Bacterin for pleuropneumonia actinobacillus serotype 1 double-gene deletion mutant without resistance marker

Abstract

The invention belongs to animal bacterium gene engineering field, relates to form stem of Actinobacillus pleuropneumoniae APP-1-mut01 digene deletion mutants without fastness label, preparing vaccine and application thereof. The invention gets a stem of Actinobacillus pleuropneumoniae APP-1-mut01 (storing number is CCTCC NO; M207005). The stem absents two main toxin gene activity factors apxIC and apxIIC of Actinobacillus pleuropneumoniae APP-1-mut01, produces toxin albumen ApxIA and ApxIIA without toxin, but these two toxin albumen also have immunity originality. The invention also discloses producing vaccine of Actinobacillus pleuropneumoniae APP-1-mut01 using the digene deletion mutants stem and application thereof. The digene deletion vaccine can irritate pig generate protective immunity reaction to resist isogeny and nonhomology serum wild strain of actinobacillus pleuropneumoniae to prevent infection thereof.

Description

Technical field [0001] The invention belongs to the technical field of animal bacterial genetic engineering, and specifically relates to the construction, vaccine preparation and application of a double gene deletion mutant strain of Actinobacillus pleuropneumoniae serotype 1 without resistance markers Background technique [0002] Porcine contagious pleuropneumonia (PCP) is an infectious respiratory disease of pigs caused by Actinobacillus pleuropneumoniae (APP), which has caused serious economic losses to the world pig industry. Since the discovery of the PCP epidemic in my country, the majority of veterinarians have conducted a series of studies on its etiology, epidemiology, diagnosis and control, and achieved certain achievements. However, the incidence of this disease in my country is still increasing year by year, and the positive rate of some pig farms has reached more than 70%. It has become one of the main infectious diseases in intensive pig far...

AI Technical Summary

Problems solved by technology

For example, there is randomness in chemical or transposon-mediated mutation methods, which are only suitable for the screening of mutants with obvious phenotypic changes, and mutant strains that do not cause obvious phenotypic changes cannot be obtained by these methods; Source recombination leads to target gene mutation. The mutant strain obtained by the method finally contains resistance markers, and cannot be used as a vaccine strain for vaccine production because it does not meet the biological safety requirements.

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