958 results about "Aceglatone" patented technology

The invention relates to the technical field of microbial fermentation, and discloses a genetically engineered bacterium of high-yield D-pantothenic acid as well as a preparation method and application of the genetically engineered bacterium. The D-PA yield of the engineering bacteria is greatly improved through the following effects that acetolactate synthase IlvB and IlvN shunted by pyruvic acid are subjected to overexpression, meanwhile, a transcription attenuation region is deleted, and valine feedback inhibition sites are mutated; the method comprises the following steps: overexpressing keto-alcohol acid reductoisomerase IlvC and dihydroxy acid dehydratase IlvD, and converting the IlvC coenzyme preference from NADPH into NADH, so as to break through the upstream of D-PA synthesis; overexpression of PanB is carried out, overexpression of a serine-glycine transport system is carried out, and synthesis of 5, 10-methylenetetrahydrofolic acid is promoted; meanwhile, the expression of ketopantoic acid reductase PanE is enhanced; and finally, over-expressing pantothenic acid synthetase PanC, and increasing the content of a cofactor ATP required by the PanC. According to the engineering bacteria provided by the invention, through overall reinforcement of the multiple modules, cell growth is improved due to balance of cofactors and increase of D-PA, and the yield of D-PA reaches an unprecedented height.

The invention provides a composition for improving bone joint health as well as a preparation method and application thereof, and relates to the technical field of bone joint health products. The composition for improving bone joint health is prepared from the following components in parts by mass: 0.5 to 5 parts of collagen peptide, 5 to 30 parts of mangosteen extract, 0.5 to 3 parts of non-denatured II type collagen peptide, 0.1 to 2 parts of milk mineral salt, 0.3 to 3 parts of bone collagen peptide, 0.1 to 2 parts of cartilage extract, 0.1 to 2 parts of N-acetylglucosamine, 0.1 to 2 parts of sodium hyaluronate and 0.3 to 3 parts of turmeric extract. The composition obtained by repeatedly compounding the mangosteen extract, the turmeric extract, the cartilage extract and the sodium hyaluronate has the advantages of low cost and high safety, and has a better effect on preventing or improving osteoarthrosis.

The invention discloses a preparation method and application of a zinc-manganese bimetal organic framework derived composite nano-enzyme, and belongs to the technical field of nano-materials. Zinc-manganese bimetal organic framework nanoparticles prepared by reacting ZIF-8 with manganese (III) acetylacetonate are used as a template, and are calcined at different temperatures in a tubular furnace to obtain zinc-manganese bimetal organic framework derivative composite nano-enzymes with different morphologies. The nano enzyme has peroxidase-like activity, glutathione peroxidase-like activity and catalase-like activity, and has a wide application prospect in anti-tumor treatment.

The invention discloses N-acetylglucosamine transferase AcetRL-R349H and an application thereof, and belongs to the technical field of functional enzymes. The amino acid sequence of the N-acetylglucosamine transferase AcetRL-R349H is as shown in SEQ ID NO. 5 (sequence identifier number 5). The invention further discloses the application of the N-acetylglucosamine transferase AcetRL-R349H in the preparation of the chitosan oligosaccharide. The N-acetylglucosamine transferase AcetRL-R349H disclosed by the invention is obtained by carrying out mutation modification on the N-acetylglucosamine transferase AcetRL, and can be used for producing the chitosan oligosaccharide with the polymerization degree of 4 to 6, especially (GlcNAc) 6. The product spectrum of the N-acetylglucosamine transferase is widened, and the N-acetylglucosamine transferase can be used for preparing high-polymerization-degree chitosan oligosaccharide and has a wide application prospect.

The invention discloses an N-acetylglucosamine transferase AcetRS-R346D and an application thereof, and belongs to the technical field of functional enzymes. And the amino acid sequence of the N-acetylglucosamine transferase AcetRS-R346D is as shown in SEQ ID NO. 3. The invention further discloses the application of the N-acetylglucosamine transferase AcetRS-R346D in the preparation of the chitosan oligosaccharide. The N-acetylglucosamine transferase AcetRS-R346D disclosed by the invention is obtained by carrying out mutation modification on the N-acetylglucosamine transferase AcetRS, and can be used for producing the chitosan oligosaccharide with the polymerization degree of 4 to 7, especially (GlcNAc) 7. The product spectrum of the N-acetylglucosamine transferase is widened, and the N-acetylglucosamine transferase can be used for preparing high-polymerization-degree chitosan oligosaccharide and has a wide application prospect.

The invention belongs to the technical field of chondroitin sulfate biosynthesis, and discloses a recombinant strain for high-yield chondroitin sulfate, application of the recombinant strain and a preparation method of the chondroitin sulfate. The recombinant strain provided by the invention can be used for simultaneously expressing UDP-glucosamine isomerase, UDP-glucose dehydrogenase, chondroitin polymerase, glucosamine synthetase, phosphoglucosamine mutase, UDP-N-acetylglucosamine pyrophosphorylase, glucose-6-phosphate isomerase and glucose phosphate mutase; according to the present invention, glucose-1-phosphate uridyl transferase, ATP sulfonase, sulfotransferase, pyruvate kinase and APS kinase are adopted, and the enzyme molecules are matched with the original carbon metabolism pathway in the recombinant strain cell to jointly form the enzyme-linked pathway having the efficient CSA synthesis ability, such that the high-yield and high-purity preparation of the CSA can be achieved; the method has the advantages of simple operation, high biological safety of the obtained product, easy realization of large-scale production and the like, and has a good application prospect.

The invention relates to the technical field of dye synthesis, and particularly discloses a genetically engineered bacterium for synthesizing N-acetyl blue as well as a construction method and application of the genetically engineered bacterium. The supply capacity of precursor substances glutamic acid and glutamine of the genetically engineered bacterium is high, the fermentation process is simple, the production intensity of the precursor substances glutamine and N-acetylglutamine is improved by cutting off a consumption path, relieving negative feedback inhibition and enhancing the expression level of key genes through the genetically engineered bacterium, and finally the yield of N-acetylblue reaches 41.2 g / L.

The invention discloses a culture medium for culturing a liver cancer micro-tumor model and a culture method thereof. The invention relates to the technical field of biology, and provides a culture medium for culturing a liver cancer micro-tumor model, and the culture medium is composed of an antibacterial and antifungal agent tri-antibody, HEPES, GlutaMax, a human recombinant protein EGF, FGF-10, HGF, IL-2, IL-15, R-spondin 1, calomel, gastrin, A83-01, N-acetyl-L-cysteine, nicotine, an N2 additive, B27 and a basal culture medium. The liver cancer micro-tumor model obtained by the method can accurately reflect various characteristics of the original focus of a patient, and is a good scientific research experiment model and a preclinical experiment model in the field of precise tumor diagnosis and treatment. It can be foreseen that the culture method has wide application prospects in the fields of liver cancer research and clinical diagnosis and treatment.

The invention relates to the technical field of cosmetics, and particularly discloses a composition with after-sun repairing and whitening effects and a preparation method thereof. According to the present invention, various efficient components such as the ergothioneine compound, the acetylchitosamine, the nicotinamide, the sodium hyaluronate, the inositol, the aloe vera extract, the glycerophosphoinositide choline salt, the tetrahydromethylpyrimidine carboxylic acid, the oat beta-glucan compound, the citrus peel extract compound and the skin brightening agent are scientifically matched; according to the present invention, the excellent after-sun repairing and whitening effect is achieved, the comprehensive after-sun skin care is achieved, the skin state can be improved, and the skin health degree can be improved. The application creatively finds that when the glycerophosphoinositide choline salt, the citrus peel extract compound and the skin brightening agent are compounded, the effects of the composition in the aspects of after-sun repairing and whitening can be improved at the same time, and the comprehensive effect is relatively good.

The invention discloses corynebacterium glutamicum for producing N-acetylglucosamine and application of the corynebacterium glutamicum, and belongs to the technical field of synthetic biology. Specifically, the corynebacterium glutamicum overexpresses a GNA1 gene from a brewer's yeast S288C controlled by a tacM promoter, a glmS gene from corynebacterium glutamicum S9114 and a yqab gene from escherichia coli K12 MG1655, and simultaneously has five gene defect types of nagA, nagB, ldhA, msccg and msccg2 in a genome and two gene single base mutation types of odhA and glnA, and the codon of the glnA for coding the 405th amino acid is mutated from TAC to TTC. By blocking excretion of glutamic acid of corynebacterium glutamicum and enhancing conversion of glutamic acid to glutamine, the supply capacity of a precursor of corynebacterium glutamicum is enhanced, and an optimal way for synthesizing N-acetylglucosamine is obtained, so that efficient production of N-acetylglucosamine in corynebacterium glutamicum is realized, and the yield of N-acetylglucosamine in corynebacterium glutamicum is increased. The method has important economic value and social significance.

The invention relates to the technical field of genetic engineering, and discloses a genetically engineered bacterium for producing L-arginine as well as a construction method and application of the genetically engineered bacterium. Escherichia coli is taken as a starting strain, and the following treatment is performed: overexpressing genes gdhA and gdh of glutamate dehydrogenase, promoting generation of glutamic acid, overexpressing and coding arginine transporter gene argO to enhance extracellular transport of arginine, introducing exogenous argJ to replace a linear synthesis pathway in large intestines, improving the utilization rate of acetyl coenzyme A and accelerating the synthesis efficiency of arginine; a glutamine synthetase gene glnA is over-expressed, and glutamine synthesis is enhanced; the pckA is overexpressed to promote ATP synthesis; meanwhile, speA and adiA genes for coding the arginine decarboxylase are deleted, so that further synthesis of putrescine from L-arginine is inhibited, and the L-arginine accumulation capability of the strain is improved. Through the combined action of the treatment, a genetically engineered bacterium with extremely high L-arginine production yield is finally obtained, and the shake flask fermentation yield can reach 13.7 g / L.

The invention relates to the technical field of synthetic biology, metabolic engineering and cell culture. The present invention provides a cell for the production of a negatively charged, preferably sialylated, oligosaccharide wherein the cell is genetically engineered to have or express, preferably overexpress, a hydrolytic UDP-N-acetyl-D-glucosamine-2-epimerase. The invention further provides the use of said cells in culture or incubation. Also described are methods of producing negatively charged, preferably sialylated oligosaccharides using the cells, and purification of the negatively charged, preferably sialylated oligosaccharides.

The present invention relates to a recombinant yeast cell that produces UDP-D-glucuronic acid and / or UDP-N-acetyl-D-glucosamine compared to a wild type cell of the recombinant yeast cell; and optionally, the yield of 3 '-phosphate-adenylate sulfuric acid is increased. The invention also relates to a method for producing heparan sulfate, which comprises a step of culturing the recombinant yeast cell, further relates to an application of the non-invasive recombinant yeast cell for producing heparan sulfate, and finally relates to heparan sulfate obtained by applying the method.

The invention belongs to the technical field of gene engineering, and particularly relates to an LgtA mutant and application thereof in production of human milk oligosaccharide. According to the present invention, a gene editing technology is adopted to mutate a key enzyme beta-1, 3-N-acetyl glucosamine transferase for synthesizing an intermediate product LNT II so as to obtain a mutant having at least one of R13H mutation, L24M mutation and R205C mutation on the basis of a wild type LgtA represented by SEQ ID NO.1; further, the mutant is applied to the construction process of production plasmids of the LNTII, the LNnT and the LNT, and the yield of the LNTII, the LNnT and the LNT is further increased.

The present application relates to the technical field of biocatalysis and biosynthesis, and specifically discloses a natural blue pigment and a biosynthesis method therefor. The present application uses metabolically engineered bacteria to express indigoidine synthetase and phosphopantetheinyl transferase, to catalyze glutamine and N-acetylglutamine to biosynthesize a natural blue pigment N-acetylindigoidine, and deduces its molecular structure by means of mass spectrometry, nuclear magnetic resonance and other methods. The present application achieves catalytic synthesis of N-acetylindigoidine from glutamine and N-acetylglutamine in Escherichia coli, Corynebacterium glutamicum, Saccharomyces cerevisiae and Streptomyces. Compared with indigoidine, N-acetylindigoidine has a maximum absorption wavelength of 584 nm, possesses better color brightness, and has more stable coloration that is not easily faded, with a very broad application range and industrial production application prospects.

The invention provides a synthesis method of (S)-3-aminobutyronitrile, which specifically comprises the following steps: (1) addition of diketene and ammonia to prepare acetoacetamide; (2) performing an enzyme catalytic reaction on the acetoacetamide obtained in the step (1) in the presence of transaminase, and purifying after the reaction is completed to obtain (S)-3-aminobutanamide; and (3) dehydrating the (S)-3-aminobutanamide obtained in the step (2) to obtain the (S)-3-aminobutyronitrile. According to the synthesis method, the synthesis route is simplified, the production efficiency of the (S)-3-aminobutyronitrile is improved, industrial three wastes are reduced, the use of highly toxic substances is avoided, the cost reduction and benefit improvement in the industrial amplified production are facilitated, and the whole route embodies the value of green chemical synthesis.

The invention relates to the technical field of cosmetics, and particularly discloses an oxyresveratrol-containing multi-vitamin anti-pollution composition suitable for sensitive skin and application of the oxyresveratrol-containing multi-vitamin anti-pollution composition, the multi-vitamin anti-pollution composition comprises oxyresveratrol, epigallocatechin gallic acid glucoside, acetylchitosamine and annona squamosa extract; the mass ratio of the oxyresveratrol to the epigallocatechin gallic acid glucoside to the acetylchitosamine to the annona squamosa extract is (0.1 to 3) to (0.001 to 0.1) to (1 to 10) to (0.1 to 5). The anti-pollution composition provided by the invention achieves the effects of relieving, repairing and whitening by simultaneously resisting various pollutants such as air pollution, ultraviolet rays, heavy metals and the like, and comprehensively solves the problems of yellow smell and sensitivity of skin.

The present application relates to the technical field of biocatalysis and biosynthesis, and specifically discloses a blue pigment and a biosynthesis method thereof. In the present application, an indigoidine synthetase and a 4′-phosphopantetheinyl transferase are expressed by a metabolically engineered strain to catalyze the biosynthesis of the blue pigment N-acetyl-indigoidine from glutamine and N-acetylglutamine, and a molecular structure of the blue pigment is inferred by mass spectrometry, nuclear magnetic resonance spectroscopy, etc. The present application achieves the catalytic synthesis of N-acetyl-indigoidine from glutamine and N-acetylglutamine in Escherichia coli (E. coli), Corynebacterium glutamicum (C. glutamicum), Saccharomyces cerevisiae (S. cerevisiae), and Streptomyces. Compared with indigoidine, N-acetyl-indigoidine has a maximum absorption wavelength of 584 nm, and a stable color having high brightness that is not easy to fade. Thus, the blue pigment shows an extensive application range and a promising industrial production prospect.

The invention discloses a composition for improving the stability of oleuropein in a solution as well as a preparation method and application of the composition. The composition comprises oleuropein and at least one stabilizer, and the stabilizer is selected from the group consisting of anisic acid, lactobionic acid, glycyrrhizic acid, 3, 3-thiodipropionic acid, N-acetyl-L-glutamine, propyl-cysteine and lauramidopropyl betaine; and the pH value of the aqueous solution of the composition is 4.0-8.0. The preparation method comprises the following steps: dissolving oleuropein in water, adding the stabilizer, and uniformly mixing. Through simple physical mixing, by utilizing the synergistic effect of the specific stabilizer and the oleuropein, the degradation and discoloration of the oleuropein in the storage process are remarkably inhibited. Experiments show that after the preferable composition is preserved for 30 days in an open manner, the retention rate of the oleuropein still reaches up to 80% or above, and is far superior to that of blank control and a traditional liposome wrapping method. The composition is simple and convenient in preparation process and low in cost, and provides reliable technical support for wide application of oleuropein in liquid products such as food, medicine and cosmetics.

The invention relates to the field of genetic engineering, in particular to a genetically engineered bacterium for efficiently synthesizing 5-aminolevulinic acid. According to the invention, a synthetic biological technology and a genetic engineering means are utilized, escherichia coli E.coli BL21 (DE3) is taken as an original strain, a C5 way of the escherichia coli E.coli BL21 (DE3) is modified, and degradation of glutamic acid is reduced by knocking out a gadA gene and replacing a promoter of an argE gene; the synthesis of the 5-aminolevulinic acid is increased by enhancing the expression of gdhA, hemAS and hemL genes, replacing promoters and increasing the carbon flux flowing to the 5-aminolevulinic acid; in addition, by knocking out acetic acid synthesis genes ackA, poxB and pta, the loss of carbon flux flowing to TCA circulation is reduced, so that the purpose of increasing the yield of 5-aminolevulinic acid is achieved.

The invention relates to the technical field of microbial metabolism engineering, in particular to a corynebacterium glutamicum producing strain for efficiently producing D-pantothenic acid as well as a construction method and application of the corynebacterium glutamicum producing strain. The construction method specifically comprises the following steps: (1) by taking wild corynebacterium glutamicum ATCC 13032 as a chassis, weakening ilvA, and constructing DPA1 through mutation expression; (2) knocking out the quinone pyruvate oxidoreductase and inserting a panBCE gene expression cassette into the quinone pyruvate oxidoreductase to construct DPA2; (3) introducing and using a pEC-xk99E overexpression plasmid to screen and express Escherichia coli W3110, Bacillus subtilis 168, and endogenous acetohydroxy acid synthase, acetolactate isomerized reductoisomerase and 2-hydroxy acid dehydratase, and carrying out a step (2) of carrying out a step (3) of screening and expressing the Escherichia coli W3110, Bacillus subtilis 168, and endogenous acetohydroxy acid synthase, acetolactate isomerized reductoisomerase and 2-hydroxy acid dehydratase in the DPA2; and (4) under the control of a strong promoter, carrying out tandem overexpression on the screened ilvBNCD gene by using pEC-xk99E, constructing DPA3, and enhancing a pantothenic acid main synthesis pathway and an upstream pathway pulling force, thereby obtaining the corynebacterium glutamicum for efficiently producing D-pantothenic acid. The strain provided by the invention can produce D-pantothenic acid with high yield and high conversion rate by utilizing a carbon source, and has important industrial application value.

The invention belongs to the technical field of synthetic biology, and particularly relates to recombinant escherichia coli for efficiently producing O-acetyl-L-homoserine as well as a construction method and application of the recombinant escherichia coli. According to the invention, CRISPR-Cas9 gene editing is combined with metabolic modification, so as to precisely integrate OAH biosynthesis key nodes: introducing and overexpressing a mutant metX gene at a genome pseudogene site to enhance the expression of homoserine acetyltransferase, knocking out byproducts such as poxB and mgsA to synthesize genes, and carrying out high-yield synthesis of OAH. Corynebacterium glutamicum thrA (anti-feedback inhibition), aspB and bacillus subtilis acsA-acuA genes (construction of an acetic acid switch) are introduced, gene expression of gltA, tpiA and the like is optimized to increase supply of precursors such as acetyl CoA and the like, and finally a stable strain without plasmids, antibiotics and inducers is obtained. The production cost is effectively reduced, and the method has the advantage of high genetic stability and shows a good application prospect in OAH industrial production.

The present invention relates to antibody conjugates which are particularly suitable for targeting CEA-expressing cells, in particular tumor cells. An antibody conjugate according to the present invention has the structure (1): AB-[(L6) b-{Z-L-D} x] y (1) wherein AB is an antibody capable of targeting a tumor expressing CEA; l is a joint connecting Z to D; z is a linking group; l6 is-GlcNAc (Fuc) w-(G) j-S-(L7) w-wherein G is a monosaccharide, j is an integer in the range of 0 to 10, S is a sugar or a sugar derivative, GlcNAc is N-acetylglucosamine, and Fuc is fucose, w is 0 or 1, w'is 0, 1 or 2, and L7 is-N (H) C (O) CH2-,-N (H) C (O) CF2-or-CH2-; d is selected from the group consisting of an anthracycline, a camptothecin, a tubulysin, an endiyne, an amanitine, a duocarmycin, a maytansinoid, an aurestatin, eribulin, a BCL-XL inhibitor, a miscanthus hemiphyllum, a KSP inhibitor, a TLR agonist, an indolo-benzodiazepine # imgabs 0 # dimer or a pyrrolo-benzodiazepine # imgabs 1 # dimer (PBD), and analogs or prodrugs thereof; b is 0 or 1; x is 1 or 2; and y is 1, 2, 3 or 4. The invention further relates to a method for preparing said antibody conjugate of structure (1) and to the use of said antibody conjugate of structure (1).

The invention relates to the technical field of optical adhesion agents, and discloses a water-resistant polyvinyl alcohol optical adhesion agent and a preparation method and application thereof, and the water-resistant polyvinyl alcohol optical adhesion agent comprises the following components in parts by weight: 88-95 parts of deionized water, 4-7 parts of polyvinyl alcohol, 0.1-2 parts of lithium magnesium stone powder, 0.1-2 parts of glutaraldehyde, 0.1-0.5 part of metal salt and 0.1-2 parts of polyethyleneimine. The water-resistant polyvinyl alcohol optical adhesive has the advantages that the water resistance of a polarizing plate can be effectively enhanced by the prepared water-resistant polyvinyl alcohol optical adhesive due to the laminated assembly trend and the strong hydrogen-bond interaction of the lithium magnesium stone powder and binary, ternary and multi-element chemical bonds formed by glutaraldehyde, polyethyleneimine and metal salt with acetoacetyl functional groups in the polyvinyl alcohol respectively.

The invention discloses a psychrophilic salt activated beta-N-acetylglucosaminidase mutant D180S and application thereof, and relates to the technical field of gene engineering. The amino acid sequence of the mutant D180S is shown as SEQ ID NO.1, the optimum pH value of the mutant D180S is 6.0, the optimum temperature of the mutant D180S is 5 DEG C, and compared with recombinant wild beta-N-acetylglucosaminidase with the amino acid sequence shown as SEQ ID NO.5, the optimum temperature of the mutant D180S is reduced by 40 DEG C. The enzyme activity of the recombinant wild beta-N-acetylglucosaminidase opt-HJ5NagNC is reduced along with the increase of the salt concentration in a reaction system, while NaCl, NaBr, NaI, NaF and NaNO3 can activate the activity of D180S to different extents, and the mutant can be applied to the field of biotechnology under the low-temperature salt-containing environment requirement, and especially can be applied to the industries of food, brewing, washing, papermaking, sewage treatment and the like.

The mixture as the micronutrient composition is a combination of alpha-ketoglutaric acid, nicotinamide or nicotinic acid, L-proline, L-glutamine, L-citrulline, sodium pyruvate, pantothenic acid, L-cysteine or N-acetylcysteine, magnesium malate, magnesium citrate, and methionine. The mixture is effective for the treatment of diseases due to oxidative stress. More specifically, the mixtures and cores as micronutrient compositions are effective in reversing saccharification in diabetes mellitus.

The invention relates to the technical field of aquaculture, in particular to a leiocassis longirostris intestinal health functional additive based on spermaceti soxhlet metabolite. The Leiocassis longirostris intestinal health functional additive comprises the following functional components: chondroitin sulfate, N-acetyl-D-galactosamine, 4 ', 4', 4 ', 4', 4 ', 4', 4 ', 4', 4 ', 4', 4 ', 4'- The composition is prepared from the following raw materials: 4, 6-dihydroxyflavone, polydatin, ascorbic acid, glucoside, arabinogalactan, 3-O-beta-D-galactopyranosyl-L-arabinose, tyramine, 4A-hydroxytetrahydrobiopterin, hyaluronic acid and 3-mercapto-2-butanol. According to the technical scheme, the technical problem that an existing probiotic viable bacterium preparation is difficult to meet the intestinal health management requirement in intensive culture of the leiocassis longirostris can be solved. Compared with the prior art, the scheme is more efficient, stable and safe, healthy development of the Leiocassis longirostris breeding industry can be effectively promoted, and the method has ideal application and popularization prospects.

The present invention provides a chemical synthesis method of EPS-G7, wherein β-cyclodextrin A is acetylated to obtain β-acetylcyclodextrin B, B is ring-opened in a mixed solution of acetic anhydride / concentrated sulfuric acid to obtain a chain acetylated heptose C, p-nitrophenol is condensed with intermediate C using boron trifluoride acetonitrile to obtain a key intermediate p-nitrophenyl (G1)-O-acetyl-D-maltoseptaglycoside D with α as the dominant configuration, D is removed from the acetyl group under the action of sodium methoxide to obtain pNP-G7 (p-nitrophenyl (G1)-D-maltoseptaglycoside), pNP-G7 is in the presence of p-toluenesulfonic acid and dimethoxyethane, and acetal protection is formed between the hydroxyl groups on C4 and C6 of the terminal monosaccharide to obtain EPS-G7. The reaction raw materials used in the method of the present invention are cheap and readily available, and the chemical synthesis of α-amylase detection reagent EPS-G7 is realized, which is a simple, economical and efficient synthesis method.

The invention discloses a composition with sensitive skin repairing and anti-aging functions as well as an emulsion and application of the composition, and belongs to the technical field of cosmetics. The composition specifically comprises a pseudoalteromonas fermentation product extract, collagen, palmitoyl tripeptide-5, acetylchitosamine, sodium hyaluronate, silanetriol, caffeine and acetyl hexapeptide-8, and can promote the generation of laminin-5 and integrin beta1, promote the vitality of keratinocytes and fibroblasts, inhibit the growth of the keratinocytes and the fibroblasts, inhibit the growth of the keratinocytes and the fibroblasts, inhibit the growth of the keratinocytes and the fibroblasts, and promote the growth of the keratinocytes and the fibroblasts. The generation of inflammatory factors (IL-6) is inhibited; the generation of collagen IV and collagen VII is promoted; the invention further provides an emulsion prepared from the composition, and the optimal formula of the emulsion is obtained through optimization. The active composition disclosed by the invention has a wide application prospect in the field of cosmetics, and particularly has important significance in sensitive skin repair and anti-aging directions.

The invention discloses a gymnadenia conopsea octasaccharide-decaose mixture as well as a preparation method and application thereof, and belongs to the technical field of medicines. The monosaccharide of the octasaccharide-decataccharide mixture of the gymnadenia conopsea comprises N-acetamino galactose, fucose, glucuronic acid or sugar alcohol thereof, galactose, N-acetylglucosamine, dehydrated talose and a sulfate group. The octasaccharide to decataccharide mixture of the gymnadenia conopsea disclosed by the invention can be used for remarkably prolonging the time of activating partial thromboplastin and prothrombin and effectively inhibiting endogenous and common coagulation pathways. The octasaccharide-decaose mixture of the gymnadenia conopsea can be used as a potential endogenous factor X enzyme inhibitor for preparing medicines for treating thrombotic cardiovascular diseases.