Dual-channel optical fiber SPR biosensor

A biosensor, dual-channel technology, applied in the field of biosensors, can solve the problems of low sensitivity and accuracy of optical fiber SPR biosensors, and achieve the effects of being beneficial to biosensing, high sensitivity, and eliminating measurement errors

Pending Publication Date: 2018-12-21
NORTHEASTERN UNIV
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Problems solved by technology

[0004] In order to solve the problem of low sensitivity and accuracy of existing optical f

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  • Dual-channel optical fiber SPR biosensor
  • Dual-channel optical fiber SPR biosensor
  • Dual-channel optical fiber SPR biosensor

Examples

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Embodiment 1

[0056] In this example, a dual-channel optical fiber SPR biosensor for detecting human immunoglobulin was prepared, that is, the antigen to be detected was human immunoglobulin, and the detected antibody was goat anti-human immunoglobulin.

[0057] Such as figure 1 As shown, a dual-channel optical fiber SPR biosensor includes a reference channel 1' and a sensing channel 2', and the reference channel 1' and the detection channel 2' are connected by a multimode optical fiber, wherein:

[0058] The reference channel 1' includes a first photonic crystal fiber 1, the length of the first photonic crystal fiber 1 is 0.5-2 cm, and the two ends of the first photonic crystal fiber 1 are respectively welded with a multimode fiber 2; the surface of the first photonic crystal fiber 1 is coated with silver film 4. The thickness of the silver film 4 is 40-60nm; the reference channel 1' is used as a blank control to detect the wavelength shift caused by non-specific adsorption;

[0059] The ...

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Abstract

The invention relates to a dual-channel optical fiber SPR sensor. The dual-channel SPR sensor comprises a reference channel and a sensing channel; the reference channel serves as a blank control reference channel and detects a wavelength shift quantity caused by non-specific adsorption; and the sensing channel improves the detection sensitivity of the sensor by enhancing the intensity of a local electric field through utilizing a coupling effect between a gold film and gold nanoparticles, and improves the immobilization efficiency of an antibody by utilizing a graphene oxide thin film. The twochannels in the dual-channel optical fiber SPR sensor use different metal films respectively, so that two resonance valleys are generated, double-channel sensing is realized, and the unique advantages of eliminating a measuring error caused by the non-specific adsorption and being insensitive to the temperature are achieved; and the two SPR resonance valleys of the two channels are separated fromeach other, so that the SPR resonance valleys are prevented from generating the adverse effects mutually in the detection process, and biological sensing is better facilitated. According to the sensor, the problems that the sensor is low in sensitivity and easy to influence by liquid refractive index change and temperature fluctuation in the prior art are effectively solved.

Description

technical field [0001] The invention relates to the technical field of biosensors, in particular to an SPR biosensor, in particular to a dual-channel optical fiber SPR biosensor. Background technique [0002] Surface Plasmon Resonance (SPR), the Chinese name is Surface Plasmon Resonance, it is a common optical phenomenon, it refers to when the incident light of a certain wavelength in the optical waveguide irradiates the metal (such as gold or silver) film, the light wave It will resonate with the plasma wave generated on the metal surface, which is called the surface plasmon resonance (SPR) effect. The SPR effect will sharply reduce the energy of the reflected light, thereby forming a resonance trough. At the same time, SPR is very sensitive to the external refractive index. When the concentration of the measured solution changes, that is, the external solution refractive index changes, the SPR resonance trough will follow. Movement, therefore, the present invention enable...

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Application Information

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IPC IPC(8): G01N21/552G01N33/68G01N33/543
CPCG01N21/554G01N33/54346G01N33/6854
Inventor 王琦王雪州王波涛赵万明井建迎汪颖
Owner NORTHEASTERN UNIV
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