2 results about "Disulfide bond reduction" patented technology

This invention discloses a protein composite fiber membrane material with wet adhesion and drug delivery capabilities, and its preparation method. Protein, functional active ingredients, and a volatile conformational stabilizer are mixed and treated with a disulfide reducing agent to obtain a spinning solution. Electrospinning causes the conformational stabilizer to volatilize, while the protein maintains its untransformed conformation. Subsequently, a bioadhesive polymer interlayer is applied to the fiber membrane surface, followed by secondary spinning to form a sandwich-structured composite fiber membrane material. The material obtained by this invention undergoes a controllable conformational transition in a wet environment. The β-sheets and hydrophobic residues at the interface, in conjunction with the interlayer, disrupt the hydration barrier at the interface, achieving strong adhesion through multiple mechanisms and effectively resisting interference from dynamic physiological environments. Simultaneously, the co-spinning technology enables flexible loading and controlled release of functional molecules, providing a scalable technical platform for constructing composite patches with both physical isolation and active therapeutic functions, showing promising applications in tissue repair and drug delivery.

This invention provides a cell culture additive composition for preventing antibody disulfide bond reduction and its application, relating to the biopharmaceutical field. It comprises the following raw materials: a redox regulator, a metal ion chelator, an antioxidant, an adjuvant, and a cell protectant. This invention inhibits antibody disulfide bond reduction from multiple aspects through the synergistic effect of the redox regulator, metal ion chelator, antioxidant, and buffer. Specifically, the redox regulator maintains a suitable redox potential, the metal ion chelator removes harmful metal ions, the antioxidant removes free radicals, and the buffer maintains pH stability. The components work together synergistically to enhance the effect.