Culture medium used for separating penicillium

A culture medium and Penicillium technology, applied in the direction of microorganism-based methods, fungi, microorganisms, etc., can solve the problems of increased workload, long cycle, contamination of Penicillium growth, etc., easy to achieve colony shape, short cycle, easy to obtain Effect

Inactive Publication Date: 2015-10-07
NINGXIA ACADEMY OF AGRI & FORESTRY SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these media have deficiencies in varying degrees. For example, the Penicillium colonies on the Bengal red medium are small and difficult to identify. It is important to know that the Penicillium colonies cultured on the medium are also suitable for testing whether a medium is suitable. Indicators, if the cultured colonies are not easy to identify, they must be observed under a microscope, which will greatly increase the workload; the cultivation period of CA and CYA medium is longer, and PDA medium is easy to grow other miscellaneous bacteria , will inhibit and contaminate the growth of Penicillium, etc.

Method used

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  • Culture medium used for separating penicillium
  • Culture medium used for separating penicillium
  • Culture medium used for separating penicillium

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Embodiment Construction

[0016] A medium for isolating Penicillium, the content of each component is: glucose 15g, yeast extract 5g, Bengal red 0.033g, sodium nitrate 3g, magnesium sulfate 0.5g, potassium dihydrogen phosphate 1.5g, agar 12g, Distilled water 1000ml, streptomycin 30mg.

[0017] Its preparation method is: weigh the above-mentioned content of Bengal Red, sodium nitrate, magnesium sulfate and potassium dihydrogen phosphate, add it into a beaker filled with 10ml of distilled water and stir well, add 1000ml of distilled water into the heating container, and add a little more to prevent evaporation loss For the dropped water, add 15g of glucose after the distilled water boils, add 5g of yeast extract and stir the solution in the beaker, finally add 12g of agar powder, stir while adding, and finally sterilize by high pressure steam for 15min, add streptomycin when pouring the plate 30 mg.

[0018] The screening comparative test of the present invention and other culture medium is as follows: ...

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Abstract

A culture medium used for separating penicillium comprises glucose, yeast cream, rose bengal, sodium nitrate, magnesium sulfate, monopotassium phosphate, agar, streptomycin and distilled water. The culture medium is named as a yeast cream glucose agar culture medium or YDA for short. Components of the culture medium are all common chemical agents, so that the culture medium is low in cost and easy to obtain. Penicillium cultured by adopting the culture medium is short in period and easy-to-identify in colonial shape, and time is saved for strain selecting at the initial stage of penicillium classifying. In addition, compared with other selective culture media, the culture medium has the advantages that types of selected penicillium are various, and the culture medium is a selective culture medium excellent in comprehensive penicillium separating effect.

Description

Technical field: [0001] The invention relates to the technical field of selective medium, in particular to a medium for isolating Penicillium. Background technique: [0002] Penicillium (Penicillium) fungi (hereinafter referred to as Penicillium) is a common fungus in nature. Production and life are closely related, and it has very important economic value in itself. The research on Penicillium in foreign countries is relatively early and comprehensive, while domestic research is relatively few, and no one has systematically involved in the research on Penicillium in Ningxia. Therefore, the investigation, classification and identification of Penicillium in Ningxia can provide a theoretical basis for enriching and perfecting the taxonomy of Penicillium, and the study of Penicillium is of great significance both in theory and in practical application. [0003] And the selection of medium plays a vital role in the separation and classification process of Penicillium, there ar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/80
CPCC12N1/14
Inventor 沈瑞清安婧婧
Owner NINGXIA ACADEMY OF AGRI & FORESTRY SCI
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