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Pichia anomala strain capable of directly preparing xylose into alcohol

A kind of abnormal Pichia pastoris, Pichia pastoris technology, applied in the direction of microorganism-based methods, microorganisms, fungi, etc., to achieve the effect of broadening the source of raw materials, reducing production costs, and reducing costs

Inactive Publication Date: 2011-08-17
YUNNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to overcome the deficiency that the current alcohol producing strains—Saccharomycescerevisiae (non-engineering bacteria) cannot directly use xylose to ferment alcohol, and the ability of xylose engineering strains to utilize xylose can not meet the needs of industrialization, and provide a strain Pichia anomaly strains that can directly use xylose to produce alcohol, thereby expanding the source of raw materials for the production of ethanol by the current ethanol production strains, and reducing the cost of ethanol production from cellulose

Method used

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  • Pichia anomala strain capable of directly preparing xylose into alcohol
  • Pichia anomala strain capable of directly preparing xylose into alcohol

Examples

Experimental program
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Effect test

Embodiment 1

[0021] First, the cellulose raw material is treated to become xylose liquid as a carbon source, added to the conventional medium for yeast alcohol fermentation, adjusted to pH 4.0, and started to use.

[0022] Secondly, alcohol fermentation: the MLB1 bacterial strain is cultivated according to the conventional liquid seed culture method of microbial fermentation, with sterilized xylose liquid medium (sugar concentration is 2%), after a series of culture in shake flasks and seed tanks, when each milliliter When the number of yeasts in the culture solution is greater than 1×108, put 1 / 15 of the fermenter culture solution (sterilized, sugar concentration 5%) into the fermenter for cultivation, the temperature is controlled at 28°C, and the pH value Control at 4.0, maintain continuous or intermittent ventilation of sterile air, that is, ventilation for 10 minutes every 1 hour. Incubate for 48 hours. 100% of xylose is utilized to produce alcohol with a yield greater than 50%.

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Embodiment 2

[0025] The difference from Example 1: the pH value of the culture solution is 4.5, the sugar concentration of the seed culture solution is 3%, the sugar concentration of the fermenter culture solution is 7%, and the cultivation time is 60 hours.

Embodiment 3

[0027] The difference from Example 1: the pH value of the culture solution is 5.2, the sugar concentration of the seed culture solution is 5%, the sugar concentration of the fermenter culture solution is 10%, and the cultivation time is 72 hours.

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Abstract

The invention relates to a Pichia anomala strain capable of directly utilizing xylose, belonging to the technical field of industrial microbial fermentation engineering. The strain MLB1 provided by the invention is separated out from the natural ecological environment in the suburb of Yuanjiang County, Yunnan Province, and is a wild-type strain capable of directly fermenting xylose into alcohol. Taxonomic identification shows that the strain belongs to Pichia anomala, the strain is preserved in China General Microbiological Culture Collection Center, and the preservation number is CGMCC NO.4252. The strain can directly ferment xylose or glucose into alcohol 100%, the alcohol yield based on the xylose carbon source is higher than 50%, and the alcohol conversion rate based on the glucose carbon source is higher than 60%. The Pichia anomala strain capable of directly preparing xylose into alcohol makes up the deficiency of the xylose utilization capability of the xylose engineering strain, thereby widening the source range of alcohol-producing materials of the existing alcohol-producing strain, and lowering the cellulose alcohol-producing cost. Simultaneously, the invention also provides a genetic basis for microzyme-based alcohol fermentation from xylose.

Description

Technical field: [0001] The invention relates to a Pichia anomaly strain that can directly utilize xylose to produce alcohol, and belongs to the technical field of industrial microbial fermentation engineering. Background technique: [0002] Environment, energy and sustainable development have become global issues, among which energy is an important material basis of human society. With the rapid development of modern industry, agriculture and national defense science and technology, the consumption of energy has increased significantly. A comprehensive energy crisis has made human beings look for alternative energy sources. The proportion of renewable biomass resources must be gradually expanded. Ethanol has the following advantages as an alternative fuel: (1) The production cost is relatively low; (2) The pollution to the environment is much smaller than that of gasoline and diesel; (3) 20% ethanol and gasoline are mixed, and the car engine does not need to be modified ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12R1/84
Inventor 乔敏贾东晨余泽芬张克勤周薇
Owner YUNNAN UNIV
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