Penicillium oxalicum (fungus) capable of efficiently dissolving phosphorus, culture method and applications thereof

A technology of Penicillium oxalate and phosphate-solubilizing fungi is applied in the fields of application, fungi, chemical instruments and methods, etc., which can solve the problems of nutrient imbalance, nitrogen and phosphorus accumulation, etc. The effect of fruit and vegetable production

Inactive Publication Date: 2016-07-06
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Facility cultivation has increasingly become the main way for people to obtain high-yield, high-quality, and high-efficiency vegetables. However, in recent decades, in order to pursue higher economic benefits, people have not hesitated to apply excessive fertilizers to the soil, especially nitrogen fertilizers and phosphorus fertilizers. This will lead to the accumulation of nutrients in the soil of the facilities, the serious imbalance of nutrients, and the serious accumulation of nitrogen and phosphorus

Method used

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  • Penicillium oxalicum (fungus) capable of efficiently dissolving phosphorus, culture method and applications thereof
  • Penicillium oxalicum (fungus) capable of efficiently dissolving phosphorus, culture method and applications thereof
  • Penicillium oxalicum (fungus) capable of efficiently dissolving phosphorus, culture method and applications thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0027] The soil was passed through a 2mm sieve, and the water content and pH value were measured. Soil samples were cultured in 250mL Erlenmeyer flasks, each bottle was added with 100g of soil, and each bottle was added with 10mL of fungal spore suspension, the concentration of spore suspension was 1×10 7 CFU / ml, the control group was added with an equal volume of sterile deionized water. Add an appropriate amount of urea (0.36g / kg soil) to the soil to replenish water so that the total water content in the soil is maintained at 18%-20%. Finally, the triangular flask was sealed with parafilm. Placed in a biochemical incubator at 28°C. Set up a treatment group (add bacteria solution, set 4 repetitions) and a control group (do not add bacteria solution, also set 4 repetitions). Samples were taken on day 0, day 3, day 5, day 10, day 15, day 20, day 30, day 45, and day 60 (the control group also had to be sampled regularly). Determination of available phosphorus content in the ...

Embodiment 2

[0029] pot experiment design

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Abstract

The invention discloses penicillium oxalicum (fungus) capable of efficiently dissolving phosphorous. The preservation number of the strain is CGMCC No.11126. The strain is separated from soil in a booth, which has been cultivated for many years. The culture medium of penicillium oxalicum is prepared from the following components by weight: 10 grams of glucose, 0.5 gram of ammonium sulfate, 0.3 gram of sodium chloride, 0.3 gram of potassium chloride, 0.03 gram of ferrous sulfate heptahydrate, 0.03 gram of manganese sulfate tetrahydrate, 0.3 gram of magnesium sulfate heptahydrate, 5 grams of tricalcium phosphate, and 20 grams of agar. The culture temperature is 30 DEG C, and the culture pH value is 7.0 to 7.2. The provided penicillium oxalicum has a very strong phosphorus dissolving function, can be used to prepare a soil phosphorus activator for improving the phosphorus utilization rate, and is capable of successfully positioning phosphorus to the plant roots, thus the using amount of phosphorus containing fertilizer is reduced, and the output of fruit and vegetable is increased.

Description

technical field [0001] The invention relates to the field of bacterial strains, in particular to a high-efficiency phosphorus-solubilizing fungus Penicillium oxalicum and its cultivation method and application. Background technique [0002] Facility cultivation has increasingly become the main way for people to obtain high-yield, high-quality, and high-efficiency vegetables. However, in recent decades, in order to pursue higher economic benefits, people have not hesitated to apply excessive fertilizers to the soil, especially nitrogen fertilizers and phosphorus fertilizers. This will lead to the accumulation of nutrients in the soil of the facilities, the serious imbalance of nutrients, and the serious accumulation of nitrogen and phosphorus. However, when the soluble phosphorus fertilizer is applied to the soil, part of it can be absorbed and utilized by plants, while the other part reacts with soil components and moves out of the soil phase, becoming an insoluble phosphate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C09K17/14C12R1/80C09K101/00
CPCC09K17/14C09K2101/00C12N1/14C12N1/145C12R2001/80
Inventor 依艳丽尚双华
Owner SHENYANG AGRI UNIV
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