25 results about "Disulfide bond reduction" patented technology

The invention discloses a protein-based adsorbent capable of efficiently adsorbing perfluorinated compounds and application of the protein-based adsorbent. The adsorbent is obtained by coating polyethyleneimine in a phase transition aggregate formed by unfolding protein through a disulfide bond reducing agent. The use method of the adsorbent comprises the step of feeding the adsorbent into a solution containing the perfluorinated compound to realize efficient adsorption of the perfluorinated compound. The adsorbent has the advantages of being low in cost, efficient, easy to operate, environmentally friendly, free of secondary pollution, good in biocompatibility and the like, efficient selective adsorption of perfluorinated compounds can be achieved through the adsorbent, the adsorbent can be repeatedly used, and industrial application and popularization are easy.

The application discloses a method for inhibiting the migration of toxic small molecules in plastics based on a protein-based phase transition composite coating, which is characterized by the following steps: inducing the rapid phase transition of proteins on the surface of plastics by a disulfide bond reducing agent to form a nanofilm, and adsorbing polysaccharides on the surface of the nanofilm to form a protein-based phase transition composite coating on the surface of the plastics, which can inhibit the migration of toxic small molecules in the plastics. The preparation method of the protein-based phase transition composite coating is simple, the cost is low, the inhibition effect on the migration of phthalate plasticizers and bisphenol A in plastics (such as PVC, PP, PE, PS and PET) is good, the adsorption of drugs on the surface of medical plastics can be prevented at the same time, the protein-based phase transition composite coating has good biocompatibility, is suitable for temperature-sensitive plastics, has universality, can be safely used on the surface of food and medical plastic products, and has a good popularization and application prospect on the surface of plastics.

The invention discloses a molecularly imprinted adsorption material taking a metal organic framework as a carrier skeleton as well as a preparation method and application of the molecularly imprinted adsorption material, and belongs to the technical field of adsorption materials. The preparation method comprises the following steps: carrying out hydrothermal reaction on transition metal salt and a phthalic acid ligand to obtain metal organic framework nanoparticles; the preparation method comprises the following steps: adsorbing flavonoid template molecules by metal organic framework nanoparticles, mixing with lysozyme and a disulfide bond reducing agent, performing phase change polymerization, and eluting to remove the flavonoid template molecules after the phase change polymerization is completed, thereby obtaining the molecularly imprinted adsorbing material with abundant imprinting sites and good stability. The adsorbent is good in flavonoid compound adsorption selectivity, high in rate, large in capacity, good in chemical stability, resistant to acid washing, capable of being recycled for multiple times and capable of being widely applied to extraction and separation of flavonoid compounds.

The invention concerns methods and means for preventing the reduction of disulfide bonds during the recombinant production of disulfide-containing polypeptides. In particular, the invention concerns the prevention of disulfide bond reduction during harvesting of disulfide-containing polypeptides, including antibodies, from recombinant host cell cultures.

The invention discloses a zirconium ion-loaded phase transition protein-based defluorination adsorbent and application thereof. The adsorbent is prepared based on zirconium ion-loaded phase transition protein membrane surface modification solid waste particles. According to the adsorbent, zirconium ions are loaded on a phase transformation protein film formed after phase transformation of protein is induced by a disulfide bond reducing agent, solid waste particles are subjected to surface modification treatment, and the solid waste particles are prepared into the efficient fluorine ion adsorbent, namely waste is treated with waste. The adsorbent has the advantages of being low in cost, high in universality, efficient, easy to operate, environmentally friendly, free of secondary pollution, good in biocompatibility and the like, efficient selective adsorption of fluorine ions can be achieved, the adsorbent can be repeatedly used, and industrial application and popularization are easy.

The present disclosure relates to the use of host cell protein biomarkers to assess disulfide bond reduction in compositions comprising a protein of interest. In some embodiments, the disclosure relates to methods of predicting the occurrence of disulfide bond reduction or low molecular weight protein species in compositions comprising a protein of interest, wherein the expression or activity level of at least one host cell protein is measured and provides a benchmark value associated with the occurrence of disulfide bond reduction or low molecular weight species of said protein of interest. In some embodiments, the disclosure relates to methods of producing a protein of interest, wherein host cells capable of producing the protein of interest are cultured, the expression or activity level of at least one host cell protein is measured, and downstream isolation of the protein of interest is informed by the host cell protein measurements.

The present invention is directed to a method of selectively reacting and removing disulfides and reducing corrosivity in petroleum matrixes with an acid wash composition.

The present disclosure provides compositions and methods for sample processing, particularly for oligonucleotide analysis e.g. analysis of formulated nucleic acid drugs. A composition for pretreating at least one target nucleic acid in a biological mixture provided herein includes a chaotropic agent selected from a substituted guanidine, a substituted amidine, a substituted quaternary amine, or a combination thereof, an optional protease, and / or an optional disulfide-reducing agent. Methods of analyzing at least one target nucleic acid in a biological mixture is also provided herein. Furthermore, the present disclosure provides methods for quantifying at least one target cationic lipid interacting with a nucleic acid.

This invention discloses a protein composite fiber membrane material with wet adhesion and drug delivery capabilities, and its preparation method. Protein, functional active ingredients, and a volatile conformational stabilizer are mixed and treated with a disulfide reducing agent to obtain a spinning solution. Electrospinning causes the conformational stabilizer to volatilize, while the protein maintains its untransformed conformation. Subsequently, a bioadhesive polymer interlayer is applied to the fiber membrane surface, followed by secondary spinning to form a sandwich-structured composite fiber membrane material. The material obtained by this invention undergoes a controllable conformational transition in a wet environment. The β-sheets and hydrophobic residues at the interface, in conjunction with the interlayer, disrupt the hydration barrier at the interface, achieving strong adhesion through multiple mechanisms and effectively resisting interference from dynamic physiological environments. Simultaneously, the co-spinning technology enables flexible loading and controlled release of functional molecules, providing a scalable technical platform for constructing composite patches with both physical isolation and active therapeutic functions, showing promising applications in tissue repair and drug delivery.

Provided are antibody-drug conjugates, methods for preparing them, and uses thereof. The antibody-drug conjugates comprise an antibody, a cytotoxic payload, and a linker of Formula I, wherein the succinimidyl group of the linker of Formula I forms a thioether bond with a thiol group obtained by reduction of the interchain disulfide chain of the antibody, and the carbonyl group in the ester group of the linker is bonded to the amino group of the payload. The antibody-drug conjugates are used in the manufacture of therapeutic agents for the diagnosis, prevention, and treatment of neoplastic diseases.

This invention provides a cell culture additive composition for preventing antibody disulfide bond reduction and its application, relating to the biopharmaceutical field. It comprises the following raw materials: a redox regulator, a metal ion chelator, an antioxidant, an adjuvant, and a cell protectant. This invention inhibits antibody disulfide bond reduction from multiple aspects through the synergistic effect of the redox regulator, metal ion chelator, antioxidant, and buffer. Specifically, the redox regulator maintains a suitable redox potential, the metal ion chelator removes harmful metal ions, the antioxidant removes free radicals, and the buffer maintains pH stability. The components work together synergistically to enhance the effect.

The present invention relates to the field of biomedical technologies, and particularly to a biomarker for diagnosing and predicting the prognosis of triple-negative breast cancer and its applications. Through bioinformatics analysis of the gene expression of triple-negative breast cancer compared with other types of breast cancer, the present invention screens out the gene FAISL that is highly expressed in triple-negative breast cancer and is closely related to the prognosis, which can be used as a new molecular biomarker for triple-negative breast tumors. Its high expression indicates a poor prognosis of the tumor. This gene can also be used as a therapeutic target for tumors. The present invention uses a disulfide bond reduction-responsive nanomaterial to encapsulate siRNA targeting the pro-cancer gene FAISL. Due to the characteristics of the nanocarrier having a long blood circulation time and strong tumor enrichment ability, the nanoparticles loaded with siRNA can significantly inhibit the expression of the pro-cancer gene, inhibit the growth and metastasis of tumor cells, and reduce the damage to normal tissue cells.

The invention discloses a sulfydryl primer synthesis method for a biological chip, and belongs to the field of biological chips, the sulfydryl primer synthesis method comprises the following steps: S1, preparing a primer containing an amino group and an acetonitrile solution; s2, preparing a sodium bicarbonate solution; s3, preparing a disulfide cross-linking agent; s4, adding an acetonitrile solution and the primer containing the amino group into a sodium bicarbonate solution to form a mixed solution; s5, adding a disulfide cross-linking agent into the mixed solution obtained in the step S4, and oscillating for condensation reaction to obtain a compound; s6, the compound obtained in the step S5 is subjected to disulfide bond reduction reaction and purification, due to the electron withdrawing effect of a benzene ring, the electrophilic activity of sulfydryl can be greatly improved, and therefore the reaction efficiency of the sulfydryl and bromine monomers on the surface of the biochip is remarkably improved, the problems that the biochip is poor in coupling stability, poor in specificity and the like can be solved, and the biochip coupling efficiency is improved. Therefore, the sensitivity and the accuracy of biochip detection are improved.

The invention concerns methods and means for preventing the reduction of disulfide bonds during the recombinant production of disulfide-containing polypeptides. In particular, the invention concerns the prevention of disulfide bond reduction during harvesting of disulfide-containing polypeptides, including antibodies, from recombinant host cell cultures.

Disclosed are methods for testing the presence and / or activity of glutathione system components and thioredoxin system components during the manufacturing process of disulfide bond-containing proteins. Also disclosed are methods for mitigating reduction of disulfide bonds during the manufacturing process, and for lowering the reduction potential of disulfide bond-containing proteins. Provided are compositions, kits, and methods for mitigating reduction and diminishing reduction potential of disulfide bond-containing proteins during protein manufacturing processes.

The invention discloses a protein-based photo-thermal super-hydrophobic coating and a preparation method and application thereof.The preparation method comprises the steps that a photo-thermal isocompound and protein are dissolved in trifluoroethanol, an obtained mixed solution and a disulfide bond reducing agent solution are mixed to form a coating on the surface of any base material, then a normal hexane solution of a hydrophobic substance is spin-coated on the surface of the coating, and the protein-based photo-thermal super-hydrophobic coating is obtained. And after the n-hexane is completely volatilized, obtaining the uniform protein-based photo-thermal super-hydrophobic coating. By means of isomerization of amyloid protein confinement photo-thermal isocompounds, the coating can achieve photo-thermal conversion under ultraviolet / visible light, on the basis, the super-hydrophobic coating is combined with solar heating, the surface temperature is increased by absorbing solar radiation, and dynamic anti-icing, static icing delay and ice adhesion strength reduction can be achieved. The coating disclosed by the invention shows excellent super-hydrophobicity and good light-induced temperature rise property, has recyclability and stability, and has a wide application prospect in all-weather anti-icing and deicing aspects in daily life.

The invention belongs to the technical field of biological medicine, and particularly relates to application of a disulfide bond reducing agent to prevention and treatment of ultraviolet-related skin injury. The ultraviolet ray is UVB, and the disulfide bond reducing agent comprises TCEP. Researches prove that TCEP can relieve the UVB injury pathological process through the disulfide bond breaking capacity and ROS removal characteristic of TCEP. Specifically, the TCEP is locally used, so that cell death caused by acute and chronic UVB exposure in human in-vitro skin tissues can be effectively relieved; tCEP treatment can effectively relieve acute and chronic UVB induced and related skin injuries in vivo; the TCEP effectively blocks keratinocyte cell death induced by UVB in vitro. The invention provides a new direction for treatment of UVB related skin injury and skin diseases.

Provided are a reagent kit and use thereof in sequencing. The reagent kit includes a first reagent and a second reagent. The first reagent is selected from a disulfide bond reducing agent. The second reagent is selected from a blocking reagent suited to blocking a disulfide bond.

The invention provides a preparation method of high-solubility rice protein, which comprises the following steps: ultrasonically dispersing rice protein powder in deionized water, and treating by a pulsed magnetic field to obtain rice protein dispersion liquid; introducing liquid nitrogen into the rice protein dispersion liquid, immediately contacting high-pressure steam with the rice protein dispersion liquid, instantaneously releasing the pressure to normal pressure, naturally cooling to room temperature, adding a disulfide bond reducing agent, and stirring at room temperature for reaction to obtain depolymerized rice protein dispersion liquid; the disulfide bond reducing agent is prepared by mixing sodium sulfite, cysteine and tea polysaccharide-selenium nanoparticles in equal mass; and adding pullulan into the depolymerized rice protein dispersion liquid, carrying out electron beam irradiation and microwave irradiation, naturally cooling to room temperature, carrying out high-pressure homogenization treatment, and carrying out spray drying.

The invention discloses a protein-based mineralization inhibitor with an anti-fouling function. The protein-based mineralization inhibitor is prepared from phosphorylated protein, a disulfide bond reducing agent and a pH regulator. According to the mineralization inhibitor, a flat protein-based coating can be obtained at an interface through methods of soaking, coating and the like; the deposition of hydroxyapatite and derivative biominerals thereof can be inhibited in a physiological environment, so that diseases such as osteoarthritis and dental calculus caused by abnormal mineralization are reduced. Besides, after a coating is formed by the mineralization inhibitor, the mineralization inhibitor has excellent anti-pollution capacity, impure protein and heteropolysaccharide in body fluid can be prevented from being adhered to the coating, and the phenomenon that the calcification inhibition capacity disappears due to pollutant adhesion is prevented. The mineralization inhibitor is non-toxic and non-irritant, and has a wide clinical application prospect.

Described herein is a process using a novel composition for strengthening hair by crosslinking the free thiols naturally present on hair or resulting from the reduction of disulfide bonds in damaged or treated hair. The process involves applying a composition containing an active agent that interacts with free thiols present in hair, forming a covalent carbon-sulphur bond, thereby enhancing the structural integrity of hair. The present invention provides a rinse off or leave-in treatment that effectively reduces breakage and restores strength to weakened or damaged hair and helps in increased longevity of reshaped hair such as straightening or curling.

The invention discloses a protein amyloid fiber with a hierarchical structure and a preparation method thereof.The preparation method comprises the steps that firstly, protein is subjected to phase transformation under induction of a disulfide bond reducing agent to form a protein nano-film, then the nano-film floats on the surface of deionized water and is compressed in the single direction to form a beta-folded and enriched fiber precursor, and the beta-folded and enriched fiber precursor is obtained; and then longitudinally stretching to induce beta-fold ordered arrangement, controlling the tensile strain to be 50%-400%, and drying while stretching to obtain the crystalline-state amyloid fiber with the hierarchical structure. According to the method, through two-step conversion of mechanical orientation, accurate regulation and control of the fiber structure and performance are achieved, and the obtained protein amyloid fiber has high orderliness and a multi-layer structure, shows excellent mechanical performance and permeability and good biological safety, has humidity responsiveness and can be used for preparing the protein amyloid fiber. The method has a wide application prospect in the fields of biomedicine and engineering.

The invention relates to a method for preventing disulfide bond reduction in protein and application thereof, and the method for preventing disulfide bond reduction in protein comprises the following steps: introducing oxygen and nitrogen into a solution containing protein, and adjusting the dissolved oxygen level in the solution to reach a preset value. The concentration difference of oxygen on a gas-liquid interface is increased through the mixed gas of oxygen and nitrogen, so that the effect of quickly reaching a set DO level under the condition that KLa cannot be increased is achieved, and two specific control models are provided, so that the method is applied to determining a ventilation strategy for large-scale production of antibodies.

The invention discloses a protein electrostatic spinning fibrous membrane dressing for promoting scar-free healing of wounds. The protein electrostatic spinning fibrous membrane dressing comprises water-soluble globular protein, a disulfide bond reducing agent and a stabilizer (trifluoroethanol, a urea water solution, trifluoroacetic acid and the like). According to the dressing, disulfide bonds of water-soluble globular protein are broken through a disulfide bond reducing agent to form an expanded protein chain, and then the expanded protein chain is stabilized through a stabilizer; electrostatic spinning is carried out by controlling the direct-current voltage between the spray head and the roller receiver rotating at a high speed, the receiving distance and the rotating speed of the roller receiver, and the protein nanofiber membrane dressing with the oriented arrangement structure is prepared. The protein nanofiber membrane dressing inhibits excessive wound contraction by reducing expression of alpha-SMA, promotes wound healing at the same time, and has a remarkable anti-scar effect. The dressing has a wide application prospect in wound treatment, especially in clinical application of pursuing scar-free healing.

The invention concerns methods for preventing the reduction of disulfide bonds during the recombinant production of disulfide-containing polypeptides. In particular, the invention concerns the prevention of disulfide bond reduction during harvesting of disulfide-containing polypeptides, including antibodies, from recombinant host cell cultures.