Screening, identification and application of an antagonistic bacteria producing volatile antibacterial gas

A volatile and antagonistic technology, applied in applications, bacteria, microorganism-based methods, etc., can solve the problems of increased chemical residues in the dosage of chemical fungicides, increased threats to human health, and reduced disease prevention effects of drugs, and achieves high efficiency. The effect of prevention, high safety and simple operation

Active Publication Date: 2019-06-28
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main means of controlling postharvest diseases of fruits and vegetables is to use chemical fungicides. Although good control effects have been achieved, the long-term use of chemical fungicides has caused the bacteria to develop drug resistance, reducing the disease prevention effect of the agents, and eventually leading to chemical fungicides. The dosage of the agent and its chemical residues are greatly increased, and the threat to human health is also increased.

Method used

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  • Screening, identification and application of an antagonistic bacteria producing volatile antibacterial gas
  • Screening, identification and application of an antagonistic bacteria producing volatile antibacterial gas
  • Screening, identification and application of an antagonistic bacteria producing volatile antibacterial gas

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Example 1: Screening of antagonistic strain 12a

[0025] In the peach orchard without chemical fertilizers and pesticides, the rhizosphere soil of peach trees was collected, and the dilution separation method was used to make 10 -1 、10 -2 、10 -3 and 10 -4 Soil dilution solution, use a pipette gun to draw 10 -2 、10 -3 and 10 -4 Add 0.1mL of the soil dilution solution to the NA medium plate, spread it evenly with a sterile applicator, and mark the serial number. After standing for one hour, reverse the culture plate and cultivate it at 37°C. From the second day of culture, single bacterial colonies were selected according to the characteristics of the colony shape, and transplanted to NA medium respectively, and were used for subsequent experiments after 48 hours of culture. The purified strains were stored on NA slant medium at 4°C.

[0026] Antagonist bacteria were screened by plate buckle method. Inoculate the cake of brown rot fungus in the center of the PDA p...

Embodiment 2

[0028] Embodiment 2: application on the prevention and treatment of peach brown rot

[0029] After inoculating the preserved antagonistic bacteria 12a into 1000mL NB liquid medium for shaking and culturing for 48 hours, invert the bacterial solution (aseptic solution in the lower layer of the control) in the lower layer of the clapboard of the sealed tank, place Feicheng white peach on the upper layer, and seal the tank lid with vaseline. After fumigation for 20min, the fruit was taken out. In order to make the amount of bacterial infection uniform, a wound of 3 mm × 3 mm × 3 mm was made on each fruit thorn, and then 1 × 10 4 20 μL of cfu / mL spore suspension of brown rot fungus, store the above-mentioned treated fruits at 20±1°C, measure the diameter of the lesion every 24 hours, and calculate the antibacterial rate, see Table 1 for details. The experiment was repeated three times with 20 fruits per treatment.

[0030] Growth inhibition rate=(diameter of control lesion-diame...

Embodiment 3

[0031] Embodiment 3: application on the prevention and treatment of apple black spot

[0032] Inoculate the preserved antagonistic bacteria 12a into 1000mL NB liquid medium and shake it for 48 hours, then invert the bacteria solution on the lower layer of the partition of the sealed tank (as compared to the lower layer of sterile solution), place Marshal apples on the upper layer, seal the tank lid with Vaseline, and fumigate for 20 min Then take out the fruit. In order to make the amount of bacterial infection uniform, a wound of 3 mm × 3 mm × 3 mm was made on each fruit thorn, and then 1 × 10 4 20 μL of cfu / mL Alternaria apple spore suspension, the above-mentioned treated fruits were stored at 20±1°C, and the diameter of the lesion was measured every 24 hours, and the antibacterial rate was calculated. See Table 1 for details. The experiment was repeated three times with 20 fruits per treatment.

[0033] Growth inhibition rate=(diameter of control lesion-diameter of treate...

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Abstract

The invention provides an antagonistic strain 12a producing volatile antibacterial gas and its application in preventing and controlling fruit and vegetable diseases. The antagonistic strain 12a disclosed in the present invention is Bacillus vallismortis, and the preservation number is CGMCC No.12179. The antagonistic bacterial strain 12a of the present invention is obtained by collecting the rhizosphere soil of peach trees in Feicheng peach orchard without applying chemical fertilizers and pesticides, and the volatile antibacterial gas produced in its fermentation liquid has a strong antagonistic effect on peach brown rot (Monilinia fructicola) , while treating apple brown spot caused by Alternaria mali, sweet potato black spot caused by Ceratocystis fimbriata Ellis.Et Halsted, and citrus blue spot caused by Penicillium italicum Wehmer. Mildew and winter jujube black spot caused by Alternaria alternata also have obvious inhibitory effect, and can effectively prevent and control a variety of fruit and vegetable diseases. The biological control potential strains have broad market application prospects.

Description

(1) Technical field [0001] The invention relates to the screening, identification and application of an antagonistic bacteria in the field of microorganisms and biological control, in particular to the application of fumigation of bacterial liquid producing volatile antibacterial gas in the prevention and treatment of post-harvest diseases of fruits and vegetables. (2) Background technology [0002] Huge losses due to postharvest diseases of fruits and vegetables have become a global problem. At present, the main means of controlling postharvest diseases of fruits and vegetables is to use chemical fungicides. Although good control effects have been achieved, the long-term use of chemical fungicides has caused the bacteria to develop drug resistance, reducing the disease prevention effect of the agents, and eventually leading to chemical fungicides. The dosage of the agent and its chemical residue are greatly increased, and the threat to human health is also increased. There...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/02A23B7/144A23B7/155C12R1/07
CPCA23B7/144A23B7/155C12N1/02C12N1/205C12R2001/07
Inventor 石晶盈殷晓慧王庆国张畅
Owner SHANDONG AGRICULTURAL UNIVERSITY
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