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Nanoporous substrates for analytical methods

a technology of nanoporous substrates and analytical methods, applied in the field of nanoporous substrates for analytical methods, can solve the problems of high-abondance substances that require expensive solutions to solve common techniques, and achieve the effect of enhancing the sensitivity of analytical instruments

Inactive Publication Date: 2014-11-20
THE OHIO STATE UNIV RES FOUND +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The invention provides a method to fractionate or separate samples using a nanoporous material substrate. The substrate can retain one component of a sample while not retaining another component. This allows for the analysis of specific components of a sample without having to collect them all. Additionally, the invention provides a method for detecting markers of physiological conditions by comparing a sample with a control. The invention also includes a kit for collecting samples and a substrate for analysis, as well as an analytical system and a probe for mass spectrometry. The technical effects of the invention include improved sensitivity and accuracy in analyzing samples and the ability to detect markers of physiological conditions.

Problems solved by technology

Various techniques for analyzing substances in a clinical sample are available, but the most common techniques require expensive solutions to resolve interference from highly abundant substances in the samples, such as albumin in the case of blood serum.

Method used

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  • Nanoporous substrates for analytical methods
  • Nanoporous substrates for analytical methods
  • Nanoporous substrates for analytical methods

Examples

Experimental program
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example 1

[0074]The citations in the brackets in this example refer to the List of References at the end of Example 1.

1. Introduction

[0075]Proteomic analysis of human plasma / serum for the early detection of cancer as well as other diseases, is an increasing area of interest for many research groups [1, 2 and references therein]. Even more attention is particularly focused on the carrier protein-bound low molecular weight molecules reputed to generate and constitute the majority of the ions that comprise distinctive MS profiles used for biomarkers discovery [3-5]. The design and the development of particles engineered to mimic carrier protein (e.g. albumin) binding and perform low molecular weight proteome (LMWP) harvesting, can therefore be of high impact in biomarker discovery [6-8].

[0076]ProteinChip Array System from Ciphergen, based on SELDI-TOF mass spectrometry, can be the most extensively used platform for the discovery of “molecular signatures” [9, 10]. Among recent technologic advance...

example 2

Fractionation of Serum Components Using Nanoporous Substrates

[0120]Numerous previously uncharacterized molecules residing within a low molecular weight circulatory proteome may provide an ongoing picture of the ongoing pathophysiology of an organism. Recently, proteomic signatures comprising low molecular weight molecules have been identified using mass spectrometry combined with bioinformatics algorithms. These demonstrations have pointed to an existence of an information archive that may contain a rich source of diagnostic information. Attempts to sequence and identify the molecules that underpin the fingerprints are currently underway. A finding that many of such low molecular weight molecules may exist bound to circulating carrier proteins may afford a new opportunity for fractionation and separation techniques prior to mass spectrometry based analysis.

[0121]Nanoporous substrates represent a new approach for the facile and reproducible fractionation and selective binding of the ...

example 3

[0174]The superscript citations in this example refer to the List of References at the end of Example 3.

Summary

[0175]The low-molecular weight region of the serum / plasma proteome (LMWP) is gaining interest as a potential source of diagnostic markers for diseases1-3. Serum LMW protein profiling4 by mass spectrometry (MS) has generally relied on surface-enhanced laser desorption / ionization time-of-flight (SELDI-TOF)5, which involves MS profiling of analytes previously adsorbed on specific chip surfaces6-9. The interference of high-molecular weight, abundant proteins present in biofluids, though, may limit sensitivity and might influence reproducibility of the analysis10. Improving selectivity of MS-based profiling by using devices allowing specific entrapment of LMW polypeptides prior to MS analysis can minimise such an interference. In this report, nanoporous surfaces were used to selectively capture LMW peptides (<15,000 Da) from human plasma. Mass spectrometry (MS) analysis of harve...

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Abstract

Nanoporous materials can be used to enrich samples for subsequent analysis of substances contained in the sample. The method is shown to enrich the yield of species in the low molecular weight proteome, allowing detection of small peptides in the low nanomolar range.

Description

PRIORITY CLAIM[0001]This application is a continuation of U.S. application Ser. No. 11 / 641,970, filed Dec. 20, 2006, which claims priority to U.S. provisional patent application No. 60 / 751,924, filed Dec. 20, 2005, and U.S. provisional patent application No. 60 / 874,959, filed Dec. 15, 2006. The entire contents of each of the above-referenced applications are incorporated herein by reference in their entirety.STATEMENT FOR FEDERALLY FUNDED RESEARCH[0002]This invention was made with government support under CO012400 and CA122864 awarded by the National Institutes of Health. The government has certain rights in this invention.FIELD OF THE INVENTION[0003]The present application relates generally to analytical devices and systems and methods of making and using thereof and, more particularly, to analytical devices and systems utilizing nanoporous materials and methods of making and using thereof.BACKGROUND INFORMATION[0004]Various techniques for analyzing substances in a clinical sample ...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): G01N1/40B01L3/00
CPCB01L3/5023G01N1/405B01D15/00B01D15/08B01J20/103B01J20/2808B01J2220/54B82Y5/00C07K1/30C07K1/34G01N33/6803Y10T436/255Y10T436/2525G01N33/68B01D15/10C07K1/22B01D15/38
Inventor FERRARI, MAUROCHENG, MARK MING-CHENG
Owner THE OHIO STATE UNIV RES FOUND
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