38results about How to "Mild fermentation conditions" patented technology

The invention discloses a method for preparing ellagic acid by solid fermentation with granatum as a raw material and belongs to the field of medicines and health-care products. In the method, solid fermentation is performed by using a culture medium which takes granatum as a main ingredient and by using Aspergillusniger 3.316 as a fermenting bacterial strain. The conditions for the solid fermentation of the granatum include: the pH value is between 5 and 7; and the granatum is initially moisturized by water by 47 to 60 percent, 0.5-percent NH4NO3 is added into a moisturizing agent as an N source, and fermentation is performed at 30 DEG C for 5 to 7 days. The fermented product is purified and dehydrated to produce the high-quality ellagic acid product. The granatum serving as a raw material is cheap, and the high yield and purity of the ellagic acid product are ensured; and the method has an outstanding cost advantage and is suitable for large-scale production.

The invention belongs to the technical field of tobacco processing and relates to a method for preparing basic material of a tobacco thin sheet, namely tobacco stem cellulose through the microbial solid fermentation process. Microbes including high-yield prolease, pectolase, ligninase, amylase and other enzymes are selected, and tobacco stems, tobacco smalls and crumbled tobacco are taken as raw materials for preparing microbial seeds, the prepared microbial seeds are inoculated into the tobacco stems for performing solid fermentation, soaking the tobacco stems after fermentation in buffer solution, further performing enzymolysis for degrading the tobacco stems after the enzymolysis, proteins, lignin, pectic substances and other macromolecular substances into micromolecular substances which are easy to be separated from cellulose type substances in the tobacco stems, and further stirring, pulping, filtering and separating for getting water-soluble extract and water-insoluble celluloseof the tobacco stems. The method has the advantages of being simple in process, saving energy, reducing equipment investment and improving the synthetic quality of the tobacco thin sheet, and can greatly reduce the residual quantity of the proteins, pectin, starch and other macromolecules in the tobacco thin sheet.

The invention relates to comprehensive utilization of an aquatic seasoning and an aquatic waste, in particular to a method for fast brewing shrimp oil by taking shrimp heads and shrimp shells as raw materials, belonging to the field of aquaculture. The method for brewing the shrimp oil by taking the shrimp heads or / and the shrimp shells as the raw materials and adding salt comprises the following steps of: (1) crushing the shrimp heads or / and the shrimp shells, and then adding the salt and proteases to form fermentation materials; (2) fermenting for some time by keeping temperature not higher than 60 DEG C, termly stirring in the fermentation process, and controlling a pH value between 7.0 and 8.2; and (3) after fermentation is finished, separating fermentation liquor from fermentation residuals to obtain the fermentation liquor. The invention reduces the production cost by substituting the shrimp heads and the shrimp shells for shrimps; the brewed shrimp oil has strong and comfortable odour without stinking smell, is suitable for being used as a food seasoning, has various nutrient components and is beneficial to the regulation of the balanced intake of nutrient elements and the human health.

The invention discloses a bioconversion method for series steroid compound 19-position hydroxylation and belongs to the field of chemistry and biology. The method comprises the steps as follows: a steroid compound and iron salt are added to a liquid culture medium containing bioconversion strains for culture, wherein the bioconversion strains contain a strain with a steroid compound 19-position hydroxylation function and a heterologous expression strain containing a steroid compound 19-position hydroxylation gene; and the steroid compound contains cortodoxone, 17-position and / or 21-position hydroxyl protected cortodoxone as well as other modification of steroid frameworks on the basis of the defined compounds. The method can be used for efficient steroid 19-position hydroxylation to obtainseries steroid hydroxylated products. The method solves the problems of low yield and poor selectivity of 19-posiiton hydroxylated products of the steroid compounds, used raw materials are cheap andavailable, the reaction condition is mild, the substrate universality is good, the yield is high, the preparation process is simple, and the method has very high industrial application values.

In the present invention, a Tremella fuciformis strain (Tremella fuciformis) was screened out, the strain name is XY, and it was deposited in the General Microbiology Center of the China Microbiological Culture Collection Management Committee. The preservation date is April 25, 2019, and the preservation number is CGMCC No.17675. The present invention also provides the application of the above Tremella fungus strains in the production of Tremella polysaccharides, the main process of which is to insert the seed liquid prepared from CGMCC No. 17675 into a solid-state fermentation medium for fermentation to obtain a fermentation liquid. It can prepare high-molecular-weight polysaccharides with a molecular weight of 1000-1500kDa and low-molecular-weight polysaccharides with a molecular weight of 100-300kDa. The total output can reach 15-25g / L, and the protein content is less than 0.3%. Compared with the liquid fermentation method, it has a significant cost advantage. Tremella It has a good application prospect in the industrial production of polysaccharides.