The invention discloses a biological chip for rapidly detecting pre-pregnant intrauterine toxoplasma gondii and cytomegalovirus infection. Compared with an antibody in traditional detection technique, an oligonucleotides adapter has the advantages that the binding force between the oligonucleotides adapter and target molecule is higher, stability is good, accuracy is high, repeatability is good, screening period is shorter, can be repeatedly used, can be stored for long time, can be precisely modified at sites, and the like; and by placing the obtained biological chip in a SPR (surface plasmon resonance) biosensor for detection, (1) the real-time detection is realized, (2) the sample does not need to be labeled, (3) the consumption of the sample is extremely low, (4) the detection processis convenient and efficient, and has high sensitivity, (5) the application range is very broad, (6) the flux is high, (7) in most cases, the sample does not need to be pretreated, and (8) the turbid or even nontransparent samples can be detected.