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Method for producing bioethanol through coffee waste

A bioethanol, waste technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as fossil fuel pollution

Active Publication Date: 2019-08-09
德宏丁香咖啡有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The massive consumption of fossil fuels has caused serious pollution

Method used

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  • Method for producing bioethanol through coffee waste
  • Method for producing bioethanol through coffee waste

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Experimental program
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Effect test

Embodiment 1

[0025] The composition of coffee waste was analyzed, and the results are shown in Table 1.

[0026] Table 1 Composition of coffee waste materials

[0027]

[0028] The coffee peel may contain coffee peel or coffee peel + pectin + shell.

Embodiment 2

[0030] A. Acid hydrolysis: add sulfuric acid in deionized water, the concentration of sulfuric acid is 0.4-0.6 M, then add coffee waste, the ratio of coffee waste to water is (kg / L)=1:3-1:5, temperature Stir for 1 hour at 100°C;

[0031] B. Enzymolysis: lower the coffee waste acid hydrolysis solution to room temperature, adjust the pH value to 4-5, control the temperature at 30-37°C, add yeast, and ferment for 48-96 hours to obtain coffee waste enzymatic hydrolysis solution ;

[0032] C. Distillation: Distill the coffee waste enzymatic hydrolysis solution at 75-85°C and cool to obtain bioethanol.

[0033] The source of Pichia anomaly is the Pichia anomaly that degrades the coffee rind efficiently and is re-isolated from commercial strains domesticated by the China Coffee Engineering Research Center in the fermentation of coffee rinds. Pichia anomala CERCC No. 3.

[0034] According to this method, experiment is carried out, and each parameter and ethanol yield are shown in ...

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Abstract

The invention provides a method for preparing bioethanol from coffee waste (coffee grounds, shells and peel). The method includes the steps of adding the coffee grounds and sulfuric acid to deionizedwater for hydrolysis for 1 hour at 100 DEG C, adding abnormal pichia pastoris at 30-37 DEG C for fermentation for 48-96 hours to obtain coffee waste enzymatic hydrolysate, conducting boiling reactionat 78.5 DEG C, and conducting cooling to obtain the bioethanol. The coffee waste obtained after coffee beans are used is recycled, the bioethanol is prepared through thermal reaction, considerable economic benefits can be brought, the bioethanol is friendly to the environment, and the environmental pollution is reduced. The bioethanol prepared through the method can be used for partly replacing automotive fuel.

Description

technical field [0001] The invention belongs to the technical field of biochemistry, and relates to a method for utilizing food waste residues, in particular to a method for producing ethanol by using coffee residues. Background technique [0002] During the harvesting of coffee cherries, different coffee wastes are produced due to different processing methods: wet-processed coffee produces a large amount of coffee husks (accounting for 20%), while dry processing (sun-drying) produces a large amount of coffee husks, fruit Gums and husks (45% in total). Coffee beans are ground by a coffee machine and brewed to become a coffee drink, but there is also left an extraction residue that accounts for 70-80% of the weight of coffee beans. In the production of instant coffee, manufacturers increase the grinding capacity of the coffee machine and try to grind the coffee beans smaller, leaving 50-65% of the coffee grounds after extraction. At present, the utilization of coffee waste ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/10C12R1/84
CPCC12P7/10Y02E50/10
Inventor 陈振佳梁静思张枝润郑志锋闫培睿黄少栋
Owner 德宏丁香咖啡有限公司
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