47 results about "Low molecular weight protein" patented technology

Isolated soy proteins for producing high protein food bars having improved functionality are disclosed. The isolated soy proteins comprise both high molecular weight protein fractions and low molecular weight protein fractions. The isolated soy proteins produce high protein food bars having an improved texture and extended shelf-life as compared to conventional high protein food bars.

The invention belongs to the field of the petroleum industry, and particularly relates to a sustained-release long-acting nutrient for improving the valid period of indigenous microbial oil recovery. The invention adopts the technical principle that the conventional nutrient is prepared from water-soluble raw materials such as diammonium hydrogen phosphate, urea, ammonium nitrate, sodium nitrate, amino acid, molasses and the like. The raw materials are water-soluble, have different adsorption capacities in a stratum and different moving speeds, are easily drained and lost, and have short valid period. The nutrient is immobilized by adopting an immobilization method with hydrogel and the like and slowly released in the stratum; and organic phosphate, low molecular weight protein powder and the like are slowly hydrolyzed in the stratum to generate the nutrient required by indigenous bacteria. The using concentration of the sustained-release nutrient is 0.2 to 2.0 percent. The valid period of indigenous bacteria oil recovery is prolonged, so that the nutrient can enter the deep part of an oil deposit, and the efficiency of the indigenous microbial oil recovery is improved.

The invention relates to the technical field of a functional material, and discloses a preparation method of a magnetic mesoporous silica core-shell structure affinity microsphere based on metal ion modification. The method comprises the following steps of: 1) preparing magnetic particles from hexahydrate ferric trichloride; 2) preparing particle dispersion liquid by utilizing the magnetic particles; 3) adding ethanol solution containing ethyl silicate and titanium tetrabutoxide to the particle dispersion liquid, carrying out ultrasound, agitation and magnetic separation, and removing template molecules to obtain the magnetic mesoporous silica core-shell structure taking the magnetic particles as a core and the SiO2 as a shell. Polypeptide and protein with low molecular weight can be gathered and purified from a complicated biological sample by utilizing the magnetic mesoporous silica core-shell structure affinity microsphere based on the metal ion modification. The purification and separation processes of complicated low-abundance protein and low-molecular weight protein sample are greatly simplified by utilizing the magnetic property of an affinity material; and the operation time is also shortened.

A soy protein with a high molecular weight. The high molecular weight soy protein has desirable flavor and functional properties, such as high water solubility and emulsification and low sedimentation and viscosity. The method of manufacturing the protein uses soy flour and aggregates its low molecular weight proteins into high molecular weight proteins without using aqueous alcohol to modify the structure of the protein.

The invention discloses a kit for detecting low-abundance and low-molecular-weight protein spectrums. The kit comprises a kit box; a separator, a protein separation and enrichment material, a diluting buffer solution and a cleaning buffer solution are arranged in the kit body; and the protein separation and enrichment material is disposed in the separator and are porous silicon particles, the channel diameter of the material is 1-50nm, and the channel surface of the material has a chemically modified group which is an anion exchange group, a cation exchange group, a hydrophobic group or a polar group. The kit reaches an effect of one-step separation, enrichment and detection of low-molecular-weight proteins in a serum sample through preparing the porous silicon particles in the invention. The kit disclosed in he invention has the advantages of simplicity, portability, and accurate and fast detection method.

The invention discloses a preparation method of a low-abundance low-molecular-weight protein-rich material. The preparation method comprises the following steps of: fixing a P-type boron-doped silicon wafer in an electrolytic cell; adding ethanol and hydrofluoric acid as electrolyte; performing direct current electrolytic etching and demolding by taking the silicon wafer as an anode and a platinum electrode as a cathode; cleaning an etched and demolded porous silicon layer with ethanol, then ultrasonically crushing and blowing with nitrogen; performing ozone oxidation treatment on porous silicon particles; soaking the oxidized porous silicon particles in mixed solution of silylation reagent and organic solvent to react, then drying in a drying box, and soaking and cleaning the porous silicon particles, which are dried by evaporation, with ethanol; and selecting porous silicon with required aperture and performing surface modification by using amino silylation reagent, sulfydryl silylation reagent or epoxy silylation reagent. According to the preparation method, the effect of separating, enriching and detecting low-molecular-weight protein in a blood serum sample in one step is achieved by preparing the porous silicon particles.

The present disclosure relates to the use of host cell protein biomarkers to assess disulfide bond reduction in compositions comprising a protein of interest. In some embodiments, the disclosure relates to methods of predicting the occurrence of disulfide bond reduction or low molecular weight protein species in compositions comprising a protein of interest, wherein the expression or activity level of at least one host cell protein is measured and provides a benchmark value associated with the occurrence of disulfide bond reduction or low molecular weight species of said protein of interest. In some embodiments, the disclosure relates to methods of producing a protein of interest, wherein host cells capable of producing the protein of interest are cultured, the expression or activity level of at least one host cell protein is measured, and downstream isolation of the protein of interest is informed by the host cell protein measurements.

The invention discloses a PM2.5 air filter element and a preparation method thereof, and relates to the field of air purification. The PM2.5 air filter element comprises a filter element body and a PM2.5 capturing agent sprayed and coated on the filter element body, wherein the PM2.5 capturing agent uses water-soluble neutral or weak alkaline amino acid, polypeptide or low-molecular-weight protein as main raw materials; hydrophilic polymers and salt are used as the assistance; surfactants and polyamino compound are compounded for preparation. The dust can be effectively filtered; the capturing effect on organic harmful substances and sol type PM2.5 is obvious; the air purification effect is good; the cost is low; the filter element replacement or cleaning operation is simple. The PM2.5 air filter element can be widely applied to equipment such as an automobile air filter element or air purifier; the practical values are high.

[Problem ] Provided is a means for detecting and quantifying a biological substance of interest with an improved accuracy by inhibiting non-specific adsorption of fluorescent nanoparticles and thereby reducing the background noise in immunostaining with fluorescent nanoparticles. [Means for Solution] Immunostaining is carried out upon diluting fluorescent nanoparticles with a fluorescent nanoparticle diluent which contains 1 to 5% (W/W) of a protein having a molecular weight of 40,000 or higher (e,g., BSA) and 1 to 3% (W/W) of a protein having a molecular weight of less than 40,000 (eg ., casein) and, when casein is used as a low-molecular-weight protein, it is preferred that the κ-casein content in the casein is 10% (W/W) or less and the ratio of α-casein and β-casein (α-casein:β-casein) contained in the casein is 40:60 to 60:40 ( taking the total amount of α-casein and β-casein as 100).

A blood purifying apparatus having excellent purification performance (elimination of low molecular proteins, etc.) and being contaminated with little endotoxins flowing from the dialyzate side. Attention was paid to the solute permeability coefficient alpha and water permeability performance Lp of blood purifying apparatus and the relation between alpha and Lp has been examined. As a result, a blood purifying apparatus having excellent purification performance and substantially being free from invasion of endotoxins was obtained by regulating the value alpha /Lp to 6x10<-7> or above, or regulating alpha within a range of 8x10<-5> to 1.5x10<-3>, and the value alpha x Lp to 2.4x10<-2> or less. Also, a blood purifying apparatus having excellent purification performance and substantially being free from invasion of endotoxins was obtained by regulating the invasion ratio which is obtained by the polymer invasion test to 10% or less. The blood purifying apparatus has an excellent blood purifying performance expressed typically by the elimination ratio of low-molecular weight proteins and is substantially free from invasion of endotoxins from the dialyzate side, which makes it useful in, for example, treating patients with renal dysfunction.

Protein tyrosine phosphatases (PTPs) are key regulators of metabolism and insulin signaling. As a negative regulator of insulin signaling, the low molecular weight protein tyrosine phosphatase (LMPTP) is a target for insulin resistance and related conditions. Described herein are compounds capable of modulating the level of activity of LMPTP, compositions, and methods of using these compounds and compositions.

Nanoporous materials can be used to enrich samples for subsequent analysis of substances contained in the sample. The method is shown to enrich the yield of species in the low molecular weight proteome, allowing detection of small peptides in the low nanomolar range.

Protein tyrosine phosphatases (PTPs) are key regulators of metabolism and insulin signaling. As a negative regulator of insulin signaling, the low molecular weight protein tyrosine phosphatase (LMPTP) is a target for insulin resistance and related conditions. Described herein are compounds capable of modulating the level of activity of low molecular weight protein tyrosine phosphatase (LMPTP) and compositions, and methods of using these compounds and compositions.