] Weigh 1 μg of penicillin-III compound and dissolve it in a small amount of DMSO solution, stir and mix thoroughly, after it is fully dissolved, add the remaining amount of distilled water to a final volume of 2 mL to make an aqueous solution, that is, 0.5 μg / mL of penicillin Mycosyl-III aqueous solution.

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Abstract

The invention discloses a penicillium ester-III compound, and a preparation method, a preparation and application thereof. The penicillium ester-III compound is isolated from the endophytic fungus (Penicillium sp.) of Paris L., and has a molecular formula of C21H26O7. The structural formula of the compound is as described in the specification. The compound is named as penicillium ester-III. The preparation disclosed in the invention is a water aqua, a tablet, a granule or a microemulsion prepared by adding pharmaceutically acceptable adjuvants and / or an excipient to the penicillium ester-III compound. The application refers to the application of penicillium ester-III in the preparation of drugs for resisting tobacco mosaic viruses. Penicillium ester-III can not only significantly inhibit the initial infection of TMV on a host, but also has certain inactivation effect on TMV, thereby reducing the chance that viruses enter host cells and further replicates and propagates, so protection effect is performed on the host. The pharmaceutical preparation of the invention is non-toxic and harmless to human beings and animals, and safe and reliable to use, has good effect on preventing and treating tobacco mosaic virus diseases, and can be used for preparing pharmaceutical preparations applied to prevention and treatment of tobacco mosaic virus diseases.

Description

technical field [0001] The invention belongs to the technical field of medicines, and in particular relates to a penicillin-III compound and its preparation method, preparation and application. Background technique [0002] Plant virus disease is a kind of plant disease second only to fungi and bacteria. It is very harmful and difficult to control. It is called "plant cancer". tobacco mosaic virus ( Tobacco mosaic virus , TMV) is a pathogen of tobacco mosaic disease, etc., which can infect more than 300 kinds of plants in 30 families, and seriously harms tobacco, tomato and other food and economic crops, causing huge economic losses to agricultural production. In the current agricultural production, chemical pesticides are the most widely used to control TMV, but the effect of chemical control is limited, and it is easy to cause excessive pesticide residues, which cannot meet the requirements of sustainable agricultural development. Therefore, there is an urgent need to de...

Claims

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Application Information

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IPC IPC(8): C07C69/732A01N37/38A01P1/00C12P7/62C12R1/80
CPCA01N37/38C07C69/732C12P7/62
Inventor 陈永对李干鹏董家红张俊郑雪吴阔苏晓霞张洁张仲凯
Owner 云南省农业科学院生物技术与种质资源研究所
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