A kind of Hansenula viticola and its application

A technology of yeast, grapes, applied in the field of microbiology

Inactive Publication Date: 2016-03-23
北京莱万生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Hansenia sporauvarum (Hanseniasporauvarum) is widely distributed in nature, but there is no report on the control of grape gray mold and peach brown rot in China

Method used

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  • A kind of Hansenula viticola and its application
  • A kind of Hansenula viticola and its application
  • A kind of Hansenula viticola and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] Isolation and strain identification of Hansenia sporauvarum Y68 of the present invention

[0079] 1. Sample collection

[0080] Grapes are collected from organic vineyards in China.

[0081] 2. Isolation and screening of strains and antagonistic screening

[0082] Isolate strains from organic fruits, use conventional gradient dilution coating to isolate, use two kinds of media, PDA (containing streptomycin sulfate) and YPD agar (containing streptomycin sulfate), to culture at 28°C respectively, and pick colonies Bacteria with large morphological differences and can be purely cultured were purified and preserved on YPD agar medium, and Botrytis cinerea was used as the target pathogen to conduct preliminary screening and multiple re-screening of antagonistic bacteria, and finally a strain with strong antibacterial activity was obtained. Yeast strain, named Y68.

[0083] 3. Identification of strains

[0084] Morphological, physiological and biochemical characteristics ...

Embodiment 2

[0088] Determination of antibacterial spectrum of Hansenia sporauvarum (Hansenia sporauvarum) Y68 described in the present invention

[0089] Pathogens tested:

[0090] Peach brown rot fungus - Sclerotinia sclerotiorum (Monilinia fracticola (wint.) Rehm);

[0091] Cherry brown rot fungus - Monilinia fracticola (wint.) Rehm;

[0092] Botrytis cinerea Per.exFr, the postharvest botrytis cinerea of ​​apples;

[0093] Penicillium expansum Link, 1809, the postharvest penicillium of apples--Penicillium expansum Link;

[0094] Penicillium expansum Link, 1809;

[0095] Botrytis cinerea -- Botrytis cinerea Per.exFr;

[0096] Botrytis cinerea Per.exFr, Botrytis cinerea Per.exFr;

[0097] The determination of the inhibitory effect on botrytis cinerea was carried out by in vitro plate confrontation method: Botrytis cinerea was selected as the indicator fungus: plate confrontation culture method was used. The specific operation is as follows: Hansenia sporauvarum (Hansenia sporauvarum) ...

Embodiment 3

[0104] Control effect of Hansenia sporauvarum Y68 on postharvest diseases

[0105] Under storage conditions, the control effect experiment of Hansenia sporauvarum Y68 on postharvest diseases of peaches, apples, pears and grapes:

[0106] 1. After the peaches are picked, they are soaked in the Y68 bacterial solution for 45-60 seconds, then air-dried at room temperature, and then stored in the dark at a temperature of 2-4°C and a relative humidity of 90-95%. After storage for 21 days, compared with the control group, the control effect was calculated. When the cell concentration is ≥10 8 When cfu / ml, the control effect is above 80%; when the cell concentration is 10 7 When cfu / ml, the control effect is 50%; when the cell concentration is 10 6 When cfu / ml, the control effect is 40%. The effective concentration of its application is 10 8 cfu / ml.

[0107] 2. After the apples are picked, they are soaked in the Y68 bacterial solution for 45-60 seconds, then air-dried at room tem...

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Abstract

The invention discloses hanseniaspora uvarum; the bacterial strain is Y68; the preservation number is CGMCC No. 8042. The hanseniaspora uvarum Y68 has the inhibiting effect on post-harvest monilinia fructicola of peaches and cherries, post-harvest botrytis cinerea of grapes, tomato botrytis, post-harvest botrytis cinerea of apples, post-harvest penicillium italicum of apples and post-harvest penicillium italicum of pears, and has the inhibiting effect on post-harvest brown rot of peaches, cherry brown rot, grape botrytis, tomato botrytis, post-harvest botrytis of apples, post-harvest penicilliosis of pears and post-harvest penicilliosis of apples. The hanseniaspora uvarum Y68 specifically has obvious inhibiting effect on the botrytis cinerea; the diameters of inhibition zones are all greater than 10 mm.

Description

technical field [0001] The invention relates to Hansenula viticola and its application, belonging to the technical field of microbes. Background technique [0002] Plant fungal diseases have brought huge losses to agricultural production, and fruit postharvest fungal diseases are an important type of disease, resulting in a large reduction in fruit production, seriously affecting the edible value and market value of fruits, and resulting in the economic income of fruit farmers. reduce. In developed countries, the losses caused by postharvest diseases account for more than 15% of the total amount of fruits. In developing countries, due to the lack of necessary prevention and control technology and equipment, the losses caused by postharvest diseases account for 30% or even 30% of the total amount of fruits. higher up. The occurrence of postharvest fruit diseases has seriously affected modern agricultural production, especially the production and processing of high-end fruit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16A01N63/04A01P3/00C12R1/645
Inventor 全利红
Owner 北京莱万生物技术有限公司
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