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Method for screening strains of penicillium sp. and application thereof in degradation of glyphosate waste water

A technology for glyphosate wastewater and screening methods, which is applied in the field of application of the strain in degrading glyphosate wastewater, and can solve problems such as incomplete mineralization of organic matter and being in the research stage

Active Publication Date: 2010-05-19
CHINA THREE GORGES UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although TiO 2 The advanced oxidation technology represented by Fenton and Fenton has the advantages of loose conditions and relatively simple water pollution treatment, but Fenton and TiO 2 Oxidation methods and technologies are incomplete for the mineralization of organic matter, and the technology is still in the research stage and needs further development

Method used

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  • Method for screening strains of penicillium sp. and application thereof in degradation of glyphosate waste water
  • Method for screening strains of penicillium sp. and application thereof in degradation of glyphosate waste water
  • Method for screening strains of penicillium sp. and application thereof in degradation of glyphosate waste water

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Experimental program
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Effect test

preparation example Construction

[0030] The preparation method of Penicillium CTGU-CGL-002:

[0031] (1), the isolation steps of this strain

[0032] enrichment of a strain

[0033] In a 250mL Erlenmeyer flask, add 30mL enrichment medium, 10mL glyphosate wastewater, and then add 10mL sludge (total 50mL) that has been aerated for 24 hours, place in a shaker, and culture at a constant temperature of 30°C for 3 days.

[0034] The domestication process of the b strain

[0035] Add glyphosate wastewater, bacterial solution and inorganic salt medium under sterile conditions, the volume of glyphosate wastewater gradually increases from 10mL, 15mL to 30mL, the total volume of the culture solution is 50mL, and placed in a shaker at a constant temperature of 30°C Shaking culture, 4d is an acclimatization cycle, and three cycles of acclimatization are carried out. After each acclimatization period, the COD value of each bottle of culture solution was measured, and the degradation rate was calculated. In each group, ...

Embodiment 1

[0051] In a 250mL Erlenmeyer flask under sterile conditions, add 5mL glyphosate wastewater, 10mL bacterial solution, and inorganic salt medium (total 50mL), place in a shaker, and culture at a constant temperature of 30°C for 4 days. After 4 days, the strain's degradation rate of glyphosate wastewater reached 28.15%.

Embodiment 2

[0053] In a 250mL Erlenmeyer flask under sterile conditions, add 10mL of glyphosate waste water, 10mL of bacterial liquid, and inorganic salt medium (50mL in total), place in a shaker, and culture at a constant temperature of 30°C for 4 days. After 4 days, the strain's degradation rate of glyphosate wastewater reached 56.08%.

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Abstract

The invention relates to a method for screening strains of penicillium sp. and application thereof in degradation of glyphosate waste water. In the method, the glyphosate waste water is used as an only carbon source to separate engineering bacteria capable of effectively degrading the glyphosate waste water from active sludge in nature by selective enrichment, acclimation, streak isolation and purification. Morphologic and cultural characteristics and the observation of physiological and biochemical experiment results show that the penicillium sp. has high degradation effect on the glyphosate waste water. The engineering bacteria of the invention can be applied to the treatment for the glyphosate waste water and purification treatment for an organic pollutant in municipal waster water. The optimum conditions for the growth of penicillium sp. comprise: the temperature of 30 DEG C, a pH value of 7.0, and substrate concentration of 600ml*l-1. Under the optimum growth conditions, the engineering bacteria has a maximum degradation rate of the glyphosate waste water of 95.52 percent, so the engineering bacteria has higher mineralization degree, low cost of processing equipment, simple operation and wide application.

Description

technical field [0001] The invention relates to a penicillium strain and a screening method thereof, and also relates to the application of the strain in degrading glyphosate wastewater. Background technique [0002] The chemical name of glyphosate is N-phosphine carboxymethylglycine, which belongs to the group of aminomethylene phosphines containing carboxylic acid groups. Glyphosate wastewater has the characteristics of high concentration and serious pollution. The pollutants contained in wastewater mainly include methanol, formaldehyde, glyphosate and salts. Nowadays, the treatment of glyphosate wastewater in my country is also an urgent problem to be solved. If it is not treated and discharged into the environment, it will inevitably pollute water sources, spread diseases, and endanger people's physical and mental health. [0003] According to the process and operation status of wastewater treatment at home and abroad, the current environmental treatment technology is ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C02F3/34C02F3/02C12R1/80C02F101/38
CPCY02W10/10
Inventor 黄应平王晓星梅朋森
Owner CHINA THREE GORGES UNIV
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