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Biological control method for fruit and vegetable penicilliosis pathogens

A control method and technology for pathogenic bacteria, which are applied in the fields of chemicals for biological control, botanical equipment and methods, biocides, etc., and can solve problems such as drug-resistant strains, pesticide residues, and environmental pollution.

Inactive Publication Date: 2020-10-30
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemical fungicides such as imazalil and thiabendazole are commonly used in actual production to control postharvest diseases of fruits and vegetables. However, long-term and large-scale use will cause many problems such as drug-resistant strains, pesticide residues, and environmental pollution. Looking for safety , Efficient disease prevention and control measures are an urgent problem to be solved in production
[0004] Heptanal is a safe and non-toxic plant secondary metabolite, which can be obtained by distillation from plants such as lemon, bitter orange, rose, and hyacinth. Raw materials, there is no literature report on the antibacterial effect of heptanal

Method used

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  • Biological control method for fruit and vegetable penicilliosis pathogens
  • Biological control method for fruit and vegetable penicilliosis pathogens
  • Biological control method for fruit and vegetable penicilliosis pathogens

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Example of a spore germination assay

[0023] Spread the sterilized medium on a glass slide and spread 20 μL evenly at a concentration of 1×10 6 cfu / mL Penicillium italiensis spore suspension. Place a piece of sterilized circular filter paper with a diameter of 20 mm in the center of the Petri dish cover, and use a pipette to pipette heptanal onto the filter paper, so that the final concentrations are 0, 5, 7.5, 10, 12.5, 15 μL / L, respectively. Heptanal was not added as a control group. After rapidly sealing the petri dish and culturing in a 25°C incubator for 10 hours, the spore germination was observed under a microscope, and the inhibition rate of spore germination was measured and calculated. Each treatment was repeated 3 times, 3-4 fields of view were counted for each repetition, and each field had about 30-40 spores, that is, at least 100 spores were counted for each repetition.

[0024] The results showed that different concentrations of heptanal co...

Embodiment 2

[0025] Example 2: Mycelial Growth Assay Example

[0026] (1) Inoculate 200 μL of spore suspension on 20 mL of medium, spread evenly on the applicator, invert at 26°C for about 36 hours until white young mycelium is evenly visible, prepare a fungus cake with a 9mm diameter hole punch, and place the fungus cake Put it in the center of the new medium, cover the petri dish with sterile filter paper and add heptanal dropwise, and quickly seal it with a parafilm, so that the concentration of heptanal in the plate is 0, 2, 4, 8, 16 μL / L, with no Heptanal was added as a control group. The plate was cultured at 25°C, and the colony diameter of Penicillium italica was measured by the cross method after 0, 24, 48, and 72 h.

[0027] (2) 200 μL of spore suspension was inoculated into 50 mL of liquid medium, cultured at 26±2°C, 120 r / min shaker for 36 h to obtain young mycelium, and collected by sterile filtration, accurately weighed 0.5 g of wet weight mycelium and inoculated into the me...

Embodiment 3

[0029] Example 3: Measurement Example of Cell Membrane External Conductivity

[0030] Spread a layer of sterilized cellophane on the cooled and solidified 20mL PDA medium, then inoculate 200μL of spore suspension, spread evenly on the applicator, invert at 26°C for about 18h until the white young mycelium is evenly visible, and then add 0, 8, and 16 μL / L of heptaldehyde were fumigated, and the treated mycelia were obtained by inverting culture at 26° C. for 6 h for later use. Weigh 0.5 g of mycelium in a 50 mL clean centrifuge tube, resuspend in 20 mL of double-distilled water, take the mycelial solution without heptaldehyde fumigation as the control group, and measure the conductivity of the solution at 4 h with a conductivity meter.

[0031] Depend on Figure 4 It can be seen that the conductivity of the solution outside the membrane of Penicillium italy increased with the increase of the fumigation concentration under the condition of heptanal fumigation. The L treatment ...

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Abstract

The invention discloses an application of heptanal as a penicillium italicum inhibitor, and belongs to the technical field of food preservation. The invention also discloses a penicillium italicum inhibitor and a biological control method of fruit and vegetable penicilliosis pathogens. The method comprises the step of treating fruits and vegetables with heptanal. Test results show that heptanal enables penicillium italicum spores not to germinate, hypha growth is inhibited, permeability of cell membranes is improved, and proteins in the membranes is promoted to seep out. The natural plant extract heptanal is used as an antibacterial agent for controlling the pathogen penicillium italicum of postharvest penicilliosis of the fruits and vegetables, and compared with a common chemical prevention and treatment method, the biological control method is harmless and pollution-free to human bodies and the environment.

Description

technical field [0001] The invention belongs to the technical field of food preservation, and in particular relates to a biological control method for the pathogenic bacteria of penicillosis in fruits and vegetables. Background technique [0002] Fruits and vegetables play an extremely important role in people's lives. They are not only rich in vitamins, minerals, trace elements and other nutrients needed by the human body, but also rich in cellulose, which play an important role in human health. With the development of the economy and the improvement of people's living standards, the output and trade of fruits and vegetables in my country continue to increase. However, most fruits and vegetables are rich in nutrients and large in water content, so they are easily infected by pathogenic fungi and rot and deteriorate during post-harvest storage, transportation and sales. Among them, Penicillium italy is an important pathogenic fungus that causes postharvest rot and deteriora...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N35/02A01N25/18A01P3/00
CPCA01N25/18A01N35/02
Inventor 左盼盼杨书珍
Owner HUAZHONG AGRI UNIV
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