38results about How to "Mild fermentation conditions" patented technology

The invention discloses a bioanalysis preparation technique of a biological organic fertilizer by chicken manure. High-efficient and green chicken mature fermenting biological organic fertilizer is developed through a mixed bacteria aerobiotic two-step fermenting technique, wherein an one-step fermenting flora comprises aspergillus, thermophilic sporotrichum, saccharomycetes, bacillus subtilis, thermophilic heterotrophism ammonia oxidizing bacteria; a two-step aerobiotic fermenting flora comprises EM fungicide, azotobacter chroococcum, and bacillus licheniformis. The technique adopts multi-bacterium mixing fermentation; through combination of metabolic capabilities, the complex metabolism which is hard to complete by single culture is completed; through synergic fermentation between microorganisms, the chicken mature is effectively transformed, the hygienic index reaches the national standard, and the product decomposition and growth promoting index are significantly promoted. The fermenting technique is simple and high-efficient, the deodorization and sterilizing effects are good; besides, the production cost is low, and the technique is easy to carry out industrial scale production.

The invention discloses a fermentation and optimization method for tobacco flavors, which takes tobacco residual materials as raw materials, such as tobacco stems, tobacco dusts and secondary tobacco leaves, the tobacco residual materials are put into a fermentor, sugar and inorganic salt, tobacco flavor bottom materials, and water are added in to regulate pH value and a fermentation medium is obtained, and well-known preserving strain, micrococcus luteus ACCC11001, is selected as liquid fermentation strain and is fermented under certain fermentation conditions. The tobacco flavors after fermentation culture not only can maintain characteristic fragrance contained in original flavors, but also can ensure auxiliary fragrance obtained by fermentation to combine perfectly with the characteristic fragrance, and compared with the original flavors, the grade of the fragrance is improved obviously and the fragrance smells more elegant. The production process of the invention is simple, convenient and easy for extension, thus being provided with greater development and application value.

The invention discloses a novel process for processing chicken manure through aerobic fermentation. A high-efficiency, green chicken manure fermented bio-organic fertilizer is developed by using a rapid fermentation process of mixed bacteria solids. The compound microbial agents include bacillus coagulans, bacillus licheniformis, thermophilic heterotrophic ammonia-oxidizing bacteria, aspergillus niger and the like. The process adopts multi-bacterium mixed fermentation, through the combination of different metabolic abilities, the complex metabolic effects that are difficult to complete by a single strain can be completed, through the synergistic fermentation between microorganisms, the effective conversion of chicken manure is realized, the health indexes meet the national standards, and the product maturity and growth promotion indexes are significantly improved. The fermentation process is simple and efficient, has good deodorizing and sterilizing effect, has low production cost, andis easy for industrial large-scale production.

The invention discloses a tremella fuciformis strain which is screened out. The tremella fuciformis strain is named as XY and is preserved in China General Microbiological Culture Collection Center, apreservation date is April 25, 2019, and a preservation number is CGMCC No.17675. The invention further provides application of the tremella fuciformis strain in tremella polysaccharide production. Amain process of using the tremella fuciformis strain for producing tremella polysaccharides includes the steps: a seed solution prepared from CGMCC No.17675 is used for inoculating a solid-state fermentation culture medium, and fermentation is conducted to obtain a fermentation broth. High-molecular-weight polysaccharides with the molecular weight of 1000-1500 kDa and low-molecular-weight polysaccharides with the molecular weight of 100-300 kDa can be prepared, a total yield can achieve 15-25 g/L, the protein content is smaller than 0.3%, compared with a liquid-state fermentation method, theprocess of using the tremella fuciformis strain for producing the tremella polysaccharides has significant cost advantages, and therefore the tremella fuciformis strain has good application prospectsin industrial production of the tremella polysaccharides.

The invention discloses a preparation method of composite microbial fertilizer and prepared composite microbial fertilizer. The preparation method comprises a yeast pre-fermentation step, a compositestrain fermentation step and a post-fermentation treatment step. In the invention, a straw composition is creatively pre-fermented with yeast, and metabolite contained in a fermentation product is suitable for fermentation of a composite flora. The composite microbial fertilizer has wide raw material sources, mild fermentation conditions and strong vigorous species, and after long-term use, the soil micro-ecological environment can be significantly improved, the soil fertility is improved, and the purposes of increasing crop yield and increasing efficiency are achieved.

The invention relates to a compound strain-fermented goose feed and a preparation method thereof. The invention aims to provides the compound strain-fermented goose feed and the preparation method thereof to solve the problems that the original microbial flora balance state in the intestinal tract of an animal is broken and the immunity of the animal is damaged. The feed specifically comprises fermented soybean meal, silkworm chrysalis meal, plasma protein powder, earthworm powder or shrimp shell powder, a green feed, fermented astragalus residues, bentonite, stone powder, choline chloride, calcium hydrogen phosphate, sodium selenite, a vitamin B group, blueberry pomace, fermented whole-plant corn silage and fine river sand. The method specifically comprises the following steps: preparing the fermented soybean meal, the fermented astragalus residues, the green feed, the blueberry pomace, the plasma protein powder and the silkworm chrysalis meal, conducting mixing and the like. The method is applied to the field of preparation of goose feeds.

The invention relates to a method for preparing cycloastragenol through microorganism mixed fermentation, transformation and degradation of astragaloside iv. Two strains of fusarium proliferatum 0821(CGMCC NO.16482 ) and Fusarium verticillioides 0213 (CGMCC NO.16481 ) which are screened out are used, the astragaloside iv is used as a substrate, and through mixed fermentation of the two strains, the cycloastragenol is prepared. A technique for mixed fermentation with two microorganisms is adopted to degrade the astragaloside iv so as to obtain the cycloastragenol high in purity. The technological flow is simple, the cost is low, and the entire technological process is performed at normal temperature and normal pressure. The required equipment condition is low, the method is suitable for mass production, the conversion rate of the final cycloastragenol is as high as 80% or above, and the purity is as high as 95% or above.

The invention provides a method for preparing a biological pesticide by utilizing tomato stems and leaves, and is characterized in that the method comprises the following steps: raw material preparation, fermentation, and post-treatment of fermentation liquid. The method has the benefits that the tomato planting wastes, namely tobacco stems and leaves, are used as raw materials, and a stock solution of the fermentation liquid is directly sprayed onto plants for insect killing, bacteria inhibition and plant protection without the need to dry, pulverize, extract and concentrate the materials; atthe same time, the extraction of tomatidine by fermentation is more efficient than the extraction of tomatidine by directly using water, alcohol and acid water; the acidic condition of the fermentation liquid enables tomatin to be converted into the tomatidine, which is less in toxicity; sugar does not need to be removed after the fermentation is finished, and can be used as a plant nutrient andadhesive to facilitate reagent attachment and prevent evaporation of water; in addition, the fermentation reaction conditions are mild, both manpower and material resources are saved, no additional alcohol and acid are needed to be added, the cost is reduced, and no pollution is caused to the environment.

The invention provides a method for obtaining chlorogenic acid by fermenting leaves of Acer truncatum. The method uses fresh leaves of Acer truncatum as raw materials and utilizes microorganisms suchas Bacillus to ferment the extract of leaves of Acer truncatum, and fully utilizes chlorogenic acid precursor substance in leaves of Acer truncatum to carry out biological transformation in the fermentation process, thereby accumulating chlorogenic acid in large quantities. The method comprises the step: extracting Acer truncatum leaves by breaking walls, preparing fermentation culture medium, inoculating the strain into fermentation culture medium for culture, and centrifuging the supernatant after fermentation to obtain chlorogenic acid for separation and purification. The fermentation culture medium is composed of 10-20g of sucrose, 10-20g of ammonium chloride, 0. 01-0.02g of cop sulfate, 200-400g of maple syrup and 1000mL of water. The method has the advantages of low cost, high yield,high purity, good activity, simple operating and being suitable for industrial production.

The invention discloses a method for improving the oxidation resistance of abalone biological products, and belongs to the technical field of marine organisms. An abalone polypeptide biological product with high in-vitro oxidation resistance is developed by taking abalone protein as a raw material and integrating a modern biological separation technology, a biological enzymolysis technology and amixed bacteria microbial fermentation technology, and mixed bacteria aerobic fermentation strains mainly comprise candida utilis and bacillus subtilis. The obtained abalone polypeptide has strong oxidation resistance capacity, the extracting solution can be subjected to concentration, adsorption drying, alcohol precipitation or resin purification and other treatment according to application ways,and efficient utilization in the fields of health care, foods, medicine and the like is met. The process technology is simple and efficient, and the obtained product is high in oxidation resistance and free radical scavenging capacity, high in stability, low in production cost and easy for industrial large-scale production.

The invention discloses a bio-organic fertilizer capable of efficiently preventing and controlling rice diseases and a preparation method of the bio-organic fertilizer, and belongs to the technical field of the bio-fertilizers. The bio-organic fertilizer integrates the technologies of comprehensive utilization of modern bacteriostatic activity substances and microbial fermentation of mixed bacteria, excrements of livestocks, straw, Chinese herb materials and carrageenan oligosaccharide are fermented through a synergistic effect of microorganisms, the synergistic interaction of materials is given into full play, and the efficient and green fermented bio-organic fertilizer is researched and developed. The bio-organic fertilizer is capable of efficiently inhibiting diseases such as false smutof rice, banded sclerotial blight, rice blast, bacterial leaf blight and the like in the planting of the rice, changing the defect that a chemical bactericide is mainly used in the prevention of therice diseases in the prior art and reducing the drug residue in the planting process of the rice, has an obvious effect of increasing the yield, is simple in production process, relatively low in production cost, and can obviously improve the food safety and the planting benefit of the rice.