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Topical Compositions Comprising Pichia Anomala and Chicory Root Extracts

A technology of Pichia anomaly and chicory root extracts, which can be applied in drug combinations, medical preparations containing active ingredients, drug delivery, etc., and can solve problems such as difficult penetration of exogenous hyaluronic acid

Active Publication Date: 2017-06-27
JOHNSON & JOHNSON CONSUMER COPANIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Furthermore, skin penetration of exogenous hyaluronic acid has proven difficult to achieve by topical application

Method used

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  • Topical Compositions Comprising Pichia Anomala and Chicory Root Extracts
  • Topical Compositions Comprising Pichia Anomala and Chicory Root Extracts
  • Topical Compositions Comprising Pichia Anomala and Chicory Root Extracts

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0135] The hyaluronic acid (HA) secretion assay described above was used to determine the production of hyaluronic acid in human dermal fibroblasts following treatment of cells with extracts from P. anterogenes and / or chicory root. Results are shown in Tables 1 and 2, where values ​​represent mean values ​​of independent studies.

[0136] The extract of Pichia anomalies is supplied in liquid form, processed as a 100% stock solution, and added directly to the culture medium in an appropriate amount until the final test concentration is reached.

[0137] Chicory root extract was a powder containing about 12% by weight glucosamine prepared as described in US Patent 8,652,532. Chicory root extract was resuspended in phosphate buffered saline (PBS) to make a 10% (w / v) stock solution. This stock solution was then further diluted in cell culture medium to reach the final test concentration.

[0138] The two extracts were added to the medium alone or together at the stated doses and...

Embodiment 2

[0147] To further investigate the effect of a wider range of concentrations, Pichia anomaly extracts from kiwifruit plants and chicory root extracts prepared as described in US Patent 8652532 were selected. Following the same protocol as in Example 1, the two extracts were added to the medium at the stated doses, either alone or together, and their effects on HA secretion were used to determine the fold change in HA production compared to the untreated case. The results are shown in Table 3. The fold change in Table 3 was used to determine the synergistic effect plus the additive effect when using each combination of the above formulations. Synergistic effects plus additive effects are reported in Table 4.

[0148] table 3

[0149]

[0150] Table 4

[0151]

[0152]

[0153] The results in Tables 3 and 4 clearly show that treatment of human dermal fibroblasts with the combination of Pichia anomaly and chicory root extracts resulted in an unexpected co-stimulati...

Embodiment 3

[0156] Combinations of the following ingredients were tested for HA production in the Hyaluronic Acid (HA) Secretion Assay: 1) Comprising Pichia Pastoris extract with resveratrol (formazan commercially available from Lonza Personal Care) Oxytetracycline), and 2) the chicory root extract described in Example 1.

[0157] The results are shown in Table 5.

[0158] table 5

[0159]

[0160]

[0161] The use of Pichia pastoris extract in combination with chicory root extract failed to synergistically produce HA in human dermal fibroblasts. This suggests that not all Pichia extracts are created equal.

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Abstract

The present invention provides a topical composition comprising an extract of Pichia anomala and an extract of chicory root that contains glucosamine.

Description

technical field [0001] The present invention provides a method of treating skin by topically applying to the skin a combination of Pichia anomala and chicory root extract. Furthermore, there is provided a topical composition comprising a combination of Pichia anomaly extract and chicory root extract. Background technique [0002] Human skin is composed of two parts, the superficial part (epidermis) and the deep part (dermis). Human skin undergoes certain aging processes. [0003] The epidermis is in contact with the external environment. The role of the epidermis is primarily to protect the body against dehydration and against external aggressions, whether chemical, mechanical, physical or infectious. The natural human epidermis is mainly composed of three types of cells: keratinocytes, melanocytes and Langerhans cells, of which keratinocytes account for the vast majority. The crucial role played by the skin of the body is due to the unique functions of each of these cel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9789A61K8/9728A61Q19/08A61Q19/00
CPCA61K8/60A61K8/97A61Q19/00A61Q19/08A61K8/99A61K36/062A61K36/28A61K8/9789A61Q19/007A61P17/00A61P39/00A61K2300/00A61K8/9728A61P17/18A61K8/345A61K9/0014A61K31/7008A61K36/064A61K8/73A61K2800/596A61Q17/00
Inventor A·T·巴列斯特罗斯K·马哈茂迪L·A·莫瑞拉R·帕萨M·D·索斯霍
Owner JOHNSON & JOHNSON CONSUMER COPANIES
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