This invention belongs to the field of
biomedical technology, specifically disclosing a method for extracting and applying fermented sword bean oil. The method involves first activating
Bacillus subtilis, then inoculating it onto sword bean
powder for
solid-state
fermentation. After
drying and pulverizing the fermented material, extraction is performed using an extraction
solvent on a
shaker and followed by
filtration. The filtrate is then evaporated under vacuum to remove the
solvent, yielding fermented sword bean oil. This method disrupts the
cell walls of
sword beans, promoting oil release and increasing the oil yield. After
fermentation, the content of polyunsaturated fatty acids in the oil increases to 25.763±0.663%, with the addition of pentadecenoic acid and
erucic acid, as well as
DPPH and
ABTS. + The free radical
scavenging ability and iron
ion reducing ability are significantly enhanced, resulting in improved
antioxidant activity. The
process conditions of this invention are mild, the process is simple, the
energy consumption is low, and it is easy to scale up. The resulting
oil can be used in the food, health product, and pharmaceutical industries.