A ganoderma mushroom health care wine is mixed by Junchen medicinal wine and assistant and guide medicinal wine with volume ratio as 2:1-1.5, the Junchen medicinal wine is made by Jun medicine: ganoderma, mushroom, milkvetch, sealwort, red enter, saline cistanche, rhizome of davallia, reticulate millettia, Chen medicine: cultivated land, yam rhizome, songaria cynomorium herb, boxthorn fruit, shizandra, radix polygonati officinalis, fleece-flower root infused by pure rice wine with 40-50 degree, assistant medicinal wine is composed by assistant medicine: eucommia bark, himalayan teasel root, moghania, largeleaf gentian root, guide medcine: brazilwood, red sage root, ovateleaf holly bark, licorice root, mulberry, white paeony root infused by pure rice wine with 40-50 degree; the preparing method including: preparing material, weighting each component of Junchen medicine, assistant and guide medicine by proportion respectivelly, pre-preparing specially made medicine, immersing Junchen medicine medicinal wine, assistant and guide medicinal wine, preparing package. The wine of the invention without any chemicals, essence and colorant has good function to gas, blood, liver, kidney, heart and brain to improve immune ability of body, the preparing method is simple and reasonable.