Dicoumarin derivative and preparation method and application thereof

A technology of dicoumarin and derivatives, applied in biochemical equipment and methods, botany equipment and methods, methods based on microorganisms, etc., can solve problems such as dicoumarin compounds that have not been seen yet, and achieve broad application prospects Effect

Active Publication Date: 2017-11-28
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The compound described by this patented formula works very well against various types of molds that cause damage or reduce their ability for crops such as rice blast disease (rice sheath germination). This makes it possible to use these compositions more effectively than previous methods like pesticides because they are easier to produce at larger scales compared to other chemicals.

Problems solved by technology

The technical problem addressed by this patented research relates to developing an efficient chemical pesticide called bifurarone (BFI) without causing environmental harm while still producing useful bioactive products like other types of natural molecules.

Method used

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  • Dicoumarin derivative and preparation method and application thereof
  • Dicoumarin derivative and preparation method and application thereof
  • Dicoumarin derivative and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Dicoumarin derivatives were prepared by the following steps:

[0035] (1) Carry out strain culture to bitter threshold blue (Myoporum bontioides A.Gray) endophytic fungus Aspergillus clavatus (Aspergillus clavatus) R7 earlier in strain culture medium, used strain culture medium is to contain glucose 1.0% (percentage by weight, The same below), yeast extract 0.02%, peptone 0.01, agar 1.0%, sodium chloride 0.2%, and the rest is water. When used, it is made into a test tube slope, and the above-mentioned strains are cultivated at 28°C for 10 days;

[0036] (2) Carry out fermentation culture to Aspergillus clavatus (Aspergillus clavatus) R7 again, the fermentation medium used is to contain rice 70% (percentage by weight, the same below), sodium chloride 1.0% and water balance, stand still at room temperature 25 ℃ Cultured for 30 days.

[0037] (3) The strain fermented in step (2) is extracted 3 times with ethanol-water mixed solution (the volume ratio of ethanol and water...

Embodiment 2

[0039] Dicoumarin derivatives were prepared by the following steps:

[0040] (1) Carry out strain culture to endophytic fungus Aspergillus clavatus (Aspergillus clavatus) R7 in strain culture medium earlier, used strain culture medium is to contain glucose 3.5% (percentage by weight, the same below), yeast extract 1.5% %, peptone 1.5%, agar 2.5%, sodium chloride 2.0%, and the rest is water. When used, it is made into a test tube slope, and the above bacteria are cultured at 28°C for 3 days;

[0041] (2) Carry out fermentation culture to Aspergillus clavatus (Aspergillus clavatus) R7 again, the fermentation medium used is to contain rice 30.0% (percentage by weight, the same below), sodium chloride 3.0% and water balance, static culture at 20 ℃ 60 days;

[0042] (3) The strain fermented in step (2) is leached 4 times with methanol-water mixture (95:5 in volume ratio), concentrated under reduced pressure, extracted 4 times with n-butanol (n-butanol can be extracted with ethyl ...

Embodiment 3

[0044] Dicoumarin derivatives were prepared by the following steps:

[0045](1) Carry out strain culture to endophytic fungus Aspergillus clavatus (Aspergillus clavatus) R7 in the strain culture medium earlier, the strain culture medium used is to contain glucose 2.5% (percentage by weight, the same below), yeast extract 0.5% %, peptone 1.0%, agar 1.5%, sodium chloride 1.5%, and the rest is water. When used, it is made into a test tube slope, and the above bacteria are cultured at 28°C for 3 days;

[0046] (2) Carry out fermentation culture to Aspergillus clavatus (Aspergillus clavatus) R7 again, the fermentation medium used is to contain rice 50.0% (percentage by weight, the same below), sodium chloride 2.5% and water balance, in static culture at 30 ℃ 45 days;

[0047] (3) The strain fermented in step (2) is leached twice with ethanol-water mixed solution (volume ratio is 80:20), after concentrating under reduced pressure, extract twice with n-butanol (n-butanol can use et...

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Abstract

The invention discloses a dicoumarin derivative and a preparation method and application thereof. The molecular formula of the dicoumarin derivative is C22H18O7, wherein the structural formula is as shown in a formula I. The preparation method of the dicoumarin derivative comprises the following steps: (1) carrying out bacterial culture on aspergillus clavatus (Aspergillus clavatus) R7 in a strain medium; and (2) carrying out fermentation cultivation on the strain obtained in the step (1) in a fermentation medium and then extracting by using an ethanol water solution or a methanol water solution, carrying out vacuum concentration, extracting by using an organic solvent and finally further purifying through silica-gel column chromatography, Sephdex LH-20 gel column chromatography and recrystallization to obtain the dicoumarin derivative. The prepared dicoumarin derivative has very strong effects of inhibiting tomato blight, penicillium italicum and gloeosporium musarum and can be used as a microbial bactericide, the raw materials can be produced on a large scale and the application prospect is wide. The formula is as shown in the specification.

Description

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Claims

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Application Information

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Owner SOUTH CHINA AGRI UNIV
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