13 results about "Disulfide bond reduction" patented technology

The application discloses a method for inhibiting the migration of toxic small molecules in plastics based on a protein-based phase transition composite coating, which is characterized by the following steps: inducing the rapid phase transition of proteins on the surface of plastics by a disulfide bond reducing agent to form a nanofilm, and adsorbing polysaccharides on the surface of the nanofilm to form a protein-based phase transition composite coating on the surface of the plastics, which can inhibit the migration of toxic small molecules in the plastics. The preparation method of the protein-based phase transition composite coating is simple, the cost is low, the inhibition effect on the migration of phthalate plasticizers and bisphenol A in plastics (such as PVC, PP, PE, PS and PET) is good, the adsorption of drugs on the surface of medical plastics can be prevented at the same time, the protein-based phase transition composite coating has good biocompatibility, is suitable for temperature-sensitive plastics, has universality, can be safely used on the surface of food and medical plastic products, and has a good popularization and application prospect on the surface of plastics.

The invention discloses a zirconium ion-loaded phase transition protein-based defluorination adsorbent and application thereof. The adsorbent is prepared based on zirconium ion-loaded phase transition protein membrane surface modification solid waste particles. According to the adsorbent, zirconium ions are loaded on a phase transformation protein film formed after phase transformation of protein is induced by a disulfide bond reducing agent, solid waste particles are subjected to surface modification treatment, and the solid waste particles are prepared into the efficient fluorine ion adsorbent, namely waste is treated with waste. The adsorbent has the advantages of being low in cost, high in universality, efficient, easy to operate, environmentally friendly, free of secondary pollution, good in biocompatibility and the like, efficient selective adsorption of fluorine ions can be achieved, the adsorbent can be repeatedly used, and industrial application and popularization are easy.

The present invention is directed to a method of selectively reacting and removing disulfides and reducing corrosivity in petroleum matrixes with an acid wash composition.

The present disclosure provides compositions and methods for sample processing, particularly for oligonucleotide analysis e.g. analysis of formulated nucleic acid drugs. A composition for pretreating at least one target nucleic acid in a biological mixture provided herein includes a chaotropic agent selected from a substituted guanidine, a substituted amidine, a substituted quaternary amine, or a combination thereof, an optional protease, and / or an optional disulfide-reducing agent. Methods of analyzing at least one target nucleic acid in a biological mixture is also provided herein. Furthermore, the present disclosure provides methods for quantifying at least one target cationic lipid interacting with a nucleic acid.

This invention discloses a protein composite fiber membrane material with wet adhesion and drug delivery capabilities, and its preparation method. Protein, functional active ingredients, and a volatile conformational stabilizer are mixed and treated with a disulfide reducing agent to obtain a spinning solution. Electrospinning causes the conformational stabilizer to volatilize, while the protein maintains its untransformed conformation. Subsequently, a bioadhesive polymer interlayer is applied to the fiber membrane surface, followed by secondary spinning to form a sandwich-structured composite fiber membrane material. The material obtained by this invention undergoes a controllable conformational transition in a wet environment. The β-sheets and hydrophobic residues at the interface, in conjunction with the interlayer, disrupt the hydration barrier at the interface, achieving strong adhesion through multiple mechanisms and effectively resisting interference from dynamic physiological environments. Simultaneously, the co-spinning technology enables flexible loading and controlled release of functional molecules, providing a scalable technical platform for constructing composite patches with both physical isolation and active therapeutic functions, showing promising applications in tissue repair and drug delivery.

Provided are antibody-drug conjugates, methods for preparing them, and uses thereof. The antibody-drug conjugates comprise an antibody, a cytotoxic payload, and a linker of Formula I, wherein the succinimidyl group of the linker of Formula I forms a thioether bond with a thiol group obtained by reduction of the interchain disulfide chain of the antibody, and the carbonyl group in the ester group of the linker is bonded to the amino group of the payload. The antibody-drug conjugates are used in the manufacture of therapeutic agents for the diagnosis, prevention, and treatment of neoplastic diseases.

This invention provides a cell culture additive composition for preventing antibody disulfide bond reduction and its application, relating to the biopharmaceutical field. It comprises the following raw materials: a redox regulator, a metal ion chelator, an antioxidant, an adjuvant, and a cell protectant. This invention inhibits antibody disulfide bond reduction from multiple aspects through the synergistic effect of the redox regulator, metal ion chelator, antioxidant, and buffer. Specifically, the redox regulator maintains a suitable redox potential, the metal ion chelator removes harmful metal ions, the antioxidant removes free radicals, and the buffer maintains pH stability. The components work together synergistically to enhance the effect.

The invention concerns methods and means for preventing the reduction of disulfide bonds during the recombinant production of disulfide-containing polypeptides. In particular, the invention concerns the prevention of disulfide bond reduction during harvesting of disulfide-containing polypeptides, including antibodies, from recombinant host cell cultures.

Disclosed are methods for testing the presence and / or activity of glutathione system components and thioredoxin system components during the manufacturing process of disulfide bond-containing proteins. Also disclosed are methods for mitigating reduction of disulfide bonds during the manufacturing process, and for lowering the reduction potential of disulfide bond-containing proteins. Provided are compositions, kits, and methods for mitigating reduction and diminishing reduction potential of disulfide bond-containing proteins during protein manufacturing processes.

The invention belongs to the technical field of biological medicine, and particularly relates to application of a disulfide bond reducing agent to prevention and treatment of ultraviolet-related skin injury. The ultraviolet ray is UVB, and the disulfide bond reducing agent comprises TCEP. Researches prove that TCEP can relieve the UVB injury pathological process through the disulfide bond breaking capacity and ROS removal characteristic of TCEP. Specifically, the TCEP is locally used, so that cell death caused by acute and chronic UVB exposure in human in-vitro skin tissues can be effectively relieved; tCEP treatment can effectively relieve acute and chronic UVB induced and related skin injuries in vivo; the TCEP effectively blocks keratinocyte cell death induced by UVB in vitro. The invention provides a new direction for treatment of UVB related skin injury and skin diseases.

Described herein is a process using a novel composition for strengthening hair by crosslinking the free thiols naturally present on hair or resulting from the reduction of disulfide bonds in damaged or treated hair. The process involves applying a composition containing an active agent that interacts with free thiols present in hair, forming a covalent carbon-sulphur bond, thereby enhancing the structural integrity of hair. The present invention provides a rinse off or leave-in treatment that effectively reduces breakage and restores strength to weakened or damaged hair and helps in increased longevity of reshaped hair such as straightening or curling.

The invention discloses a protein electrostatic spinning fibrous membrane dressing for promoting scar-free healing of wounds. The protein electrostatic spinning fibrous membrane dressing comprises water-soluble globular protein, a disulfide bond reducing agent and a stabilizer (trifluoroethanol, a urea water solution, trifluoroacetic acid and the like). According to the dressing, disulfide bonds of water-soluble globular protein are broken through a disulfide bond reducing agent to form an expanded protein chain, and then the expanded protein chain is stabilized through a stabilizer; electrostatic spinning is carried out by controlling the direct-current voltage between the spray head and the roller receiver rotating at a high speed, the receiving distance and the rotating speed of the roller receiver, and the protein nanofiber membrane dressing with the oriented arrangement structure is prepared. The protein nanofiber membrane dressing inhibits excessive wound contraction by reducing expression of alpha-SMA, promotes wound healing at the same time, and has a remarkable anti-scar effect. The dressing has a wide application prospect in wound treatment, especially in clinical application of pursuing scar-free healing.

The invention concerns methods for preventing the reduction of disulfide bonds during the recombinant production of disulfide-containing polypeptides. In particular, the invention concerns the prevention of disulfide bond reduction during harvesting of disulfide-containing polypeptides, including antibodies, from recombinant host cell cultures.