Paecilomyces hepialid mycelium and preparation method thereof

A technology of Paecilomyces spp. and mycelium, applied in botany equipment and methods, horticulture, applications, etc., can solve the problems of not effectively controlling the medium and not observing the mycelium

Inactive Publication Date: 2014-06-11
LANZHOU KELIN BIO PHARMA
View PDF7 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And the current culture of mycelium is because the second stage did not effectively control the problem of miscellaneous bacteria in the medium, resulting in being eaten by other faster-growing fungi during the cultivation process and turning into golden yellow mushrooms. The result is: the mycelium cannot be observed with a 600 times ordinary microscope

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Paecilomyces hepialid mycelium and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0017] 1. Dissect the parasite and separate the strain into the test tube.

[0018] 2. Cultivate the slant bacteria on the FDA slant medium at constant temperature for 7 days, and separate the miscellaneous bacteria while cultivating and complete the rejuvenation process.

[0019] 3. The strains were inserted into shake flasks containing culture medium and cultured with shaking for 48 hours.

[0020] 4. Preparation of solid medium: use 95% naked oats and 5% peptone to form a solid medium; then cook and sterilize the solid medium at a high temperature of 120°C, take it out of the pot and cool it, and put it in the shaker flask under sterile conditions The cultured Cordyceps sinensis liquid is inoculated on the culture medium according to the weight ratio of 10%, fermented, and cultured at 20-22°C for 7-10 days; the obtained white mycelium is dried at 50-60°C to obtain Cordyceps sinensis Mycelium. The preparation method of the Cordyceps sinensis mycelium of the present inventi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a paecilomyces hepialid mycelium and a preparation method thereof. The mycelium is obtained by cultivating paecilomyces hepialid via a solid culture medium. The preparation method technically includes: dissecting fresh cordyceps sinensis polypide, separating a paecilomyces hepialid culture in the cordyceps sinensis polypide, cultivating and separating hetero-cultures out, preparing the solid culture medium for sterilizing, inoculating 10% by weight of cultivated and rejuvenated liquid culture on the culture medium for fermentation, and cultivating in an aseptic condition at the temperature of 20-22DEG C for 7-10 days to obtain the mycelium. The mycelium prepared by the preparation method is equal to natural cordyceps sinensis in aspects of nutrition and medical values. The paecilomyces hepialid mycelium is characterized in that under an ordinary 300-600-multiplication optical microscope, the mycelium is 16-40 micrometers in visible length and 3-5 micrometers in root width. The mycelium mainly comprises adenosine which cannot be dissolved by water and can be dissolved by ethyl alcohol.

Description

technical field [0001] The invention belongs to a kind of Cordyceps sinensis mycelium, which is a biological technology product. Products are widely used in medicine, food and health food. Background technique [0002] Cordyceps sinensis is neither a worm nor a grass, it is a complex of fungi and worms. On the Qinghai-Tibet Plateau at an altitude of 3,500 to 6,000 meters in China, there lives a cold-resistant insect called the bat noctuid. Its adult period is only 4 to 12 days, while its larval period is 680 to 940 days. A fungus called Cordyceps sinensis (scientific name: Paecilomyces bat moth or Trichosporum bat moth) is active at almost the same altitude. The larvae of a bat noctuid, Cordyceps sinensis, drill into the worm body to parasitize it. [0003] Bat moth larvae are usually infected at the end of autumn and early winter, and the fungus that invades the insect body develops into sclerotia about a month later. At the same time, the larvae of the bat moth stopped...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 斯理想
Owner LANZHOU KELIN BIO PHARMA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap