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Preparation method and application of tupistra extract

A technology of open arrow and extract, which is applied in the field of preparation of open arrow extract, can solve the problems of poor antibacterial effect of the extract, high solvent toxicity, susceptibility to microorganisms, etc., and achieves broad antibacterial spectrum, high extraction rate and less residue Effect

Active Publication Date: 2019-05-17
INST OF CHEM IND OF FOREST PROD CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although physical fresh-keeping methods such as controlled atmosphere and refrigeration can effectively delay the physiological and biochemical processes of fruits and vegetables after harvest and maintain the freshness of fruits, their disadvantages are that once they leave the original physical environment, especially after mechanical damage, they are easily infected by microorganisms, especially fungi. mildew and rot
Although there are related reports on the use of the open arrow extract for antibacterial in the prior art, there are defects such as complex process, high solvent toxicity, large usage, high cost, large residue, and poor antibacterial effect of the extract.

Method used

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  • Preparation method and application of tupistra extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Open arrow rhizome coarse powder 18-30 mesh 100g, add 1000mL 95% ethanol (the volume fraction of ethanol is 95%), ultrasonic-assisted extraction at room temperature for 20min, soak at room temperature for 12h, stir well, then ultrasonicate at room temperature for 20min, then Placed in a water bath at 50°C for constant temperature extraction for 3h, suction filtration, and the same method was repeated twice; the filtrates were combined, concentrated under reduced pressure at 50°C to extract, freeze-dried to constant weight, and the yield was about 10%.

Embodiment 2

[0032] Open arrow rhizome coarse powder 18-30 mesh 200g, add 2000mL ethyl acetate, ultrasonic-assisted extraction at room temperature for 30min, then soak at room temperature for 18h, stir well, then ultrasonicate at room temperature for 20min, and then place it in a water bath at 55 ℃ constant temperature Extraction for 3 hours, suction filtration, and the same method was repeated twice; the filtrates were combined, concentrated under reduced pressure at 60°C to extract, freeze-dried to constant weight, and the yield was about 1.85%.

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Abstract

The invention discloses a preparation method and application of a tupistra extract. According to the preparation method of the tupistra extract, with tupistra rhizomes as raw materials and ethanol water or ethyl acetate as a solvent, extraction is conducted, then through concentration and drying, the tupistra extract is obtained, and when the solvent is ethanol water, the volume fraction of ethylalcohol is not smaller than 60%. According to the preparation method, ethanol water or ethyl acetate is used as the solvent for preparing the tupistra extract, one-step completion is achieved, multiple solvents do not need to be adopted for repeated extraction, the technical operation is simple, the extraction rate is high, the cost is low, and the method is environmentally friendly; through the prepared exact, growth of filamentous fungi can be effectively inhibited, particularly, the inhibition function on rotten fungi such as penicillium expansum, penicillium italicum wehmer, penicillium digitatum, aspergillus niger, rhizopus stolonifera and botrytis cinereal is obvious, the effect is good at low concentration (1mg / mL), the antimicrobial spectrum is wide, no harmful organic solvent residues exist, the method is environmentally friendly, the toxic or side effect on a non-target is small, and the method accords with an environment-friendly sustainable development idea.

Description

technical field [0001] The invention relates to a preparation method and application of an open arrow extract and belongs to the field of open arrow extraction. Background technique [0002] With the improvement of living standards, more and more people have begun to pay attention to the safety of preservatives in fruits and vegetables and other foods during storage. Traditional preservatives include benzimidazoles, such as carbendazim, tecdol, benlatx, Thiophantine, etc., as well as sec-butylamine and its derivatives, Xianbao, Baiduza, etc., are mostly chemically synthesized, and overdose drug use is common in some regions or crops, posing a risk to the quality and safety of agricultural products. [0003] Although physical preservation methods such as controlled atmosphere and refrigeration can effectively delay the physiological and biochemical processes of fruits and vegetables after harvesting and maintain the freshness of fruits, their disadvantages are: once they leav...

Claims

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Application Information

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IPC IPC(8): A61K36/896A61P31/10
Inventor 商士斌姚姝凤王丹刘鹤张海波高宏
Owner INST OF CHEM IND OF FOREST PROD CHINESE ACAD OF FORESTRY
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