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Method for biotransformation of corn stigma with penicillium to obtain flavone

A technology of biotransformation and penicillium, applied in the field of obtaining flavonoids, can solve the problems of low basic content of flavonoids, the extraction rate needs to be improved, and shorten the extraction time, etc., and achieve the effects of less by-products, easy separation and purification, and environmental friendliness

Inactive Publication Date: 2019-02-01
湖南中茂生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Ultrasonic-assisted extraction has the advantages of relatively high extraction rate, short time, saving solvents, and reducing the impact of high temperature on the extracted components, but the extraction rate still needs to be improved
The ultrasonic-double-enzyme synergistic extraction method shortens the extraction time and greatly improves the extraction rate of flavonoids, but it also has the disadvantage of high cost.
The above methods all have the problems of large consumption of raw materials and low basic content of flavonoids

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The preparation of the fermentation medium used in this embodiment: corn silk powder 50g, yeast powder 3g, dipotassium hydrogen phosphate 1g, magnesium sulfate 0.5g, potassium chloride 0.5g, ferrous sulfate 0.01g, vitamin B2 0.1g, vitamin B6 0.1g, vitamin C 0.1g, maltose 30g, add water to 1L.

[0029] A method for obtaining flavonoids by utilizing Penicillium biotransformation corn silk, comprising the steps of:

[0030] S1: After activating Penicillium CGMCCNO.6113 on the activation medium, the concentration of the bacterial suspension was 10 4 CFU / ml, inoculate 1ml into the fermentation medium and culture it on a shaker for 7 days to obtain a fermentation broth, wherein the shaker speed is 200rpm, the culture temperature is 35°C, and the initial pH is 7;

[0031] S2: Take the fermentation broth in step S1 and add disintegrating enzymes to disrupt the cells and treat them in a water bath at 37°C for 5 minutes to release the intrabacterial products. Centrifuge at 200...

Embodiment 2

[0036] Preparation of fermentation medium in this example: 50 g of corn silk powder, 5 g of yeast powder, 0.5 g of dipotassium hydrogen phosphate, 0.5 g of magnesium sulfate, 0.5 g of potassium chloride, 0.01 g of ferrous sulfate, 0.05 g of vitamin B2, and vitamin B6 0.05g, vitamin C 0.05g, maltose 40g, add water to 1L.

[0037] A method for obtaining flavonoids by utilizing Penicillium biotransformation corn silk, comprising the steps of:

[0038] S1: After activating Penicillium CGMCCNO.6113 on the activation medium, the concentration of the bacterial suspension was 10 6 CFU / ml, inoculate 1ml into the fermentation medium and culture it on a shaker for 5 days to obtain a fermentation broth, wherein the shaker speed is 150rpm, the culture temperature is 30°C, and the initial pH is 6.6;

[0039] S2: Take the fermentation broth in step S1 and add 2000u of disintegrating enzyme to break the cells and treat them in a water bath at 37°C for 10 minutes to release the intrabacteria...

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Abstract

The invention provides a method for biotransformation of corn stigma with penicillium to obtain flavone. The method utilizes flavones-producing penicillium to ferment a culture solution containing corn stigma, in the fermentation process, the flavone precusor substance in corn stigma is fully utilized for biotransformation to acquire a large amount of accumulated flavone substance, and then purification is carried out to obtain high purity flavone. Specifically, the fermentation medium consists of: 30-50g of corn stigma powder, 2-5g of yeast powder, 0.5-1g of dipotassium hydrogen phosphate, 0.5-1g of magnesium sulfate, 0.5-1g of potassium chloride, 0.01-0.1g of ferrous sulfate, 0.05-0.2g of vitamin B2, 0.05-0.1g of vitamin B6, 0.05-0.3g of vitamin C, 20-40g of maltose, and the balance water added to 1L. The method provided by the invention has the advantages of low cost, high yield, high product purity and good activity, and simple operation steps, and is suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of biological extraction, and more specifically relates to a method for obtaining flavonoids by using penicillium to biotransform corn silk. Background technique [0002] Corn is one of the important gramineous grain crops in the world today. Corn silk is its dried filaments and stigmas. It is a traditional Chinese medicinal material in my country. Studies have shown that corn silk contains a variety of biologically active components, such as volatile oil, saponins, flavonoids, etc., which have a wide range of pharmacological effects. Among them, there are many types of flavonoids, and its chemical structure is complicated. Its chemical structure contains a ketone hydroxyl , and most of its hydroxyl derivatives are yellow, so they are called flavonoids. Flavonoids have remarkable pharmacological effects, such as reducing myocardial oxygen consumption, increasing coronary artery and cerebrovascular flow, low...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/06C12R1/80
CPCC12P17/06
Inventor 危志刚赵勇彪李佳莲赵勇志易娜
Owner 湖南中茂生物科技有限公司
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