A kind of iridoid compound and its preparation method, preparation and application

A technology of iridoids and compounds, applied in the field of iridoids and their preparation, to achieve safe, reliable and good effects

Active Publication Date: 2018-06-19
YUNNAN MINZU UNIV +1
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, although some active substances have no effect on these three parts, they can activate the host to produce systemic resistance

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of iridoid compound and its preparation method, preparation and application
  • A kind of iridoid compound and its preparation method, preparation and application
  • A kind of iridoid compound and its preparation method, preparation and application

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0025] The preparation method of the iridoid compound according to the present invention is prepared by taking Southwestern Swertia as a raw material through the steps of raw material treatment, extract extraction, organic solvent extraction, elution and separation, specifically comprising:

[0026] A. Raw material processing: Take the dried Southwest Swertia japonica and crush it through a 50-100 mesh sieve for later use;

[0027] B. Extraction of extract: add methanol with a volume percentage concentration of 75-85% of the volume percentage of 2-5 times the solid-liquid volume ratio to the pre-treated Southwest Swertia sonic extraction 5-10 times, each time for 3-5h, and combine The extract is filtered, and the extract is concentrated under reduced pressure to a relative density of 1.2 to 1.3 to obtain an extract;

[0028] C. Organic solvent extraction: add water with a solid-to-liquid volume ratio of 1 to 3 times to the extract and stir for 5 to 30 minutes until it becomes ...

Embodiment 1

[0043]Take the shade-dried Southwest Swertia japonica and crush it, coarsely crush it through a 50-mesh sieve; extract 5 times with 80% methanol, 2 hours each time; combine the extracts, filter to remove residues, combine the filtrates, and then concentrate under reduced pressure to obtain leaching extracts with a relative density of 1.2. Add 1 volume of water to the extract and stir until it becomes suspended, extract twice with 98% dichloromethane solution, 15 min each time; combine the extracts, decolorize with MCI, and elute with 75% methanol , collect the eluate; then desugar on the D900 macroporous resin column, using methanol / water with a volume ratio of 0:1, 9:1, 8:2, 6:4, 3:7 and 1:0 (V / V) Solution elution, collect the eluate, monitor by TLC, combine the same parts, concentrate to get the extract, 0:1 part is separated by HPLC, eluted with 70% methanol aqueous solution, and finally Agel semi-preparative column Carry out purification, elute with 70% methanol aqueous s...

Embodiment 2

[0046] Take the shade-dried Southwest Swertia japonica and crush it, coarsely crush it through a 50-mesh sieve; extract it with 75% methanol for 10 times, each time for 3 h; combine the extracts, filter and remove the slag, combine the filtrates, and then concentrate under reduced pressure to obtain leaching powder with a relative density of 1.3. Add 3 times the volume of water to the extract and stir until it becomes a suspension, extract it with 95% dichloromethane solution for 5 times, each time for 30 min; combine the extracts, decolorize with MCI, and elute with 75% methanol , collect the eluate; then desugar on the D900 macroporous resin column, using methanol / water with a volume ratio of 0:1, 9:1, 8:2, 6:4, 3:7 and 1:0 (V / V) Solution elution, collect the eluate, monitor by TLC, combine the same parts, concentrate to get the extract, 0:1 part is separated by HPLC, eluted with 70% methanol aqueous solution, and finally Agel semi-preparative column Carry out purification,...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses an iridoid compound and its preparation method, preparation and application. The iridoid compound is isolated from Southwest Swertia, its molecular formula is C16H22O11, and its structural formula is: the compound Named: Eustomoside. The preparation method uses Southwest Swertia as a raw material, and is prepared through the steps of raw material treatment, extract extraction, organic solvent extraction, elution and separation. The preparation is prepared by adding pharmaceutical adjuvants and / or excipients to the iridoid compound to prepare water, tablet, granule or microemulsion. The application is the application of the iridoid compound in the preparation of medicines for preventing and treating TMV. The pharmaceutical preparation of the invention is not only non-toxic and harmless to humans and animals, safe and reliable to use, but also has good effect on preventing and treating TMV, and can be used for preparing the pharmaceutical preparation for preventing and treating TMV.

Description

technical field [0001] The invention belongs to the technical field of phytochemistry, and in particular relates to an iridoid compound and a preparation method, preparation and application thereof. Background technique [0002] tobacco mosaic virus ( Tobacco mosaic virus , TMV) is the pathogen of tobacco mosaic disease, etc. TMV has a wide range of hosts and can infect more than 300 kinds of plants in 30 families. It is seriously harmful to food crops such as tobacco and tomato, and causes huge economic losses to agricultural production. It is listed as the most important ten species of molecular plant pathology. The first plant virus. In the current agricultural production, chemical pesticides are the most widely used to control TMV, but the effect of chemical control is limited, and it is easy to cause excessive pesticide residues, which cannot meet the requirements of sustainable agricultural development. Therefore, there is an urgent need to develop new antiviral age...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/04C07H1/08A01N43/90A01N25/02A01N25/04A01N25/12A01N25/34A01P1/00
Inventor 李干鹏陈永对张仲凯董家红左马怡曾亮张洁吴阔
Owner YUNNAN MINZU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap