7results about How to "Mild fermentation conditions" patented technology

The invention belongs to the field of biological fermentation feeds, and discloses a preparation method and application of a Sophora alopecuroides feed based on multi-bacterial enzyme synergistic fermentation, and particularly relates to a preparation method of a Sophora alopecuroides feed based on multi-bacterial enzyme synergistic fermentation by taking Sophora alopecuroides straw as a raw material, adopting a complex microbial inoculant composed of lactobacillus plantarum, saccharomyces cerevisiae, bacillus subtilis and aspergillus niger, cooperating with cellulase and beta-mannase, and performing multi-bacterial enzyme synergistic fermentation. The compound is prepared through a two-stage fermentation process of aerobic enzyme production and anaerobic detoxification. The invention provides a preparation method and application of a Sophora alopecuroides feed based on multi-bacterial enzyme synergistic fermentation, toxic alkaloids in Sophora alopecuroides can be efficiently degraded, the crude protein content can be significantly increased, and fibers can be degraded, and after the prepared fermented feed replaces corn in daily ration of mutton sheep, the daily gain of the mutton sheep can be significantly increased, the feed-to-weight ratio can be reduced, and the production cost can be reduced. The flavor and the nutritional value of the mutton are improved, high-valued and safe utilization of toxic agricultural wastes is realized, and a new scheme is provided for green breeding and high-quality animal product production.

This invention discloses a method and application for treating moldy corn to synthesize polyhydroxy fatty acids (PHA) using a compound enzyme preparation, relating to the fields of fermentation engineering and waste resource utilization technology. The method for treating moldy corn to synthesize PHA using a compound enzyme preparation includes the following steps: 1) screening and culturing highly efficient halophilic bacteria as inoculum strains for open-enzyme PHA synthesis; 2) pretreating moldy corn with a compound enzyme preparation composed of α-amylase, β-amylase, and glucoamylase; 3) using the moldy corn hydrolysate as a fermentation carbon source, inoculating with PHA-producing halophilic bacteria for open-enzyme fermentation. This invention constructs a complete process system of specific halophilic bacteria screening – moldy corn enzymatic pretreatment – ​​open-enzyme fermentation to synthesize PHA, realizing the resource utilization and high-value utilization of moldy corn. Compared with existing technologies, it has significant economic, environmental, and industrial value, with outstanding comprehensive beneficial effects.

The invention discloses a single-bacterium fermentation compounding method of cattle feed, and relates to the technical field of feed fermentation. Comprising the following steps: preparing a single-bacterium fermentation bacterial material, the single-bacterium fermentation bacterial material comprises 42.25-47.25 parts of wheat bran, 19-21 parts of soybean meal, 14.25-15.75 parts of corn flour, 19-21 parts of rice hull powder and 0.003-0.005 part of a bacterial liquid, and strains used by the bacterial liquid comprise six probiotics including saccharomyces cerevisiae, bacillus subtilis, bacillus licheniformis, lactobacillus plantarum, enterococcus faecalis and lactobacillus acidophilus; the six probiotics are subjected to independent solid fermentation, the prepared fermentation fungus materials are compounded in proportion to obtain a compound fermentation fungus material, and the compound fermentation fungus material is fed according to cows with different weights. Through the technical route of single-bacterium independent fermentation, the problem of mutual inhibition generated during compound fermentation of various strains is avoided, high-density propagation of the strains in the fermentation process is guaranteed, the compounded feed can meet the nutritional requirements of cattle in different stages, and the yield of the cattle is improved. The feed digestion and absorption rate is effectively improved, the cattle immunity is enhanced, and the growth performance is promoted.

This invention relates to a recombinant bacterial cell for de novo synthesis of xanthocyanin WGX-50 using glucose as a carbon source, its construction method, and its applications. The recombinant bacterial cell uses *Escherichia coli* as the host strain and integrates the following three heterologous biosynthetic modules: a 3,4-dimethoxyphenylethylamine de novo synthesis module, which contains an enzyme system catalyzing the generation of dopamine from L-tyrosine and further stepwise methylation of dopamine to 3,4-dimethoxyphenylethylamine; a cinnamoyl-CoA synthesis module, which contains an enzyme system catalyzing the generation of cinnamoyl-CoA from phenylalanine or glucose metabolic intermediates; and an amidation module, which contains an acyltransferase capable of catalyzing the condensation of 3,4-dimethoxyphenylethylamine and cinnamoyl-CoA to generate xanthocyanin WGX-50. This invention achieves, for the first time, the de novo biosynthesis of xanthocyanin in microorganisms, completely eliminating dependence on the expensive exogenous precursor 3,4-dimethoxyphenylethylamine and significantly reducing raw material costs.

This invention discloses a strain of *Bacillus glabra*, a microbial inoculant, and its applications. *Bacillus glabra* (… Bacillus altitudinis Named Bacillus altitudinis The strain number is M1118, the accession number is GDMCC NO:67333, and the accession date is November 21, 2025. Using the *Bacillus hygroscopicus* strain of this invention to extract flavonoids from bamboo leaves significantly improves extraction efficiency: through microbial fermentation, the extraction rate of bamboo leaf flavonoids is increased by more than 50% compared to traditional methods. It is environmentally friendly: the fermentation conditions are mild, requiring no heating or electrical equipment. It is simple to operate and low in cost: the preparation of the inoculum and the fermentation process are easily scalable, reducing the cost of bamboo leaf processing. It allows for comprehensive resource utilization: the fermentation residue can be converted into organic fertilizer, achieving full resource utilization.

The invention relates to an antibiotic-free biological fermentation compound feed for poultry and a preparation method thereof, and belongs to the technical field of feeds, the feed takes a fermentation basic raw material as a carrier, plant extracts and chemical addition components are added, and the synergistic effects of resisting bacteria, diminishing inflammation, regulating intestinal flora, promoting nutrient absorption and improving the immunity of poultry are achieved in a biological fermentation synergistic manner; and antibiotic residues are avoided, so that the safety of poultry products is guaranteed.

This invention belongs to the field of biomedical technology, specifically disclosing a method for extracting and applying fermented sword bean oil. The method involves first activating Bacillus subtilis, then inoculating it onto sword bean powder for solid-state fermentation. After drying and pulverizing the fermented material, extraction is performed using an extraction solvent on a shaker and followed by filtration. The filtrate is then evaporated under vacuum to remove the solvent, yielding fermented sword bean oil. This method disrupts the cell walls of sword beans, promoting oil release and increasing the oil yield. After fermentation, the content of polyunsaturated fatty acids in the oil increases to 25.763±0.663%, with the addition of pentadecenoic acid and erucic acid, as well as DPPH and ABTS. + The free radical scavenging ability and iron ion reducing ability are significantly enhanced, resulting in improved antioxidant activity. The process conditions of this invention are mild, the process is simple, the energy consumption is low, and it is easy to scale up. The resulting oil can be used in the food, health product, and pharmaceutical industries.