Prevention of disulfide bond reduction during recombinant production of polypeptides

a technology of disulfide bond and recombinant protein, which is applied in the direction of peptides, drug compositions, immunological disorders, etc., can solve the problems that the production of recombinant proteins is still not without difficulties, and achieve the effect of preventing the reduction of a disulfide bond
US20090053786A1Inactive Publication Date: 2009-02-26GENENTECH INC
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Patent Information

Authority / Receiving Office
US · United States
Patent Type
Applications(United States)
Current Assignee / Owner
GENENTECH INC
Publication Date
2009-02-26
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention concerns methods and means for preventing the reduction of disulfide bonds during the recombinant production of disulfide-containing polypeptides. In particular, the invention concerns the prevention of disulfide bond reduction during harvesting of disulfide-containing polypeptides, including antibodies, from recombinant host cell cultures.
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Description

CROSS REFERENCE TO RELATED APPLICATIONS

[0001] This application is a non-provisional application filed under 37 CFR 1.53(b)(1), claiming priority under 35 USC 119(e) to provisional application No. 60 / 948,677 filed Jul. 9, 2007, the contents of which are incorporated herein by reference.FIELD OF THE INVENTION

[0002] The invention concerns methods and means for preventing the reduction of disulfide bonds during the recombinant production of disulfide-containing polypeptides. In particular, the invention concerns the prevention of disulfide bond reduction during harvesting of disulfide-containing polypeptides, including antibodies, from recombinant host cell cultures.BACKGROUND OF THE INVENTION

[0003] In the biotechnology industry, pharmaceutical applications require a variety of proteins produced using recombinant DNA techniques. Generally, recombinant proteins are produced by cell culture, using either eukaryotic cells, such as mammalian cells, or prokaryotic cells, such as bacterial cells...

Claims

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