Composite microbial inoculant for treating grease wastewater, and application and use method thereof

A compound microbial agent and wastewater technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of not being able to provide strain mixing optimization methods, and not being able to clarify the role, etc., to achieve high industrial application value , Improving the efficiency of wastewater treatment and reducing the cost of wastewater treatment

Inactive Publication Date: 2015-02-04
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is a strong synergistic effect between the strains in the mixed bacterial agent, and the degradation effect on wastewater is better than that of a single strain. Compared with the early research, the applicability and degradation abi...

Method used

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  • Composite microbial inoculant for treating grease wastewater, and application and use method thereof
  • Composite microbial inoculant for treating grease wastewater, and application and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: the preparation method of the composite microbial bacterial agent of processing grease waste water

[0018] The preparation method of the compound microbial bacterial agent for treating oily wastewater is as follows: respectively take Pseudomonas cepacia, Micrococcus, Bacillus licheniformis, Bacillus amyloliquefaciens, Penicillium expanses and Yarrowia lipolytica to inoculate the actual oily wastewater after sterilization. cultured in an environment of 180rpm and 30°C, and tested the OD of the bacterial solution every 3 hours 600 , to each strain OD 600 Both are 0.30±0.05. Mix 1mL of Pseudomonas cepacia, 1mL of Micrococcus, 2mL of Bacillus licheniformis, 2mL of Bacillus amyloliquefaciens, 2mL of Penicillium dilatans and 2mL of Yarrowia lipolytica , forming a composite microbial agent.

Embodiment 2

[0019] Embodiment 2: the preparation method of the activator that strengthens grease degradation ability

[0020] The preparation method of the compound microbial bacterial agent for treating oily wastewater is as follows: respectively take Pseudomonas cepacia, Micrococcus, Bacillus licheniformis, Bacillus amyloliquefaciens, Penicillium expanses and Yarrowia lipolytica to inoculate the actual oily wastewater after sterilization. cultured in an environment of 180rpm and 30°C, and tested the OD of the bacterial solution every 3 hours 600 , to each strain OD 600 Both are 0.30±0.05. Mix 3 mL of Pseudomonas cepacia, 3 mL of Micrococcus, 1 mL of Bacillus licheniformis, 1 mL of Bacillus amyloliquefaciens, 1 mL of Penicillium dilatans and 1 mL of Yarrowia lipolytica , forming a composite microbial agent.

Embodiment 3

[0021] Embodiment 3: The experiment of adding the compound microbial bacterial agent to the oily waste water grease trap

[0022] A grease trap for treating oily wastewater with an effective volume of 1.5m 3 . According to the dosage of 0.01% of the wastewater volume, take 150mL of oily wastewater, inoculate the compound microbial agent after sterilization, place it in an environment of 180rpm and 30°C for 72 hours, and then add the oily wastewater containing the compound microbial agent to the oil separator In the pool, after that, add once every 8 days, the dosage is the same as above.

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Abstract

The invention discloses a composite microbial inoculant for treating grease wastewater, and application and a use method thereof. The composite microbial inoculant is compounded from the following components in percentage by volume: 10-30% of Pseudomonas cepacia, 10-30% of micrococcus, 10-20% of Bacillus licheniformis, 10-20% of Bacillus amyloliquefaciens, 10-20% of Pexpansum and 10-20% of Yarrowia lipolytica. The OD600 of each strain is 0.30+/-0.05. Under the action of the composite microbial inoculant for treating grease wastewater, the ecological niche in the wastewater system is more abundant, different types of microbes closely cooperate to perform more efficient metabolism, thereby enhancing the wastewater treatment efficiency and lowering the treatment cost. The composite microbial inoculant is simple in manufacturing technique and use mode, has obvious enhancement actions on treatment effect of the high-concentration grease wastewater, and has very high industrialization application value.

Description

technical field [0001] The invention relates to a compound microbial bacterial agent for treating oily wastewater and its application and application method. Background technique [0002] Wastewater from slaughterhouses, dairy processing plants, restaurants, etc. contains a large amount of non-biodegradable oils and fats. High-concentration oily wastewater discharged into the surrounding water without treatment will form an oil film on the water surface, hinder oxygen transmission, cause water hypoxia, and threaten the survival of aquatic animals and plants. In anaerobic biological treatment, grease will accumulate on the sludge surface, hinder the transmission of soluble pollutants, and reduce the degradation efficiency of microorganisms on pollutants; a large amount of grease adheres to the sludge, reducing the sedimentation performance of the sludge, resulting in difficulty in sedimentation, resulting in A large amount of activated sludge is lost, which will eventually a...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C12N1/14C02F3/34C12R1/38C12R1/265C12R1/10C12R1/07C12R1/83C12R1/645
CPCC12N1/20C02F3/34C02F3/347C02F2101/34C12N1/14C12N1/16
Inventor 梁志伟刘国防王存豹何李宜
Owner ZHEJIANG UNIV
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