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A kind of penicillin-III compound and its preparation method, preparation and application

A penicillium and compound technology, applied in the field of penicillin ester-III compound and its preparation, to achieve good effect, safe and reliable effect

Active Publication Date: 2021-11-12
云南省农业科学院生物技术与种质资源研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the application of penicillin-III in the endophytic fungus Pachyphylla as a preparation for the prevention and control of tobacco mosaic disease in the preparation of anti-tobacco mosaic virus drugs

Method used

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  • A kind of penicillin-III compound and its preparation method, preparation and application
  • A kind of penicillin-III compound and its preparation method, preparation and application
  • A kind of penicillin-III compound and its preparation method, preparation and application

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preparation example Construction

[0026] The preparation method of the penicillin ester-III compound of the present invention, the penicillin ester-III compound is obtained from Chonglou ( Paris L.) Endophyte ( Penicillium sp.), which specifically includes the following steps:

[0027] The penicillin ester-III compound was obtained from Chonglou ( Paris L.) Endophyte ( Penicillium sp.), which specifically includes the following steps:

[0028] (1) Separation and purification of endophytic fungi

[0029] The fresh tubers of Dianzhonglou were washed and cut into small pieces, then soaked in 70% ethanol for 40s, rinsed and cut off the outer epidermis, and inserted into the Chase medium for constant temperature cultivation for 3 to 7 days to the surface of the Dianzhonglou tissue block and the surface. There is a fungal colony growing on the edge, pick a small amount of mycelium on the upper layer of the colony and purify it on Cha's medium, and finally transfer it to the PDA slant to obtain a purified...

Embodiment 1

[0050] Fresh Dianzhonglou tubers collected in the field were washed with tap water to clean the surface soil, then cut the roots, stems, leaves, flowers and seeds into small pieces of suitable size, then soaked in 70% ethanol for 40s, and rinsed with sterile water after removing the ethanol 3 times, rinsed with 0.1% HgCl2 solution for 2.5 min, and rinsed 5 times with sterile water. The surface of the material was blotted dry with sterile filter paper, and the outer epidermis was cut off, and then inserted into Charlie's medium (6 pieces per plate) and placed in a 28°C constant temperature incubator for culture. After 3 to 7 days, fungal colonies grew on the surface and edge of the Dianzhonglou tissue block. A small amount of mycelium on the upper layer of the colony was picked for multi-stage purification on Cha's medium, and finally transferred to the PDA slant to obtain purified strains.

[0051] Temporary slides were made of the cultured fungi, stained with potassium perman...

Embodiment 2

[0058] Preparation of 0.5 μg / mL penicillin ester-III aqueous solution:

[0059] Weigh 1 μg of penicillin ester-III compound and dissolve it in a small amount of DMSO solution, stir and mix thoroughly, and after it is fully dissolved, add the remaining amount of distilled water to make a final volume of 2 mL to prepare a water preparation, that is, to obtain 0.5 μg / mL of cyanobacteria. Mycosteryl-III aqueous solution.

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Abstract

The invention discloses a penicillin-III compound and a preparation method, preparation and application thereof. The penicillin-Ⅲ compound is from the building ( Paris L.) Endophytic fungi ( Penicillium sp. ), and its molecular formula is C 21 h 26 o 7 , the structural formula is: The compound is named: penicillin-Ⅲ. The preparation disclosed in the present invention is prepared by adding pharmaceutical adjuvants and / or excipients to the penicillin-III compound to prepare water, tablet, granule or microemulsion. The application is the application of penicillin-III in the preparation of anti-tobacco mosaic virus medicine. Penicillin-III can not only significantly inhibit the initial infection of TMV to the host, but also has a certain passivation effect on TMV, thereby reducing the chance of the virus entering the host cell and further replicating and multiplying, and playing a role in protecting the host. The pharmaceutical preparation of the invention is not only non-toxic and harmless to humans and animals, safe and reliable to use, but also has good effect on preventing and treating tobacco mosaic virus disease, and can be used for preparing the pharmaceutical preparation for preventing and treating tobacco mosaic virus disease.

Description

technical field [0001] The invention belongs to the technical field of medicine, and particularly relates to a penicillyl ester-III compound and its preparation method, preparation and application. Background technique [0002] Plant virus diseases are a class of plant diseases second only to fungi and bacteria, which are harmful and difficult to control, and are known as "plant cancers". Tobacco mosaic virus ( Tobacco mosaic virus , TMV) is a pathogen such as tobacco mosaic disease, which can infect more than 300 kinds of plants in 30 families, causing serious harm to crops such as tobacco and tomato, and causing huge economic losses to agricultural production. In the current agricultural production, chemical pesticides are the most widely used to control TMV, but the chemical control effect is limited, and it is easy to cause excessive pesticide residues, which cannot meet the requirements of sustainable agricultural development. Therefore, there is an urgent need to dev...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C69/732A01N37/38A01P1/00C12P7/62C12R1/80
CPCA01N37/38C07C69/732C12P7/62
Inventor 陈永对李干鹏董家红张俊郑雪吴阔苏晓霞张洁张仲凯
Owner 云南省农业科学院生物技术与种质资源研究所
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