Pichia anomala strain capable of directly preparing xylose into alcohol
A kind of abnormal Pichia pastoris, Pichia pastoris technology, applied in the direction of microorganism-based methods, microorganisms, fungi, etc., to achieve the effect of broadening the source of raw materials, reducing production costs, and reducing costs
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Embodiment 1
[0021] First, the cellulose raw material is treated to become xylose liquid as a carbon source, added to the conventional medium for yeast alcohol fermentation, adjusted to pH 4.0, and started to use.
[0022] Secondly, alcohol fermentation: the MLB1 bacterial strain is cultivated according to the conventional liquid seed culture method of microbial fermentation, with sterilized xylose liquid medium (sugar concentration is 2%), after a series of culture in shake flasks and seed tanks, when each milliliter When the number of yeasts in the culture solution is greater than 1×108, put 1 / 15 of the fermenter culture solution (sterilized, sugar concentration 5%) into the fermenter for cultivation, the temperature is controlled at 28°C, and the pH value Control at 4.0, maintain continuous or intermittent ventilation of sterile air, that is, ventilation for 10 minutes every 1 hour. Incubate for 48 hours. 100% of xylose is utilized to produce alcohol with a yield greater than 50%.
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Embodiment 2
[0025] The difference from Example 1: the pH value of the culture solution is 4.5, the sugar concentration of the seed culture solution is 3%, the sugar concentration of the fermenter culture solution is 7%, and the cultivation time is 60 hours.
Embodiment 3
[0027] The difference from Example 1: the pH value of the culture solution is 5.2, the sugar concentration of the seed culture solution is 5%, the sugar concentration of the fermenter culture solution is 10%, and the cultivation time is 72 hours.
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