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Production of flavicidic acid by microorgan degradation

A microbial degradation and fulvic acid technology, applied in fermentation and other directions, can solve the problems of low flocculation limit of fulvic acid, polluting the environment with secondary waste, no industrial production value, etc., and achieve sustainable utilization, large flocculation limit, and activity strong effect

Inactive Publication Date: 2004-07-07
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the extraction rate of this method is low and has no industrial production value
Moreover, the fulvic acid produced by the chemical method has a low flocculation limit, low activity, and produces secondary waste that pollutes the environment.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] 1) Ingredients

[0058] 45% brown coal + 14% sawdust + 14% bean cake + 25% straw (corn or rice straw) + 2% urea + 0.1% KH 2 PO 4

[0059] Crush lignite (from Huolin Gol Coal Mine in Inner Mongolia) to about 50 mesh, and crush other auxiliary materials to about 2 mm;

[0060] 2) Fermentation

[0061] Mix and stir various materials evenly, add 2 times the weight of water (urea and KH 2 PO 4Dissolve in water and add), let stand for 30 minutes and stir evenly, add 10% inoculum to Penicillium (solid koji or liquid culture: V / W per gram of wet material or W / W), cultivate at 28℃~30℃ 2 days, starting from the 12th hour, stirring every 5-6 hours. After 2 days, re-insertion of spores (the inoculum amount is the same as above), and let stand and ferment for 6-7 days.

[0062] 3) Extraction step: After fermentation is completed, add tap water according to material (dry): water=1:20, room temperature (or keep 60℃) for two hours, intermittent stirring, settling for two hours, separate t...

Embodiment 2

[0071] 1) Ingredients

[0072] 56% lignite (from Sanmenxia, ​​Henan) + 20% bean cake + 12% rice bran + 10% sawdust + 2% urea + 0.1% KH 2 PO 4

[0073] 2) Operation steps

[0074] Mix and stir all kinds of materials evenly, add appropriate amount of water (based on the principle of wet and loose materials, urea and KH 2 PO 4 Dissolve in water and add), let stand for 30 minutes and stir evenly, add 10% inoculum to Penicillium (solid koji or liquid culture: V / W per gram of wet material or W / W), cultivate at 28℃~30℃ 2 days, starting from the 12th hour, stirring every 5-6 hours. After 2 days, re-insertion of spores (the inoculum amount is the same as above), and the fermentation is allowed to stand for 6-7 days. After fermentation, 18-25% of fulvic acid was extracted at 20°C by the method described in Example 1, and the residue was further extracted with water or directly used as a humic acid fertilizer.

[0075] The method for separating and cultivating strains and the identification...

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PUM

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Abstract

A process for preparing the xanthonic acid by microbial degradation includes such steps as mixing raw coal with auxiliaries chosen from natural plant carbon source, natural plant protein source, urea and KH2PO4, adding the degradating bacteria chosen from Panicillium sp. LNP6 (CGMCC No.0866) and Bacillus sp.BJB2 (CGMCC No.0867), and fermenting. Its advantages are high activity, small molecule and high oxygen and nitrogen content of product, no environmental pollution, and use of residual dregs are organic fertilizer.

Description

Technical field [0001] The invention relates to a method for producing fulvic acid by using microorganisms to degrade low-energy coal. Background technique: [0002] Fulvic acid is a component of humic acid with a small molecular weight (generally considered to be less than 500D) and high activity. It can be widely used in industry, agriculture, medicine, animal husbandry, etc., such as improving plant drought resistance, as a fertilizer, pesticide synergist , Plant growth stimulants, raw materials for the pharmaceutical industry, etc. [0003] At present, fulvic acid is produced by chemical methods, mainly extracted from weathered coal: [0004] (1) Use acetone sulfate method [0005] The weathered coal after water separation is mixed with water-containing acetone, suspended by stirring, and then sulfuric acid is added to stir the reaction. After the natural sedimentation, the solvent is removed from the reaction supernatant to obtain a fulvic acid product. Due to the low conten...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/40
Inventor 袁红莉陈文新
Owner CHINA AGRI UNIV
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