Method for producing bioethanol through coffee waste
A bioethanol, waste technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as fossil fuel pollution
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Embodiment 1
[0025] The composition of coffee waste was analyzed, and the results are shown in Table 1.
[0026] Table 1 Composition of coffee waste materials
[0027]
[0028] The coffee peel may contain coffee peel or coffee peel + pectin + shell.
Embodiment 2
[0030] A. Acid hydrolysis: add sulfuric acid in deionized water, the concentration of sulfuric acid is 0.4-0.6 M, then add coffee waste, the ratio of coffee waste to water is (kg / L)=1:3-1:5, temperature Stir for 1 hour at 100°C;
[0031] B. Enzymolysis: lower the coffee waste acid hydrolysis solution to room temperature, adjust the pH value to 4-5, control the temperature at 30-37°C, add yeast, and ferment for 48-96 hours to obtain coffee waste enzymatic hydrolysis solution ;
[0032] C. Distillation: Distill the coffee waste enzymatic hydrolysis solution at 75-85°C and cool to obtain bioethanol.
[0033] The source of Pichia anomaly is the Pichia anomaly that degrades the coffee rind efficiently and is re-isolated from commercial strains domesticated by the China Coffee Engineering Research Center in the fermentation of coffee rinds. Pichia anomala CERCC No. 3.
[0034] According to this method, experiment is carried out, and each parameter and ethanol yield are shown in ...
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